Co-self-assembled nanoaggregates of BODIPY amphiphiles for dual colour imaging of live cells

2015 ◽  
Vol 51 (62) ◽  
pp. 12447-12450 ◽  
Author(s):  
Gang Fan ◽  
Yao-Xin Lin ◽  
Le Yang ◽  
Fu-Ping Gao ◽  
Ying-Xi Zhao ◽  
...  
Keyword(s):  
Live Cells ◽  
Boron Dipyrromethene ◽  
Ratiometric Imaging ◽  
Self Assembled ◽  
Dual Colour

Co-self-assembled vesicular nanoparticles of two structurally comparable amphiphilic boron-dipyrromethene (BODIPY) dyes with dequenchable dual colour fluorescence were prepared for ratiometric imaging of live cells.

A Synthetic Fluorescent Mitochondria‐Targeted Sensor for Ratiometric Imaging of Calcium in Live Cells

2019 ◽  
Vol 131 (29) ◽  
pp. 10022-10027
Author(s):  
Diana Pendin ◽  
Rosa Norante ◽  
Andrea De Nadai ◽  
Gaia Gherardi ◽  
Nicola Vajente ◽  
...  
Keyword(s):  
Live Cells ◽  
Ratiometric Imaging

scholarly journals Fast, Accurate Polarization and Polarity Imaging with Polarized Structured Illumination

2019 ◽  
Author(s):  
Karl Zhanghao ◽  
Wenhui Liu ◽  
Meiqi Li ◽  
Xingye Chen ◽  
Chunyan Shan ◽  
...  
Keyword(s):  
Live Cells ◽  
Polarization Modulation ◽  
Optical Transfer Function ◽  
Wide Field ◽  
Structured Illumination ◽  
Optical Sectioning ◽  
Polarization Imaging ◽  
Order Of Magnitude ◽  
Ratiometric Imaging ◽  
Optical Transfer

AbstractThe orientation and wobbling behavior of the fluorescent dipoles are of great significance in revealing the structure and state of cells. Due to the poor optical sectioning capability of wide-field microscopy, the polarization modulation signals are susceptible to the neighboring fluorophores. The missing cone of wide field optical transfer function induces vast out-of-focus background, resulting in biased polarization orientation and decrease polarization factor. Here, we apply polarized structured illumination to achieve polarization modulation imaging with optical sectioning, and simultaneously measure the lipid polarity with two-color ratiometric imaging. Our results demonstrate a significant increase in measurement accuracy of not only the dipole orientations but also the wobbling behavior of the ensemble dipole. Compared to the conventional confocal polarization imaging, our method obtains an order-of-magnitude faster imaging speed, capturing the fast dynamics of subcellular structures in live cells.

A two-photon fluorescent probe for ratiometric visualization of hypochlorous acid in live cells and animals based on a selenide oxidation/elimination tandem reaction

2018 ◽  
Vol 54 (84) ◽  
pp. 11965-11968 ◽  
Author(s):  
Xilei Xie ◽  
Tianhong Wu ◽  
Xu Wang ◽  
Yong Li ◽  
Kaiye Wang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Live Cells ◽  
Tandem Reaction ◽  
Two Photon ◽  
Ratiometric Imaging ◽  
Sensing Performance

A fluorescent probe with superior sensing performance enables two-photon ratiometric imaging of biogenic hypochlorous acid in live cells and animals.

scholarly journals An enzyme-free molecular catalytic device: dynamically self-assembled DNA dendrimers for in situ imaging of microRNAs in live cells

2019 ◽  
Vol 10 (6) ◽  
pp. 1651-1658 ◽  
Author(s):  
Shuzhen Yue ◽  
Xinyue Song ◽  
Weiling Song ◽  
Sai Bi
Keyword(s):  
Self Assembly ◽  
Signal Amplification ◽  
High Sensitivity ◽  
Live Cells ◽  
Sensitivity And Selectivity ◽  
Catalytic Hairpin Assembly ◽  
Self Assembled ◽  
In Situ Imaging

Branched catalytic hairpin assembly is proposed for dynamic self-assembly of DNA dendrimers for signal amplification and in situ imaging of microRNAs in live cells with high sensitivity and selectivity.

scholarly journals Engineering of a zero cross-talk fluorescent polymer nanoprobe for self-referenced ratiometric imaging of lysosomal hypochlorous acid in living cells

2020 ◽  
Vol 4 (3) ◽  
pp. 862-868 ◽  
Author(s):  
Hongqing Wei ◽  
Rongjin Zeng ◽  
Shenglan Wang ◽  
Chong-Hua Zhang ◽  
Shu Chen ◽  
...  
Keyword(s):  
Cross Talk ◽  
Hypochlorous Acid ◽  
Living Cells ◽  
Live Cells ◽  
Ratiometric Imaging ◽  
Fluorescent Polymer

A zero cross-talk fluorescent polymer nanoprobe with a self-referenced ratiometric effect was rationally designed and synthesized for lysosomal hypochlorous acid (HClO) imaging in live cells.

A Synthetic Fluorescent Mitochondria‐Targeted Sensor for Ratiometric Imaging of Calcium in Live Cells

2019 ◽  
Vol 58 (29) ◽  
pp. 9917-9922 ◽  
Author(s):  
Diana Pendin ◽  
Rosa Norante ◽  
Andrea De Nadai ◽  
Gaia Gherardi ◽  
Nicola Vajente ◽  
...  
Keyword(s):  
Live Cells ◽  
Ratiometric Imaging

A nucleus targetable fluorescent probe for ratiometric imaging of endogenous NO in living cells and zebrafishes

The Analyst ◽  
2021 ◽  
Author(s):  
Liang Zhao ◽  
Zhenlong Huang ◽  
Daqing Ma ◽  
Yu Yan ◽  
Xinfu Zhang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Live Cells ◽  
Real Time Monitoring ◽  
Immune Systems ◽  
Ratiometric Imaging ◽  
Messenger Molecule

Nitric oxide (NO) is an important cellular messenger molecule in the cardiovascular, nervous and immune systems. Real-time monitoring of NO activity in specific organelles of live cells is important to...

A cysteamine-selective two-photon fluorescent probe for ratiometric bioimaging

2015 ◽  
Vol 51 (12) ◽  
pp. 2407-2410 ◽  
Author(s):  
Avik R. Sarkar ◽  
Cheol Ho Heo ◽  
Eunjin Kim ◽  
Hyo Won Lee ◽  
Hardev Singh ◽  
...  
Keyword(s):  
Rat Brain ◽  
Fluorescent Probe ◽  
Live Cells ◽  
Two Photon ◽  
Ratiometric Imaging

A two-photon fluorescent probe for ratiometric imaging of cysteamine in live cells and rat brain hippocampal tissues was reported.

scholarly journals Correction: A versatile two-photon fluorescent probe for ratiometric imaging E. coli β-galactosidase in live cells and in vivo

2016 ◽  
Vol 52 (60) ◽  
pp. 9470-9470 ◽  
Author(s):  
Xue-Xiang Zhang ◽  
Hao Wu ◽  
Peng Li ◽  
Zong-Jin Qu ◽  
Ming-Qian Tan ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Live Cells ◽  
E Coli ◽  
Two Photon ◽  
Ratiometric Imaging

Correction for ‘A versatile two-photon fluorescent probe for ratiometric imaging E. coli β-galactosidase in live cells and in vivo’ by Xue-Xiang Zhang et al., Chem. Commun., 2016, 52, 8283–8286.

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