Tuning the properties of a novel short cell-penetrating peptide by intramolecular cyclization with a triazole bridge

2016 ◽  
Vol 52 (11) ◽  
pp. 2261-2264 ◽  
Author(s):  
M. Horn ◽  
F. Reichart ◽  
S. Natividad-Tietz ◽  
D. Diaz ◽  
I. Neundorf
Keyword(s):  
Intramolecular Cyclization ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
Membrane Insertion ◽  
Short Cell ◽  
Cell Penetrating

Cyclic versus linear: cyclic triazole-bridged cell-penetrating peptides are optimally arranged within the membrane, thus at the same time inducing suitable DNA complexation and successful peptide membrane insertion.

Enhanced oral absorption of insulin using colon-specific nanoparticles co-modified with amphiphilic chitosan derivatives and cell-penetrating peptides

2019 ◽  
Vol 7 (4) ◽  
pp. 1493-1506 ◽  
Author(s):  
Feng Guo ◽  
Ting Ouyang ◽  
Taoxing Peng ◽  
Xiuying Zhang ◽  
Baogang Xie ◽  
...  
Keyword(s):  
Oral Absorption ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
Chitosan Derivative ◽  
Chitosan Derivatives ◽  
Cell Penetrating

In this study, amphipathic chitosan derivative (ACS) and cell-penetrating peptide (CPP) co-modified colon-specific nanoparticles (CS-CPP NPs) were prepared and evaluated.

scholarly journals siRNA suppression of hTERT using activatable cell-penetrating peptides in hepatoma cells

2015 ◽  
Vol 35 (2) ◽  
Author(s):  
Hua Li ◽  
Jiwen He ◽  
Huimin Yi ◽  
Guoan Xiang ◽  
Kaiyun Chen ◽  
...  
Keyword(s):  
Hepatoma Cells ◽  
Cell Penetrating Peptides ◽  
Matrix Metalloproteinase 2 ◽  
Cell Penetrating Peptide ◽  
G1 Arrest ◽  
Telomerase Reverse Transcriptase ◽  
Human Telomerase Reverse Transcriptase ◽  
Htert Gene ◽  
Cell Penetrating ◽  
Human Telomerase

In the present study, we delivered human telomerase reverse transcriptase (hTERT) siRNA into SMMC-7721 hepatoma cells using a matrix metalloproteinase-2 (MMP2)-activatable cell-penetrating peptide (aCPP). The siRNA subsequently induced down-regulation of the hTERT gene and G1-arrest, implicating the utility of this delivery system in cancer therapy.

scholarly journals A comparison of acyl-moieties for noncovalent functionalization of PLGA and PEG-PLGA nanoparticles with a cell-penetrating peptide

RSC Advances ◽  
2021 ◽  
Vol 11 (57) ◽  
pp. 36116-36124
Author(s):  
Omar Paulino da Silva Filho ◽  
Muhanad Ali ◽  
Rike Nabbefeld ◽  
Daniel Primavessy ◽  
Petra H. Bovee-Geurts ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Plga Nanoparticles ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
Noncovalent Functionalization ◽  
Cell Penetrating ◽  
A Cell

Noncovalent functionalization with acylated cell-penetrating peptides achieves an efficient cellular uptake of PLGA and PEG-PLGA nanoparticles.

scholarly journals Stimuli-Sensitive Cell Penetrating Peptide-Modified Nanocarriers

Processes ◽  
2019 ◽  
Vol 7 (10) ◽  
pp. 727 ◽  
Author(s):  
Perche
Keyword(s):  
Therapeutic Index ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
Cellular Internalization ◽  
Normal Tissues ◽  
Formidable Challenge ◽  
Cell Penetrating ◽  
Stimuli Sensitive ◽  
Responsive Cell

The integration of drugs into nanocarriers favorably altered their pharmacodynamics and pharmacokinetics compared to free drugs, and increased their therapeutic index. However, selective cellular internalization in diseased tissues rather than normal tissues still presents a formidable challenge. In this chapter I will cover solutions involving environment-responsive cell-penetrating peptides (CPPs). I will discuss properties of CPPs as universal cellular uptake enhancers, and the modifications imparted to CPP-modified nanocarriers to confine CPP activation to diseased tissues.

