Raman/fluorescence dual-sensing and imaging of intracellular pH distribution

2015 ◽  
Vol 51 (99) ◽  
pp. 17584-17587 ◽  
Author(s):  
Yue Cao ◽  
Ruo-Can Qian ◽  
Da-Wei Li ◽  
Yi-Tao Long
Keyword(s):  
Gold Nanoparticles ◽  
Intracellular Ph ◽  
Ph Sensitive ◽  
Ph Distribution ◽  
Dual Imaging ◽  
Dual Sensing

A pH-sensitive probe has been designed based on DNA modified gold nanoparticles (AuNPs) for Raman/fluorescence dual-imaging of the intracellular pH distribution.

LONG TERM EFFECTS OF THYROID STIMULATING HORMONE AND INSULIN ON INTRACELLULAR pH IN FRTL-5 CELLS

1992 ◽  
Vol 133 (2) ◽  
pp. R9-R11
Author(s):  
A.M. Wood ◽  
S.P. Bidey ◽  
J. Soden ◽  
W.R. Robertson
Keyword(s):  
Intracellular Ph ◽  
Small Groups ◽  
Thyroid Stimulating Hormone ◽  
Ph Sensitive ◽  
Long Term Effects ◽  
Bicarbonate Ions ◽  
Chronic Effects ◽  
Petri Dishes ◽  
Presence And Absence

ABSTRACT We have studied the chronic effects of TSH (100μU/ml) and insulin (10μg/ml) on intracellular pH (pHi) in FRTL-5 cells using the pH sensitive probe 2′7-bis (2-carboxyethyl-5′-6′) carboxyfluorescein. FRTL-5 cells were cultured on Petri dishes either in the presence of 4H, ie. Coons F-12 containing cortisol (10nM), transferrin (0.5μg/ml), glycyl-histidyl lysine acetate (10ng/ml) and somatostatin (10μg/ml), or with 4H+insulin (5H), 4H+TSH, or 4H+TSH+insulin (6H). pHi was measured in small groups of cells by microspectrofluorimetry both in the presence and absence of bicarbonate ions after cells had been deprived of serum for at least a day. In

Fabrication and dual imaging properties of quantum dot/silica core-shell particles immobilized with gold nanoparticles

2018 ◽  
Vol 33 (11) ◽  
pp. 737-747
Author(s):  
Ting-ting Li ◽  
Tomoya Inose ◽  
Takahiro Oikawa ◽  
Masayuki Tokunaga ◽  
Keiichiro Hatoyama ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Quantum Dot ◽  
Core Shell ◽  
Silica Core ◽  
Dual Imaging ◽  
Shell Particles ◽  
Imaging Properties

Intracellular pH-Sensitive PEG-block-Acetalated-Dextrans as Efficient Drug Delivery Platforms

2013 ◽  
Vol 5 (21) ◽  
pp. 10760-10766 ◽  
Author(s):  
Zhe Zhang ◽  
Xiaofei Chen ◽  
Li Chen ◽  
Shuangjiang Yu ◽  
Yue Cao ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Intracellular Ph ◽  
Ph Sensitive

Design and validation of a new ratiometric intracellular pH imaging probe using lanthanide-doped upconverting nanoparticles

2017 ◽  
Vol 46 (40) ◽  
pp. 13957-13965 ◽  
Author(s):  
Shuoren Du ◽  
Javier Hernández-Gil ◽  
Hao Dong ◽  
Xiaoyu Zheng ◽  
Guangming Lyu ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Quantitative Imaging ◽  
Living Cells ◽  
Ph Sensitive ◽  
Upconversion Nanoparticles ◽  
Imaging Probe ◽  
Ph Imaging ◽  
Ratiometric Probe ◽  
Subcellular Level

A ratiometric probe based on upconversion nanoparticles modified with a pH sensitive moiety for the quantitative imaging of pH at the subcellular level in living cells.

Regulation of intracellular pH in proximal tubules of avian long-looped mammalian-type nephrons

1998 ◽  
Vol 274 (6) ◽  
pp. R1526-R1535 ◽  
Author(s):  
Olga H. Brokl ◽  
Christina L. Martinez ◽  
Apichai Shuprisha ◽  
Diane E. Abbott ◽  
William H. Dantzler
Keyword(s):  
Intracellular Ph ◽  
Ph Sensitive ◽  
Fluorescent Dye ◽  
Proximal Tubules ◽  
Acid Base ◽  
Rate Of Recovery

In nonperfused proximal tubules isolated from chicken long-looped mammalian-type nephrons, intracellular pH (pHi), measured with the pH-sensitive fluorescent dye 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein, was ∼7.3 under control conditions (HEPES-buffered medium with pH 7.4 at 37°C) and was reduced to ∼7.0 in response to NH4Cl pulse. The rate of recovery of pHi from this level to the resting level was 1) significantly reduced by the removal of Na+ from the bath, 2) significantly increased by the removal of Cl− from the bath, 3) unchanged by the removal of both Na+ and Cl− from the bath, 4) significantly reduced by the addition of either ethylisopropylamiloride or DIDS to the bath, 5) significantly increased by a high bath K+ concentration, and 6) unchanged by the addition of Ba2+ to the bath. These data suggest that both Na+-coupled and Cl−-coupled basolateral acid-base fluxes are involved in determining the rate of recovery of pHi after acidification. The most likely ones to be important in regulating pHi are a Na+/H+exchanger and a Na+-coupled Cl−/[Formula: see text]exchanger. In birds, long-looped mammalian-type nephrons resemble short-looped transitional nephrons but differ markedly from superficial loopless reptilian-type nephrons.

Multifunctional gold nanoparticles as smart nanovehicles with enhanced tumour-targeting abilities for intracellular pH mapping and in vivo MR/fluorescence imaging

Nanoscale ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 2002-2010 ◽  
Author(s):  
Kang-Kang Yu ◽  
Kun Li ◽  
Chun-Yan Lu ◽  
Yong-Mei Xie ◽  
Yan-Hong Liu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Imaging ◽  
Intracellular Ph ◽  
Living Cells ◽  
Tumour Targeting ◽  
Tumour Imaging ◽  
Pathological Changes

A number of multimodal agents have been developed for tumour imaging and diagnosis, but most of them cannot be used to study the detailed physiological or pathological changes in living cells at the same time.

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