Construction of pH-Sensitive “Submarine” Based on Gold Nanoparticles with Double Insurance for Intracellular pH Mapping, Quantifying of Whole Cells and in Vivo Applications

2016 ◽  
Vol 8 (35) ◽  
pp. 22839-22848 ◽  
Author(s):  
Kang-Kang Yu ◽  
Kun Li ◽  
Hui-Huan Qin ◽  
Qian Zhou ◽  
Chen-Hui Qian ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Intracellular Ph ◽  
Ph Sensitive ◽  
Whole Cells

Multifunctional gold nanoparticles as smart nanovehicles with enhanced tumour-targeting abilities for intracellular pH mapping and in vivo MR/fluorescence imaging

Nanoscale ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 2002-2010 ◽  
Author(s):  
Kang-Kang Yu ◽  
Kun Li ◽  
Chun-Yan Lu ◽  
Yong-Mei Xie ◽  
Yan-Hong Liu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Imaging ◽  
Intracellular Ph ◽  
Living Cells ◽  
Tumour Targeting ◽  
Tumour Imaging ◽  
Pathological Changes

A number of multimodal agents have been developed for tumour imaging and diagnosis, but most of them cannot be used to study the detailed physiological or pathological changes in living cells at the same time.

scholarly journals Natural tripeptide capped pH-sensitive gold nanoparticles for efficacious doxorubicin delivery both in vitro and in vivo

Nanoscale ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 1067-1074 ◽  
Author(s):  
Krishan Kumar ◽  
Parikshit Moitra ◽  
Mohsin Bashir ◽  
Paturu Kondaiah ◽  
Santanu Bhattacharya
Keyword(s):  
Gold Nanoparticles ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Ph Sensitive ◽  
Drug Resistant ◽  
Ph Responsive ◽  
Line P

AuNPs capped with a natural pH-responsive short tripeptide sequence are presented herein for the successful delivery of doxorubicin both in vitro and in vivo. Efficacy of the system has also been evaluated in drug resistant cancer cell line.

Raman/fluorescence dual-sensing and imaging of intracellular pH distribution

2015 ◽  
Vol 51 (99) ◽  
pp. 17584-17587 ◽  
Author(s):  
Yue Cao ◽  
Ruo-Can Qian ◽  
Da-Wei Li ◽  
Yi-Tao Long
Keyword(s):  
Gold Nanoparticles ◽  
Intracellular Ph ◽  
Ph Sensitive ◽  
Ph Distribution ◽  
Dual Imaging ◽  
Dual Sensing

A pH-sensitive probe has been designed based on DNA modified gold nanoparticles (AuNPs) for Raman/fluorescence dual-imaging of the intracellular pH distribution.

scholarly journals In vivo measurement of cytosolic and mitochondrial pH using a pH-sensitive GFP derivative in Saccharomyces cerevisiae reveals a relation between intracellular pH and growth

Microbiology ◽  
2009 ◽  
Vol 155 (1) ◽  
pp. 268-278 ◽  
Author(s):  
Rick Orij ◽  
Jarne Postmus ◽  
Alex Ter Beek ◽  
Stanley Brul ◽  
Gertien J. Smits
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Intracellular Ph ◽  
Respiratory Chain ◽  
Sorbic Acid ◽  
Accurate Determination ◽  
Acid Stress ◽  
Ph Sensitive ◽  
Cytosolic Ph ◽  
Constant Ph

The specific pH values of cellular compartments affect virtually all biochemical processes, including enzyme activity, protein folding and redox state. Accurate, sensitive and compartment-specific measurements of intracellular pH (pHi) dynamics in living cells are therefore crucial to the understanding of stress response and adaptation. We used the pH-sensitive GFP derivative ‘ratiometric pHluorin’ expressed in the cytosol and in the mitochondrial matrix of growing Saccharomyces cerevisiae to assess the variation in cytosolic pH (pHcyt) and mitochondrial pH (pHmit) in response to nutrient availability, respiratory chain activity, shifts in environmental pH and stress induced by addition of sorbic acid. The in vivo measurement allowed accurate determination of organelle-specific pH, determining a constant pHcyt of 7.2 and a constant pHmit of 7.5 in cells exponentially growing on glucose. We show that pHcyt and pHmit are differentially regulated by carbon source and respiratory chain inhibitors. Upon glucose starvation or sorbic acid stress, pHi decrease coincided with growth stasis. Additionally, pHi and growth coincided similarly in recovery after addition of glucose to glucose-starved cultures or after recovery from a sorbic acid pulse. We suggest a relation between pHi and cellular energy generation, and therefore a relation between pHi and growth.

