A Ni-NTA-based red fluorescence probe for protein labelling in live cells

2017 ◽  
Vol 5 (6) ◽  
pp. 1166-1173 ◽  
Author(s):  
Ailun Chao ◽  
Nan Jiang ◽  
Ya Yang ◽  
Hongyan Li ◽  
Hongzhe Sun
Keyword(s):  
Protein Purification ◽  
Fluorescence Probe ◽  
Live Cells ◽  
Great Success ◽  
Protein Labelling ◽  
Red Fluorescence

The great success of a His6-Ni-nitrilotriaceate (Ni-NTA) system in protein purification has inspired scientists to develop novel Ni-NTA based fluoresent probes for imaging of proteins in live cells.

Reversible Off–On Fluorescence Probe for Hypoxia and Imaging of Hypoxia–Normoxia Cycles in Live Cells

2012 ◽  
Vol 134 (48) ◽  
pp. 19588-19591 ◽  
Author(s):  
Shodai Takahashi ◽  
Wen Piao ◽  
Yuriko Matsumura ◽  
Toru Komatsu ◽  
Tasuku Ueno ◽  
...  
Keyword(s):  
Fluorescence Probe ◽  
Live Cells

scholarly journals A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Qixin Chen ◽  
Hongbao Fang ◽  
Xintian Shao ◽  
Zhiqi Tian ◽  
Shanshan Geng ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Fluorescent Probes ◽  
Living Cells ◽  
Molecular Probe ◽  
Live Cells ◽  
Blue Fluorescence ◽  
Red Fluorescence ◽  
Dynamic Tracking ◽  
A Cell

AbstractMitochondria–lysosome interactions are essential for maintaining intracellular homeostasis. Although various fluorescent probes have been developed to visualize such interactions, they remain unable to label mitochondria and lysosomes simultaneously and dynamically track their interaction. Here, we introduce a cell-permeable, biocompatible, viscosity-responsive, small organic molecular probe, Coupa, to monitor the interaction of mitochondria and lysosomes in living cells. Through a functional fluorescence conversion, Coupa can simultaneously label mitochondria with blue fluorescence and lysosomes with red fluorescence, and the correlation between the red–blue fluorescence intensity indicates the progress of mitochondria–lysosome interplay during mitophagy. Moreover, because its fluorescence is sensitive to viscosity, Coupa allowed us to precisely localize sites of mitochondria–lysosome contact and reveal increases in local viscosity on mitochondria associated with mitochondria–lysosome contact. Thus, our probe represents an attractive tool for the localization and dynamic tracking of functional mitochondria–lysosome interactions in living cells.

Imaging Different Interactions of Mercury and Silver with Live Cells by a Designed Fluorescence Probe Rhodamine B Selenolactone

2010 ◽  
Vol 49 (3) ◽  
pp. 1206-1210 ◽  
Author(s):  
Wen Shi ◽  
Shuna Sun ◽  
Xiaohua Li ◽  
Huimin Ma
Keyword(s):  
Rhodamine B ◽  
Fluorescence Probe ◽  
Live Cells

A dual-emission and large Stokes shift fluorescence probe for real-time discrimination of ROS/RNS and imaging in live cells

2013 ◽  
Vol 49 (18) ◽  
pp. 1862 ◽  
Author(s):  
Ting Guo ◽  
Lei Cui ◽  
Jiaoning Shen ◽  
Rui Wang ◽  
Weiping Zhu ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescence Probe ◽  
Stokes Shift ◽  
Live Cells ◽  
Dual Emission ◽  
Large Stokes Shift ◽  
Time Discrimination

Open-Chain Crown-Ether-Derived Two-Photon Fluorescence Probe for Real-Time Dynamic Biopsy of Mercury Ions

2017 ◽  
Vol 70 (6) ◽  
pp. 705
Author(s):  
Chibao Huang ◽  
Daohai Zhang ◽  
Junle Qu ◽  
Xiaonan Liu ◽  
Guanglian Zhao ◽  
...  
Keyword(s):  
Water Solubility ◽  
Fluorescence Probe ◽  
Stokes Shift ◽  
Cell Permeability ◽  
Photon Absorption ◽  
Live Cells ◽  
Open Chain ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A novel two-photon fluorescence probe for Hg2+ derived from bis(styryl)terephthalonitrile, as a two-photon fluorophore, and bis[2-(2-hydroxyethyl sulfanyl) ethyl]amino group (ionophore), as a novel Hg2+ ligand, was developed. The probe possesses small molecule size, large two-photon absorption cross-section (1067 GM) in H2O, non-cytotoxic effect, long wavelength emission at 588 nm, large Stokes shift (121 nm), excellent photostability, high water solubility, good cell permeability, and pH insensitivity in the biologically relevant range. The probe can selectively detect Hg2+ ions in live cells and living tissues without interference from other metal ions and the membrane-bound probes, and its quenching constant is 8.73 × 105 M–1.

Sign in / Sign up

Export Citation Format

Share Document