Azeotropic drying free [18F]FDG synthesis and its application to a lab-on-chip platform

2016 ◽  
Vol 52 (4) ◽  
pp. 729-732 ◽  
Author(s):  
S. Lindner ◽  
C. Rensch ◽  
S. Neubaur ◽  
M. Neumeier ◽  
R. Salvamoser ◽  
...  
Keyword(s):  
Proof Of Concept ◽  
Hardware Complexity ◽  
Lab On Chip ◽  
18F Fdg ◽  
On Chip

[18F]FDG was prepared using a cartridge-based drying technique for [18F]fluoride. The application to a lab-on-chip platform demonstrates a proof of concept towards reduced hardware complexity.

scholarly journals Proof-of-concept demonstration of free-form optics enhanced confocal Raman spectroscopy in combination with optofluidic lab-on-chip

2016 ◽  
Author(s):  
Qing Liu ◽  
Diane De Coster ◽  
Damien Loterie ◽  
Jürgen Van Erps ◽  
Michael Vervaeke ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Free Form ◽  
Proof Of Concept ◽  
Confocal Raman Spectroscopy ◽  
Lab On Chip ◽  
Confocal Raman ◽  
On Chip

scholarly journals A Proof-of-Concept Study Using Numerical Simulations of an Acoustic Spheroid-on-a-Chip Platform for Improving 3D Cell Culture

Sensors ◽  
2021 ◽  
Vol 21 (16) ◽  
pp. 5529
Author(s):  
Arash Yahyazadeh Shourabi ◽  
Roozbeh Salajeghe ◽  
Maryam Barisam ◽  
Navid Kashaninejad
Keyword(s):  
Flow Rate ◽  
Cultured Cells ◽  
3D Cell Culture ◽  
Potential Candidate ◽  
Proof Of Concept ◽  
Multicellular Spheroids ◽  
Lab On Chip ◽  
Main Challenge ◽  
Biological Studies ◽  
On Chip

Microfluidic lab-on-chip devices are widely being developed for chemical and biological studies. One of the most commonly used types of these chips is perfusion microwells for culturing multicellular spheroids. The main challenge in such systems is the formation of substantial necrotic and quiescent zones within the cultured spheroids. Herein, we propose a novel acoustofluidic integrated platform to tackle this bottleneck problem. It will be shown numerically that such an approach is a potential candidate to be implemented to enhance cell viability and shrinks necrotic and quiescent zones without the need to increase the flow rate, leading to a significant reduction in costly reagents’ consumption in conventional spheroid-on-a-chip platforms. Proof-of-concept, designing procedures and numerical simulation are discussed in detail. Additionally, the effects of acoustic and hydrodynamic parameters on the cultured cells are investigated. The results show that by increasing acoustic boundary displacement amplitude (d0), the spheroid’s proliferating zone enlarges greatly. Moreover, it is shown that by implementing d0  = 0.5 nm, the required flow rate to maintain the necrotic zone below 13% will be decreased 12 times compared to non-acoustic chips.

scholarly journals An Acoustic Spheroid-on-Chip Platform for Long-Term Culturing of 3D Cell Aggregates

2021 ◽  
Author(s):  
Arash Yahyazadeh Shourabi ◽  
Roozbeh Salajeghe ◽  
Maryam Barisam ◽  
Navid Kashaninejad
Keyword(s):  
Flow Rate ◽  
Cultured Cells ◽  
Proof Of Concept ◽  
Multicellular Spheroids ◽  
Lab On Chip ◽  
Main Challenge ◽  
Biological Studies ◽  
Finite Element Numerical Simulation ◽  
On Chip