Dosimetry and Radioenhancement Comparison of Gold Nanoparticles in Kilovoltage and Megavoltage Radiotherapy using MAGAT Polymer Gel Dosimeter

2019 ◽  
Vol 9 (2Apr) ◽  
Author(s):  
S Farahani ◽  
N Riyahi Alam ◽  
S Haghgoo ◽  
M Khoobi ◽  
Gh Geraily ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
3D Visualization ◽  
Energy Levels ◽  
Polymer Gel ◽  
External Radiotherapy ◽  
Dose Enhancement ◽  
Internal Radiotherapy ◽  
Boost Radiotherapy

Background: Numerous unique characteristics of the nanosized gold, including high atomic number, low toxicity, and high biocompatibility make it one of the most appropriate nanostructures to boost radiotherapy efficacy. Many in-vivo and in-vitro investigations have indicated that gold nanoparticles (AuNPs) can significantly increase tumor injuries in low kilovoltage radiotherapy. While deep-lying tumors require much higher energy levels with greater penetration power, and investigations carried out in megavoltage energy range show contradictory results.Objective: In this study, we quantitatively assess and compare dose enhancement factors (DEFs) obtained through AuNPs under radiation of Cobalt-60 source (1.25MeV) versus Iridium-192 source (0.380 KeV) using MAGAT gel dosimeter.Material and Methods: MAGAT polymer gel in both pure and combined with 0.2 mM AuNPs was synthesized. In order to quantify the effect of energy on DEF, irradiation was carried out by Co-60 external radiotherapy and Ir-192 internal radiotherapy. Finally, readings of irradiated and non-irradiated gels were performed by MR imaging.Result: The radiation-induced R2 (1/T2) changes of the gel tubes doped with AuNPs compared to control samples, upon irradiation of beams released by Ir-192 source showed a significant dose enhancement (15.31% ±0.30) relative to the Co-60 external radiotherapy (5.85% ±0.14).Conclusion: This preliminary study suggests the feasibility of using AuNPs in radiation therapy (RT), especially in low-energy sources of brachytherapy. In addition, MAGAT polymer gel, as a powerful dosimeter, could be used for 3D visualization of radiation dose distribution of AuNPs in radiotherapy.

Antitumor Effect of Zwitterions of Imidazolium Derived from L-methionine in BALB/c Mice with Lymphoma L5178Y

2020 ◽  
Vol 17 (1) ◽  
pp. 33-39
Author(s):  
Karen C. Vargas-Castro ◽  
Ana M. Puebla Pérez ◽  
Irma I. Rangel-Salas ◽  
Jorge I. Delgado-Saucedo ◽  
José B. Pelayo-Vázquez ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Antitumor Activity ◽  
Antitumor Agent ◽  
Inhibitory Effect ◽  
Stabilizing Agent ◽  
Biological Compatibility ◽  
In Vivo Tests ◽  
Antitumoral Agent ◽  
Group 1

Background: In the therapy of cancer, several treatments have been designed using nanomaterials, among which gold nanoparticles (AuNPs) have been featured as a promising antitumoral agent. Our research group has developed the synthesis of gold nanoparticles L-AuNPs and D-AuNPs stabilized with zwitterions of imidazolium (L-1 and D-1) derived from L-methionine and D-methionine. Because the stabilizer agent is chiral, we observed through circular dichroism that AuNPs also present chirality; such chirality as well as the fact that the stabilizing agent contains fragments of methionine and imidazolium that are commonly involved in biological processes, opens up the possibility that this system may have biological compatibility. Additionally, the presence of methionine in the stabilizing agent opens the application of this system as a possible antitumor agent because methionine is involved in methylation processes of molecules such as DNA. Objective: The aim of this research is the evaluation of the antitumor activity of gold nanoparticles stabilized with zwitterions of imidazolium (L-AuNPs) derived from L-methionine in the model of BALB/c mice with lymphoma L5178Y. Methods: Taking as a parameter cell density, the evaluation of the inhibitory effect of L-AuNPs was carried out with a series of in vivo tests in BALB/c type mice; three groups of five mice each were formed (Groups 1, 2 and 3); all mice were i.p. inoculated with the lymphoblast murine L5178Y. Group 1 consisted of mice without treatment. In the Groups 2 and 3 the mice were treated with L-AuNPs at 0.3 mg/Kg on days 1, 7 and 14 by orally and intraperitonally respectively. Results: These results show low antitumor activity of these gold nanoparticles (L-NPsAu) but interestingly, the imidazolium stabilizing agent of gold nanoparticle (L-1) displayed promising antitumor activity. On the other hand, the enantiomer of L-1, (D-1) as well as asymmetric imidazole derivate from L-methionine (L-2), do not exhibit the same activity as L-1. Conclusion: The imidazolium stabilizing agent (L-1) displayed promising antitumor activity. Modifications in the structure of L-1 showed that, the stereochemistry (like D-1) and the presence of methionine fragments (like L-2) are determinants in the antitumor activity of this compound.

scholarly journals Radiosensitization of Prostate Cancers In Vitro and In Vivo to Erbium-filtered Orthovoltage X-rays Using Actively Targeted Gold Nanoparticles

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Allison M. Khoo ◽  
Sang Hyun Cho ◽  
Francisco J. Reynoso ◽  
Maureen Aliru ◽  
Kathryn Aziz ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
X Rays ◽  
Prostate Cancers
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