A polydopamine nanosphere based highly sensitive and selective aptamer cytosensor with enzyme amplification

2016 ◽  
Vol 52 (2) ◽  
pp. 406-409 ◽  
Author(s):  
Daoqing Fan ◽  
Changtong Wu ◽  
Kun Wang ◽  
Xiaoxiao Gu ◽  
Yaqing Liu ◽  
...  
Keyword(s):  
Highly Sensitive ◽  
Model Cell ◽  
Enzyme Amplification ◽  
First Time

With CCRF-CEM as the model cell, a highly sensitive and selective cytosensor was developed by taking advantage of polydopamine nanospheres for the first time.

Intramolecular photoinitiator induced atom transfer radical polymerization for electrochemical DNA detection

The Analyst ◽  
2020 ◽  
Vol 145 (3) ◽  
pp. 858-864
Author(s):  
Ligang Ma ◽  
Qianrui Liu ◽  
Lihe Jian ◽  
Shan Ye ◽  
Xiaoke Zheng ◽  
...  
Keyword(s):  
Atom Transfer Radical Polymerization ◽  
Radical Polymerization ◽  
Electrochemical Biosensor ◽  
Dna Detection ◽  
Atom Transfer ◽  
Highly Sensitive ◽  
Electrochemical Dna Detection ◽  
First Time

A novel electrochemical biosensor was reported for the first time to achieve highly sensitive DNA detection based on photoinduced atom transfer radical polymerization (photoATRP).

scholarly journals Analysis of free and protein-bound nitrotyrosine in human plasma by a gas chromatography/mass spectrometry method that avoids nitration artifacts

2000 ◽  
Vol 345 (3) ◽  
pp. 453-458 ◽  
Author(s):  
Matthew T. FROST ◽  
Barry HALLIWELL ◽  
Kevin P. MOORE
Keyword(s):  
Reactive Nitrogen Species ◽  
Plasma Proteins ◽  
Biological Fluids ◽  
Plasma Concentrations ◽  
Normal Subjects ◽  
Gas Chromatography Mass Spectrometry ◽  
Highly Sensitive ◽  
Acidic Conditions ◽  
First Time

Measurement of nitrotyrosine in biological fluids and tissues is increasingly being used to monitor the production of reactive nitrogen species in vivo. The detection of nitrotyrosine in vivo has been reported with the use of a variety of methods including immunoassay, HPLC and GLC/MS. The validity of HPLC and immunoassays have been questioned with regard to their selectivity and sensitivity limits. In principle, the measurement of nitrotyrosine by GLC/MS permits a highly specific, highly sensitive and fully quantitative assay. The nitration of tyrosine under acidic conditions in the presence of nitrite is well documented. Derivatization for the full quantification of nitrotyrosine by using GLC/MS can lead to the artifactual nitration of tyrosine if performed under acidic conditions in the presence of nitrite. We describe a novel alkaline method for the hydrolysis and derivatization of nitrotyrosine and tyrosine, and demonstrate its applicability to the measurement of plasma concentrations of both free and protein-bound nitrotyrosine and tyrosine. A detection limit of 1 pg for nitrotyrosine and 100 pg for tyrosine has been achieved. Our method allows, for the first time, the analysis of free and protein-bound nitrotyrosine and tyrosine in biological samples. The plasma concentrations (means±S.E.M.) of free tyrosine and nitrotyrosine in eight normal subjects were 12±0.6 μg/ml and 14±0.7 ng/ml respectively. Plasma proteins contained tyrosine and nitrotyrosine at 60.7±1.7 μg/mg and 2.7±0.4 ng/mg respectively.

scholarly journals The growth of interest for astronomical X-ray polarimetry

2018 ◽  
Author(s):  
Frédéric Marin
Keyword(s):  
Quantitative Assessment ◽  
Supernova Remnant ◽  
Scientific Literature ◽  
High Energy ◽  
X Ray ◽  
Highly Sensitive ◽  
In The Beginning ◽  
First Time