Cell-penetrating-peptide-based delivery of oligonucleotides: an overview

2007 ◽  
Vol 35 (4) ◽  
pp. 775-779 ◽  
Author(s):  
R. Abes ◽  
A.A. Arzumanov ◽  
H.M. Moulton ◽  
S. Abes ◽  
G.D. Ivanova ◽  
...  
Keyword(s):  
Cell Penetrating Peptides ◽  
Peptide Delivery ◽  
Cell Penetrating Peptide ◽  
Cellular Internalization ◽  
Structure Activity ◽  
Cell Penetrating ◽  
Transactivator Of Transcription ◽  
Endocytic Vesicles ◽  
Oligonucleotide Analogues ◽  
Phosphorodiamidate Morpholino Oligomers

Cationic CPPs (cell-penetrating peptides) have been used largely for intracellular delivery of low-molecular-mass drugs, biomolecules and particles. Most cationic CPPs bind to cell-associated glycosaminoglycans and are internalized by endocytosis, although the detailed mechanisms involved remain controversial. Sequestration and degradation in endocytic vesicles severely limits the efficiency of cytoplasmic and/or nuclear delivery of CPP-conjugated material. Re-routing the splicing machinery by using steric-block ON (oligonucleotide) analogues, such as PNAs (peptide nucleic acids) or PMOs (phosphorodiamidate morpholino oligomers), has consequently been inefficient when ONs are conjugated with standard CPPs such as Tat (transactivator of transcription), R9 (nona-arginine), K8 (octalysine) or penetratin in the absence of endosomolytic agents. New arginine-rich CPPs such as (R-Ahx-R)4 (6-aminohexanoic acid-spaced oligo-arginine) or R6 (hexa-arginine)–penetratin conjugated to PMO or PNA resulted in efficient splicing correction at non-cytotoxic doses in the absence of chloroquine. SAR (structure–activity relationship) analyses are underway to optimize these peptide delivery vectors and to understand their mechanisms of cellular internalization.

scholarly journals The anticancer efficacy of paclitaxel liposomes modified with low-toxicity hydrophobic cell-penetrating peptides in breast cancer: an in vitro and in vivo evaluation

RSC Advances ◽  
2018 ◽  
Vol 8 (43) ◽  
pp. 24084-24093 ◽  
Author(s):  
Qi Zhang ◽  
Jing Wang ◽  
Hao Zhang ◽  
Dan Liu ◽  
Linlin Ming ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Targeted Delivery ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
In Vivo Evaluation ◽  
Anticancer Efficacy ◽  
Cell Penetrating ◽  
Low Toxicity

Hydrophobic cell penetrating peptide PFVYLI-modified liposomes have been developed for the targeted delivery of PTX into tumors.

scholarly journals A simple and efficient transfection protocol for Cryptosporidium parvum using Polyethylenimine (PEI) and Octaarginine

Parasitology ◽  
2020 ◽  
Vol 147 (10) ◽  
pp. 1065-1070
Author(s):  
Tran Nguyen-Ho-Bao ◽  
Maxi Berberich ◽  
Wanpeng Zheng ◽  
Dieter Seebach ◽  
Arwid Daugschies ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cryptosporidium Parvum ◽  
Plasmid Dna ◽  
Cationic Polymer ◽  
Cell Penetrating Peptide ◽  
Laboratory Equipment ◽  
Short Cell ◽  
Transfection Protocol ◽  
Simple Protocol ◽  
Cell Penetrating

AbstractThe transfection of Cryptosporidium represents a major challenge, and current protocols are based on electroporation of freshly excysted sporozoites using a rather large amount of plasmid DNA which typically has a very poor yield. In this study, we report a fast and simple protocol for transfection of Cryptosporidium parvum that takes advantage of the DNA condensing power of the poly cationic polymer polyethylenimine (PEI) and the gene delivery property of the short cell-penetrating peptide octaarginine. Our novel protocol requires a very low amount of plasmid DNA and does not necessitate special laboratory equipment to be performed. Transfection appears to be more efficient in oocysts just triggered for excystation than the excysted sporozoites. Altogether, the application of octaarginine with PEI allows efficient transfection. To the best of our knowledge, this is the first report on an electroporation-free protocol for transfection of sporozoites of a Cryptosporidium species.

scholarly journals Designed cell penetrating peptide dendrimers efficiently internalize cargo into cells

2014 ◽  
Vol 50 (55) ◽  
pp. 7254-7257 ◽  
Author(s):  
Gabriela A. Eggimann ◽  
Emilyne Blattes ◽  
Stefanie Buschor ◽  
Rasomoy Biswas ◽  
Stephan M. Kammer ◽  
...  
Keyword(s):  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
Cell Penetration ◽  
Lower Toxicity ◽  
Serum Stability ◽  
Peptide Dendrimers ◽  
Cell Penetrating ◽  
Linear Cell

Redesigning linear cell penetrating peptides (CPPs) into a multi-branched topology with short dipeptide branches gave cell penetrating peptide dendrimers (CPPDs) with higher cell penetration, lower toxicity and hemolysis and higher serum stability than linear CPPs.

scholarly journals Membrane Insertion Potential of Synthetic Cell Penetrating Peptides

2014 ◽  
Vol 106 (2) ◽  
pp. 97a
Author(s):  
Nabil A. Alhakamy ◽  
Anubhav Kaviratna ◽  
Cory Berkland ◽  
Prajnaparamita Dhar
Keyword(s):  
Cell Penetrating Peptides ◽  
Membrane Insertion ◽  
Synthetic Cell ◽  
Cell Penetrating
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