Enhanced oral absorption of insulin using colon-specific nanoparticles co-modified with amphiphilic chitosan derivatives and cell-penetrating peptides

2019 ◽  
Vol 7 (4) ◽  
pp. 1493-1506 ◽  
Author(s):  
Feng Guo ◽  
Ting Ouyang ◽  
Taoxing Peng ◽  
Xiuying Zhang ◽  
Baogang Xie ◽  
...  
Keyword(s):  
Oral Absorption ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
Chitosan Derivative ◽  
Chitosan Derivatives ◽  
Cell Penetrating

In this study, amphipathic chitosan derivative (ACS) and cell-penetrating peptide (CPP) co-modified colon-specific nanoparticles (CS-CPP NPs) were prepared and evaluated.

scholarly journals siRNA suppression of hTERT using activatable cell-penetrating peptides in hepatoma cells

2015 ◽  
Vol 35 (2) ◽  
Author(s):  
Hua Li ◽  
Jiwen He ◽  
Huimin Yi ◽  
Guoan Xiang ◽  
Kaiyun Chen ◽  
...  
Keyword(s):  
Hepatoma Cells ◽  
Cell Penetrating Peptides ◽  
Matrix Metalloproteinase 2 ◽  
Cell Penetrating Peptide ◽  
G1 Arrest ◽  
Telomerase Reverse Transcriptase ◽  
Human Telomerase Reverse Transcriptase ◽  
Htert Gene ◽  
Cell Penetrating ◽  
Human Telomerase

In the present study, we delivered human telomerase reverse transcriptase (hTERT) siRNA into SMMC-7721 hepatoma cells using a matrix metalloproteinase-2 (MMP2)-activatable cell-penetrating peptide (aCPP). The siRNA subsequently induced down-regulation of the hTERT gene and G1-arrest, implicating the utility of this delivery system in cancer therapy.

scholarly journals A comparison of acyl-moieties for noncovalent functionalization of PLGA and PEG-PLGA nanoparticles with a cell-penetrating peptide

RSC Advances ◽  
2021 ◽  
Vol 11 (57) ◽  
pp. 36116-36124
Author(s):  
Omar Paulino da Silva Filho ◽  
Muhanad Ali ◽  
Rike Nabbefeld ◽  
Daniel Primavessy ◽  
Petra H. Bovee-Geurts ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Plga Nanoparticles ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
Noncovalent Functionalization ◽  
Cell Penetrating ◽  
A Cell

Noncovalent functionalization with acylated cell-penetrating peptides achieves an efficient cellular uptake of PLGA and PEG-PLGA nanoparticles.

scholarly journals Stimuli-Sensitive Cell Penetrating Peptide-Modified Nanocarriers

Processes ◽  
2019 ◽  
Vol 7 (10) ◽  
pp. 727 ◽  
Author(s):  
Perche
Keyword(s):  
Therapeutic Index ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
Cellular Internalization ◽  
Normal Tissues ◽  
Formidable Challenge ◽  
Cell Penetrating ◽  
Stimuli Sensitive ◽  
Responsive Cell

The integration of drugs into nanocarriers favorably altered their pharmacodynamics and pharmacokinetics compared to free drugs, and increased their therapeutic index. However, selective cellular internalization in diseased tissues rather than normal tissues still presents a formidable challenge. In this chapter I will cover solutions involving environment-responsive cell-penetrating peptides (CPPs). I will discuss properties of CPPs as universal cellular uptake enhancers, and the modifications imparted to CPP-modified nanocarriers to confine CPP activation to diseased tissues.

Cell-penetrating-peptide-based delivery of oligonucleotides: an overview

2007 ◽  
Vol 35 (4) ◽  
pp. 775-779 ◽  
Author(s):  
R. Abes ◽  
A.A. Arzumanov ◽  
H.M. Moulton ◽  
S. Abes ◽  
G.D. Ivanova ◽  
...  
Keyword(s):  
Cell Penetrating Peptides ◽  
Peptide Delivery ◽  
Cell Penetrating Peptide ◽  
Cellular Internalization ◽  
Structure Activity ◽  
Cell Penetrating ◽  
Transactivator Of Transcription ◽  
Endocytic Vesicles ◽  
Oligonucleotide Analogues ◽  
Phosphorodiamidate Morpholino Oligomers

Cationic CPPs (cell-penetrating peptides) have been used largely for intracellular delivery of low-molecular-mass drugs, biomolecules and particles. Most cationic CPPs bind to cell-associated glycosaminoglycans and are internalized by endocytosis, although the detailed mechanisms involved remain controversial. Sequestration and degradation in endocytic vesicles severely limits the efficiency of cytoplasmic and/or nuclear delivery of CPP-conjugated material. Re-routing the splicing machinery by using steric-block ON (oligonucleotide) analogues, such as PNAs (peptide nucleic acids) or PMOs (phosphorodiamidate morpholino oligomers), has consequently been inefficient when ONs are conjugated with standard CPPs such as Tat (transactivator of transcription), R9 (nona-arginine), K8 (octalysine) or penetratin in the absence of endosomolytic agents. New arginine-rich CPPs such as (R-Ahx-R)4 (6-aminohexanoic acid-spaced oligo-arginine) or R6 (hexa-arginine)–penetratin conjugated to PMO or PNA resulted in efficient splicing correction at non-cytotoxic doses in the absence of chloroquine. SAR (structure–activity relationship) analyses are underway to optimize these peptide delivery vectors and to understand their mechanisms of cellular internalization.