Astronomical X-ray polarimetry was first explored in the end of the 60's by pioneering rocket instruments. The craze arising from the first discoveries on stellar and supernova remnant X-ray polarization led to the addition of X-ray polarimeters on-board of early satellites. Unfortunately, the inadequacy of the diffraction and scattering technologies required to measure polarization with respect to the constraints driven by X-ray mirrors and detectors, coupled to long integration times, slowed down the field for almost 40 years. Thanks to the development of new, highly sensitive, compact X-ray polarimeters in the beginning of the 2000's, the possibility to observe astronomical X-ray polarization is rising again and scientists are now ready to explore the high energy sky thanks to modern X-ray polarimeters. In the forthcoming years, several X-ray missions (both rockets, balloons and satellites) will open a new observational windows. A wind of renewal blows over the area of X-ray polarimetry and this paper presents for the first time a quantitative assessment, all based on scientific literature, of the growth of interest for astronomical X-ray polarimetry.

scholarly journals Highly sensitive p-type 4H-SiC van der Pauw sensor

RSC Advances ◽  
2018 ◽  
Vol 8 (6) ◽  
pp. 3009-3013 ◽  
Author(s):  
Tuan-Khoa Nguyen ◽  
Hoang-Phuong Phan ◽  
Jisheng Han ◽  
Toan Dinh ◽  
Abu Riduan Md Foisal ◽  
...  
Keyword(s):  
Silicon Carbide ◽  
Strain Sensor ◽  
Highly Sensitive ◽  
Van Der Pauw ◽  
P Type ◽  
First Time

This paper presents for the first time a p-type 4H silicon carbide (4H-SiC) van der Pauw strain sensor by utilizing the strain induced effect in four-terminal devices.

Revealing the unseen: the organizer region of the nucleolus

2001 ◽  
Vol 114 (17) ◽  
pp. 3199-3205 ◽  
Author(s):  
Marco Biggiogera ◽  
Manuela Malatesta ◽  
Sousan Abolhassani-Dadras ◽  
François Amalric ◽  
Lawrence I. Rothblum ◽  
...  
Keyword(s):  
Inner Core ◽  
Organizer Region ◽  
Exact Distribution ◽  
Dense Fibrillar Component ◽  
Fibrillar Center ◽  
Energy Filtering ◽  
Dna And Rna ◽  
Highly Sensitive ◽  
Fibrillar Component ◽  
First Time

We carried out a high-resolution ultrastructural analysis of the nucleolus in mouse P815 cells by combining specific DNA and RNA staining, anti-fibrillarin immunolabeling, contrast enhancement by energy filtering TEM and phosphorus mapping by ESI to visualize nucleic acids. We demonstrated that specifically contrasted DNA, fibrillarin and phosphorus overlap within the nucleolar dense fibrillar component. Moreover, we describe a ‘DNA cloud’ consisting of an inner core of DNA fibers (fibrillar center) and a periphery made of extremely thin fibrils overlapping the anti-fibrillarin immunolabeling (dense fibrillar component). This highly sensitive approach has allowed us to demonstrate, for the first time, the exact distribution of DNA within the decondensed interphase counterpart of the NOR, which includes both the fibrillar center and the dense fibrillar component.

scholarly journals Highly Sensitive and Selective Fluorescent Detection of Gossypol Based on BSA-Stabilized Copper Nanoclusters

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 95 ◽  
Author(s):  
Shuangjiao Xu ◽  
Kehai Zhou ◽  
Dan Fang ◽  
Lei Ma
Keyword(s):  
High Performance ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Hplc Method ◽  
Fluorescent Detection ◽  
Copper Nanoclusters ◽  
Highly Sensitive ◽  
The Us ◽  
Fluorescence Quenching Mechanism ◽  
First Time

In this paper, fluorescent copper nanoclusters (NCs) are used as a novel probe for the sensitive detection of gossypol for the first time. Based on a fluorescence quenching mechanism induced by interactions between bovine serum albumin (BSA) and gossypol, fluorescent BSA-Cu NCs were seen to exhibit a high sensitivity to gossypol in the range of 0.1–100 µM. The detection limit for gossypol is 25 nM at a signal-to-noise ratio of three, which is approximately 35 times lower than the acceptable limit (0.9 µM) defined by the US Food and Drug Administration for cottonseed products. Moreover, the proposed method for gossypol displays excellent selectivity over many common interfering species. We also demonstrate the application of the present method to the measurement of several real samples with satisfactory recoveries, and the results agree well with those obtained using the high-performance liquid chromatography (HPLC) method. The method based on Cu NCs offers the followings advantages: simplicity of design, facile preparation of nanomaterials, and low experimental cost.