Microfluidic lab-on-chip devices are widely being developed for chemical and biological studies. One of the most commonly used types of these chips is perfusion microwells for culturing multicellular spheroids. The main challenge in such systems is the formation of substantial necrotic and hypoxic zones within the cultured spheroids. Herein, we propose a novel acoustofluidic integrated platform to tackle this bottleneck problem. We show that such an approach enhances cell viability and shrinks necrotic and hypoxic zones in these spheroid-on-a-chip platforms without the need to increase the flow rate, leading to a significant reduction in costly reagents' consumption. Proof-of-concept, designing procedures, and finite element numerical simulation are discussed in details. Also, the effects of acoustic and hydrodynamic parameters on the cultured cells are investigated. The results show that by increasing acoustic boundary displacement amplitude (d0), the spheroid’s proliferating zone enlarges greatly. Moreover, it is shown that by implementing d0=0.5 nm, the required flow rate to maintain the necrotic zone below 13% will be decreased 12 times compared to non-acoustic chips.

scholarly journals Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kanako Saito ◽  
Yuri Ota ◽  
Dieter M. Tourlousse ◽  
Satoko Matsukura ◽  
Hirotsugu Fujitani ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Purity ◽  
Droplet Microfluidics ◽  
Individual Growth ◽  
Proof Of Concept ◽  
Widespread Adoption ◽  
Technical Challenges ◽  
On Chip ◽  
Uncultured Microorganisms ◽  
Environmental Microbes

AbstractDroplet microfluidics has emerged as a powerful technology for improving the culturing efficiency of environmental microorganisms. However, its widespread adoption has been limited due to considerable technical challenges, especially related to identification and manipulation of individual growth-positive droplets. Here, we combined microfluidic droplet technology with on-chip “fluorescent nucleic acid probe in droplets for bacterial sorting” (FNAP-sort) for recovery of growth-positive droplets and droplet microdispensing to establish an end-to-end workflow for isolation and culturing of environmental microbes. As a proof-of-concept, we demonstrate the ability of our technique to yield high-purity cultures of rare microorganisms from a representative complex environmental microbiome. As our system employs off-the-shelf commercially available equipment, we believe that it can be readily adopted by others and may thus find widespread use toward culturing the high proportion of as-of-yet uncultured microorganisms in different biomes.

scholarly journals A Lab‐On‐chip Tool for Rapid, Quantitative, and Stage‐selective Diagnosis of Malaria

2021 ◽  
pp. 2004101
Author(s):  
Marco Giacometti ◽  
Francesca Milesi ◽  
Pietro Lorenzo Coppadoro ◽  
Alberto Rizzo ◽  
Federico Fagiani ◽  
...  
Keyword(s):  
Lab On Chip ◽  
On Chip

scholarly journals Integrated Magnetohydrodynamic Pump with Magnetic Composite Substrate and Laser-Induced Graphene Electrodes

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1113
Author(s):  
Mohammed Asadullah Khan ◽  
Jürgen Kosel
Keyword(s):  
Magnetic Flux ◽  
Flow Rate ◽  
Magnetic Composite ◽  
Propulsive Force ◽  
Closed Channel ◽  
Lab On Chip ◽  
Composite Substrate ◽  
On Chip ◽  
High Level ◽  
Moving Parts

An integrated polymer-based magnetohydrodynamic (MHD) pump that can actuate saline fluids in closed-channel devices is presented. MHD pumps are attractive for lab-on-chip applications, due to their ability to provide high propulsive force without any moving parts. Unlike other MHD devices, a high level of integration is demonstrated by incorporating both laser-induced graphene (LIG) electrodes as well as a NdFeB magnetic-flux source in the NdFeB-polydimethylsiloxane permanent magnetic composite substrate. The effects of transferring the LIG film from polyimide to the magnetic composite substrate were studied. Operation of the integrated magneto hydrodynamic pump without disruptive bubbles was achieved. In the studied case, the pump produces a flow rate of 28.1 µL/min. while consuming ~1 mW power.

scholarly journals Towards lab-on-chip ultrasensitive ethanol detection using photonic crystal waveguide operating in the mid infrared

