In cellulo protein labelling with Ru-conjugate for luminescence imaging and bioorthogonal photocatalysis

2015 ◽  
Vol 51 (93) ◽  
pp. 16664-16666 ◽  
Author(s):  
Kalyan K. Sadhu ◽  
E. Lindberg ◽  
N. Winssinger
Keyword(s):  
Ruthenium Complex ◽  
Photocatalytic Reduction ◽  
Live Cells ◽  
Direct Visualization ◽  
Protein Labelling ◽  
Aryl Azide ◽  
Luminescence Imaging

Labelling of proteins with a luminescent ruthenium complex enables the direct visualization and photocatalytic reduction of aryl azide in live cells.

Development of organelle-targetable europium complex probes for time-gated luminescence imaging of hypochlorous acid in live cells and animals

2017 ◽  
Vol 140 ◽  
pp. 407-416 ◽  
Author(s):  
Hua Ma ◽  
Bo Song ◽  
Yuanxiu Wang ◽  
Chaolong Liu ◽  
Xin Wang ◽  
...  
Keyword(s):  
Hypochlorous Acid ◽  
Europium Complex ◽  
Live Cells ◽  
Luminescence Imaging

Visible light assisted photocatalytic reduction of CO2 using a graphene oxide supported heteroleptic ruthenium complex

2015 ◽  
Vol 17 (3) ◽  
pp. 1605-1609 ◽  
Author(s):  
Pawan Kumar ◽  
Amit Bansiwal ◽  
Nitin Labhsetwar ◽  
Suman L. Jain
Keyword(s):  
Graphene Oxide ◽  
Visible Light ◽  
Ruthenium Complex ◽  
Photocatalytic Reduction ◽  
Covalent Attachment ◽  
Microwave Technique ◽  
Reduction Of Co2

A new heteroleptic ruthenium complex containing 2-thiophenyl benzimidazole ligands was synthesized using a microwave technique and was immobilized to graphene oxide via covalent attachment.

Reaction-based phosphorescent nanosensor for ratiometric and time-resolved luminescence imaging of fluoride in live cells

2015 ◽  
Vol 51 (64) ◽  
pp. 12839-12842 ◽  
Author(s):  
Shujuan Liu ◽  
Jie Zhang ◽  
Danfeng Shen ◽  
Hua Liang ◽  
Xiangmei Liu ◽  
...  
Keyword(s):  
Live Cells ◽  
Time Resolved ◽  
Luminescence Imaging

A two-channel phosphorescent nanosensor has been designed for use in ratiometric and time-resolved luminescence imaging of intracellular F−.

A Ni-NTA-based red fluorescence probe for protein labelling in live cells

2017 ◽  
Vol 5 (6) ◽  
pp. 1166-1173 ◽  
Author(s):  
Ailun Chao ◽  
Nan Jiang ◽  
Ya Yang ◽  
Hongyan Li ◽  
Hongzhe Sun
Keyword(s):  
Protein Purification ◽  
Fluorescence Probe ◽  
Live Cells ◽  
Great Success ◽  
Protein Labelling ◽  
Red Fluorescence

The great success of a His6-Ni-nitrilotriaceate (Ni-NTA) system in protein purification has inspired scientists to develop novel Ni-NTA based fluoresent probes for imaging of proteins in live cells.

Direct visualization of nucleolar G-quadruplexes in live cells by using a fluorescent light-up probe

2018 ◽  
Vol 1862 (5) ◽  
pp. 1101-1106 ◽  
Author(s):  
Suge Zhang ◽  
Hongxia Sun ◽  
Hongbo Chen ◽  
Qian Li ◽  
Aijiao Guan ◽  
...  
Keyword(s):  
Live Cells ◽  
Fluorescent Light ◽  
Direct Visualization

Direct visualization of repair of oxidative damage by OGG1 in the nuclei of live cells

2010 ◽  
Vol 25 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Agnieszka Zielinska ◽  
Owain T. Davies ◽  
Rosalind A. Meldrum ◽  
Nikolas J. Hodges
Keyword(s):  
Oxidative Damage ◽  
Live Cells ◽  
Direct Visualization

scholarly journals Direct Visualization of Amlodipine Intervention into Living Cells by Means of Fluorescence Microscopy

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2997
Author(s):  
Christine Quentin ◽  
Rūta Gerasimaitė ◽  
Alexandra Freidzon ◽  
Levon S. Atabekyan ◽  
Gražvydas Lukinavičius ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Aqueous Solutions ◽  
Time Lapse ◽  
Drug Uptake ◽  
Live Cells ◽  
Direct Visualization ◽  
Cellular Environment ◽  
Time Lapse Imaging ◽  
The Impact

Amlodipine, a unique long-lasting calcium channel antagonist and antihypertensive drug, has weak fluorescence in aqueous solutions. In the current paper, we show that direct visualization of amlodipine in live cells is possible due to the enhanced emission in cellular environment. We examined the impact of pH, polarity and viscosity of the environment as well as protein binding on the spectral properties of amlodipine in vitro, and used quantum chemical calculations for assessing the mechanism of fluorescence quenching in aqueous solutions. The confocal fluorescence microscopy shows that the drug readily penetrates the plasma membrane and accumulates in the intracellular vesicles. Visible emission and photostability of amlodipine allow confocal time-lapse imaging and the drug uptake monitoring.

scholarly journals A genetically encoded cyclobutene probe for labelling of live cells

2017 ◽  
Vol 53 (76) ◽  
pp. 10604-10607 ◽  
Author(s):  
K. Liu ◽  
B. Enns ◽  
B. Evans ◽  
N. Wang ◽  
X. Shang ◽  
...  
Keyword(s):  
Live Cells ◽  
Efficient Synthesis ◽  
Protein Labelling

Efficient synthesis of and protein labelling with a genetically encoded cyclobutene probe.

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