Highly sensitive and selective non-enzymatic glucose detection based on indigo carmine/hemin/H2O2 chemiluminescence

The Analyst ◽  
2020 ◽  
Vol 145 (3) ◽  
pp. 1041-1046 ◽  
Author(s):  
Tadesse Haile Fereja ◽  
Shimeles Addisu Kitte ◽  
Muhammad Nadeem Zafar ◽  
Mohamed Ibrahim Halawa ◽  
Shuang Han ◽  
...  
Keyword(s):  
Indigo Carmine ◽  
Glucose Detection ◽  
Highly Sensitive ◽  
Enzymatic Detection ◽  
First Time

Chemiluminescence of indigo carmine/glucose/hemin/H2O2 has been reported for the first time, and used for sensitive non-enzymatic detection of glucose and indigo carmine.

scholarly journals Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

2020 ◽  
Vol 21 (20) ◽  
pp. 7465 ◽  
Author(s):  
Vasilisa V. Krasitskaya ◽  
Eugenia E. Bashmakova ◽  
Ludmila A. Frank
Keyword(s):  
Analytical Methods ◽  
Analytical Tool ◽  
Biological Research ◽  
Research Areas ◽  
Enzyme Substrate ◽  
Highly Sensitive ◽  
Powerful Analytical Tool ◽  
Fundamental Research ◽  
Common Group ◽  
First Time

The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by luciferase. Despite the diversity in structures and the functioning mechanisms, these enzymes can be united into a common group called CTZ-dependent luciferases. Among these, there are two sharply different types of the system organization—Ca2+-regulated photoproteins and luciferases themselves that function in accordance with the classical enzyme–substrate kinetics. Along with deep and comprehensive fundamental research on these systems, approaches and methods of their practical use as highly sensitive reporters in analytics have been developed. The research aiming at the creation of artificial luciferases and synthetic CTZ analogues with new unique properties has led to the development of new experimental analytical methods based on them. The commercial availability of many ready-to-use assay systems based on CTZ-dependent luciferases is also important when choosing them by first-time-users. The development of analytical methods based on these bioluminescent systems is currently booming. The bioluminescent systems under consideration were successfully applied in various biological research areas, which confirms them to be a powerful analytical tool. In this review, we consider the main directions, results, and achievements in research involving these luciferases.

A H2O2-free electrochemical peptide biosensor based on Au@Pt bimetallic nanorods for highly sensitive sensing of matrix metalloproteinase 2

2020 ◽  
Vol 56 (45) ◽  
pp. 6039-6042 ◽  
Author(s):  
Xiaoxue Xi ◽  
Meiqi Wen ◽  
Shihao Song ◽  
Junlun Zhu ◽  
Wei Wen ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Sensitive Detection ◽  
Matrix Metalloproteinase 2 ◽  
Highly Sensitive ◽  
Bimetallic Nanorods ◽  
Highly Sensitive Detection ◽  
First Time

We construct for the first time a H2O2-free electrochemical peptide biosensor based on Au@Pt bimetallic nanorods in neutral substrate solutions for highly sensitive detection of matrix metalloproteinase 2.

A highly sensitive and label-free electrochemiluminescence immunosensor for beta 2-microglobulin

2017 ◽  
Vol 9 (17) ◽  
pp. 2570-2577 ◽  
Author(s):  
Mohammad Rizwan ◽  
Noor Faizah Mohd-Naim ◽  
Natasha Ann Keasberry ◽  
Minhaz Uddin Ahmed
Keyword(s):  
Quantum Dot ◽  
Cadmium Selenide ◽  
Gold Nanoparticle ◽  
Label Free ◽  
Screen Printed Electrode ◽  
Highly Sensitive ◽  
Beta 2 ◽  
Beta 2 Microglobulin ◽  
First Time ◽  
Screen Printed

A highly sensitive and label-free electrochemiluminescence (ECL) immunosensor was fabricated for the detection of β-2-microglobulin (β2M) based on a gold nanoparticle-doped@carbon nano-onion chitosan nanocomposite modified cadmium selenide quantum dot screen-printed electrode (QDs-SPE/AuNPs@CNOs-CS) for the first time.

Sign in / Sign up

Export Citation Format

Share Document