scholarly journals The anticancer efficacy of paclitaxel liposomes modified with low-toxicity hydrophobic cell-penetrating peptides in breast cancer: an in vitro and in vivo evaluation

RSC Advances ◽  
2018 ◽  
Vol 8 (43) ◽  
pp. 24084-24093 ◽  
Author(s):  
Qi Zhang ◽  
Jing Wang ◽  
Hao Zhang ◽  
Dan Liu ◽  
Linlin Ming ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Targeted Delivery ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
In Vivo Evaluation ◽  
Anticancer Efficacy ◽  
Cell Penetrating ◽  
Low Toxicity

Hydrophobic cell penetrating peptide PFVYLI-modified liposomes have been developed for the targeted delivery of PTX into tumors.

scholarly journals Designed cell penetrating peptide dendrimers efficiently internalize cargo into cells

2014 ◽  
Vol 50 (55) ◽  
pp. 7254-7257 ◽  
Author(s):  
Gabriela A. Eggimann ◽  
Emilyne Blattes ◽  
Stefanie Buschor ◽  
Rasomoy Biswas ◽  
Stephan M. Kammer ◽  
...  
Keyword(s):  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
Cell Penetration ◽  
Lower Toxicity ◽  
Serum Stability ◽  
Peptide Dendrimers ◽  
Cell Penetrating ◽  
Linear Cell

Redesigning linear cell penetrating peptides (CPPs) into a multi-branched topology with short dipeptide branches gave cell penetrating peptide dendrimers (CPPDs) with higher cell penetration, lower toxicity and hemolysis and higher serum stability than linear CPPs.

Intracellular target delivery of cell-penetrating peptide-conjugated dodecaborate for boron neutron capture therapy (BNCT)

2019 ◽  
Vol 55 (93) ◽  
pp. 13955-13958 ◽  
Author(s):  
Ikuhiko Nakase ◽  
Miku Katayama ◽  
Yoshihide Hattori ◽  
Miki Ishimura ◽  
Shunsuke Inaura ◽  
...  
Keyword(s):  
Boron Neutron Capture Therapy ◽  
Neutron Capture ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
Neutron Capture Therapy ◽  
Boron Compounds ◽  
Target Delivery ◽  
Boron Neutron Capture ◽  
Cell Penetrating ◽  
Intracellular Target

Boron neutron capture therapy (BNCT) technology using cell-penetrating peptides (CPPs) for enhanced cellular uptake of boron compounds and their controlled localization inside cells.

scholarly journals Safety of the Cell-Penetrating Peptide Penetratin as an Oral Absorption Enhancer

2015 ◽  
Vol 38 (1) ◽  
pp. 144-146 ◽  
Author(s):  
Ebbe Juel Bech Nielsen ◽  
Noriyasu Kamei ◽  
Mariko Takeda-Morishita
Keyword(s):  
Oral Absorption ◽  
Absorption Enhancer ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating

scholarly journals On the mechanisms of the internalization of S413-PV cell-penetrating peptide

2005 ◽  
Vol 390 (2) ◽  
pp. 603-612 ◽  
Author(s):  
Miguel Mano ◽  
Cristina Teodósio ◽  
Artur Paiva ◽  
Sérgio Simões ◽  
Maria C. Pedroso de Lima
Keyword(s):  
Cellular Uptake ◽  
Cell Membranes ◽  
Heparan Sulphate ◽  
Cell Penetrating Peptides ◽  
Membrane Receptors ◽  
Live Cells ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Pv Cell

Cell-penetrating peptides have been shown to translocate across eukaryotic cell membranes through a temperature-insensitive and energy-independent mechanism that does not involve membrane receptors or transporters. Although cell-penetrating peptides have been successfully used to mediate the intracellular delivery of a wide variety of molecules of pharmacological interest both in vitro and in vivo, the mechanisms by which cellular uptake occurs remain unclear. In the face of recent reports demonstrating that uptake of cell-penetrating peptides occurs through previously described endocytic pathways, or is a consequence of fixation artifacts, we conducted a critical re-evaluation of the mechanism responsible for the cellular uptake of the S413-PV karyophilic cell-penetrating peptide. We report that the S413-PV peptide is able to accumulate inside live cells very efficiently through a rapid, dose-dependent and non-toxic process, providing clear evidence that the cellular uptake of this peptide cannot be attributed to fixation artifacts. Comparative analysis of peptide uptake into mutant cells lacking heparan sulphate proteoglycans demonstrates that their presence at the cell surface facilitates the cellular uptake of the S413-PV peptide, particularly at low peptide concentrations. Most importantly, our results clearly demonstrate that, in addition to endocytosis, which is only evident at low peptide concentrations, the efficient cellular uptake of the S413-PV cell-penetrating peptide occurs mainly through an alternative, non-endocytic mechanism, most likely involving direct penetration across cell membranes.

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