Nanophotonics ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ali Rostamian ◽  
Ehsan Madadi-Kandjani ◽  
Hamed Dalir ◽  
Volker J. Sorger ◽  
Ray T. Chen
Keyword(s):  
Photonic Crystal ◽  
Slow Light ◽  
High Sensitivity ◽  
Guided Wave ◽  
Silicon On Insulator ◽  
Group Index ◽  
Photonic Crystal Waveguide ◽  
Mid Infrared ◽  
Lab On Chip ◽  
On Chip

Abstract Thanks to the unique molecular fingerprints in the mid-infrared spectral region, absorption spectroscopy in this regime has attracted widespread attention in recent years. Contrary to commercially available infrared spectrometers, which are limited by being bulky and cost-intensive, laboratory-on-chip infrared spectrometers can offer sensor advancements including raw sensing performance in addition to use such as enhanced portability. Several platforms have been proposed in the past for on-chip ethanol detection. However, selective sensing with high sensitivity at room temperature has remained a challenge. Here, we experimentally demonstrate an on-chip ethyl alcohol sensor based on a holey photonic crystal waveguide on silicon on insulator-based photonics sensing platform offering an enhanced photoabsorption thus improving sensitivity. This is achieved by designing and engineering an optical slow-light mode with a high group-index of n g  = 73 and a strong localization of modal power in analyte, enabled by the photonic crystal waveguide structure. This approach includes a codesign paradigm that uniquely features an increased effective path length traversed by the guided wave through the to-be-sensed gas analyte. This PIC-based lab-on-chip sensor is exemplary, spectrally designed to operate at the center wavelength of 3.4 μm to match the peak absorbance for ethanol. However, the slow-light enhancement concept is universal offering to cover a wide design-window and spectral ranges towards sensing a plurality of gas species. Using the holey photonic crystal waveguide, we demonstrate the capability of achieving parts per billion levels of gas detection precision. High sensitivity combined with tailorable spectral range along with a compact form-factor enables a new class of portable photonic sensor platforms when combined with integrated with quantum cascade laser and detectors.

Monitoring microbial growth on a microfluidic lab-on-chip with electrochemical impedance spectroscopic technique

2021 ◽  
Vol 23 (2) ◽  
Author(s):  
Subhan Shaik ◽  
Aarthi Saminathan ◽  
Deepak Sharma ◽  
Jagdish A Krishnaswamy ◽  
D Roy Mahapatra
Keyword(s):  
Microbial Growth ◽  
Electrochemical Impedance ◽  
Spectroscopic Technique ◽  
Lab On Chip ◽  
On Chip ◽  
Electrochemical Impedance Spectroscopic

scholarly journals Design Methodology of Passive In-Line Relays for Molecular Communication in Flow-Induced Microfluidic Channel

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 65
Author(s):  
Puneet Manocha ◽  
Gitanjali Chandwani
Keyword(s):  
Communication System ◽  
Communication Systems ◽  
Microfluidic Channel ◽  
Microfluidic Channels ◽  
Molecular Communication ◽  
External Energy ◽  
Lab On Chip ◽  
Molecular Communication Systems ◽  
Macro Scale ◽  
On Chip

Molecular communication is a bioinspired communication that enables macro-scale, micro-scale and nano-scale devices to communicate with each other. The molecular communication system is prone to severe signal attenuation, dispersion and delay, which leads to performance degradation as the distance between two communicating devices increases. To mitigate these challenges, relays are used to establish reliable communication in microfluidic channels. Relay assisted molecular communication systems can also enable interconnection among various entities of the lab-on-chip for sharing information. Various relaying schemes have been proposed for reliable molecular communication systems, most of which lack practical feasibility. Thus, it is essential to design and develop relays that can be practically incorporated into the microfluidic channel. This paper presents a novel design of passive in-line relay for molecular communication system that can be easily embedded in the microfluidic channel and operate without external energy. Results show that geometric modification in the microfluidic channel can act as a relay and restore the degraded signal up-to 28%.

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