scholarly journals Engineering of a zero cross-talk fluorescent polymer nanoprobe for self-referenced ratiometric imaging of lysosomal hypochlorous acid in living cells

2020 ◽  
Vol 4 (3) ◽  
pp. 862-868 ◽  
Author(s):  
Hongqing Wei ◽  
Rongjin Zeng ◽  
Shenglan Wang ◽  
Chong-Hua Zhang ◽  
Shu Chen ◽  
...  
Keyword(s):  
Cross Talk ◽  
Hypochlorous Acid ◽  
Living Cells ◽  
Live Cells ◽  
Ratiometric Imaging ◽  
Fluorescent Polymer

A zero cross-talk fluorescent polymer nanoprobe with a self-referenced ratiometric effect was rationally designed and synthesized for lysosomal hypochlorous acid (HClO) imaging in live cells.

Ratiometric imaging of lysosomal hypochlorous acid enabled by FRET-based polymer dots

2017 ◽  
Vol 8 (37) ◽  
pp. 5795-5802 ◽  
Author(s):  
Hong Wang ◽  
Peisheng Zhang ◽  
Yongxiang Hong ◽  
Bin Zhao ◽  
Pinggui Yi ◽  
...  
Keyword(s):  
Membrane Permeability ◽  
Hypochlorous Acid ◽  
Living Cells ◽  
Polymer Dots ◽  
Ratiometric Imaging ◽  
Fluorescent Polymer

FRET-based fluorescent polymer dots (FPD) with good membrane permeability have been developed for ratiometric imaging of lysosomal HClO in living cells.

A two-photon fluorescent probe for ratiometric visualization of hypochlorous acid in live cells and animals based on a selenide oxidation/elimination tandem reaction

2018 ◽  
Vol 54 (84) ◽  
pp. 11965-11968 ◽  
Author(s):  
Xilei Xie ◽  
Tianhong Wu ◽  
Xu Wang ◽  
Yong Li ◽  
Kaiye Wang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Live Cells ◽  
Tandem Reaction ◽  
Two Photon ◽  
Ratiometric Imaging ◽  
Sensing Performance

A fluorescent probe with superior sensing performance enables two-photon ratiometric imaging of biogenic hypochlorous acid in live cells and animals.

A nucleus targetable fluorescent probe for ratiometric imaging of endogenous NO in living cells and zebrafishes

The Analyst ◽  
2021 ◽  
Author(s):  
Liang Zhao ◽  
Zhenlong Huang ◽  
Daqing Ma ◽  
Yu Yan ◽  
Xinfu Zhang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Live Cells ◽  
Real Time Monitoring ◽  
Immune Systems ◽  
Ratiometric Imaging ◽  
Messenger Molecule

Nitric oxide (NO) is an important cellular messenger molecule in the cardiovascular, nervous and immune systems. Real-time monitoring of NO activity in specific organelles of live cells is important to...

A fluorescent probe for ratiometric imaging of exogenous and intracellular formed hypochlorous acid in lysosomes

2017 ◽  
Vol 41 (13) ◽  
pp. 5259-5262 ◽  
Author(s):  
Mingguang Ren ◽  
Jing Nie ◽  
Beibei Deng ◽  
Kai Zhou ◽  
Jian-Yong Wang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Small Molecule ◽  
Hypochlorous Acid ◽  
Living Cells ◽  
Ratiometric Imaging

We have developed a new small-molecule based lysosome-targeted ratiometric fluorescent HOCl probe (FL-HA). Fluorescence imaging shows that FL-HA is suitable for ratiometric visualization of HOCl within lysosomes in living cells.

A two-photon fluorescent probe for ratiometric imaging of endogenous hypochlorous acid in live cells and tissues

2017 ◽  
Vol 53 (78) ◽  
pp. 10800-10803 ◽  
Author(s):  
Yong Woong Jun ◽  
Sourav Sarkar ◽  
Subhankar Singha ◽  
Ye Jin Reo ◽  
Hye Rim Kim ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Reactive Oxygen ◽  
Live Cells ◽  
Oxygen Species ◽  
Two Photon ◽  
Ratiometric Imaging ◽  
In Cells

A fluorescent probe enables two-photon ratiometric imaging of endogenous hypochlorous acid, a reactive oxygen species, in cells and tissues.

A self-referenced nanodosimeter for reaction based ratiometric imaging of hypochlorous acid in living cells

2013 ◽  
Vol 4 (1) ◽  
pp. 460-467 ◽  
Author(s):  
Xuanjun Wu ◽  
Zhu Li ◽  
Liu Yang ◽  
Jiahuai Han ◽  
Shoufa Han
Keyword(s):  
Hypochlorous Acid ◽  
Living Cells ◽  
Ratiometric Imaging

4-dimensional, high-resolution imaging of living cells

1992 ◽  
Vol 50 (1) ◽  
pp. 416-417
Author(s):  
Shinya Inoué
Keyword(s):  
Light Microscope ◽  
Living Cells ◽  
Live Cells ◽  
Video Tape ◽  
Active Living ◽  
High Resolution Imaging ◽  
3 Dimensional ◽  
Dynamic Architecture ◽  
Resolution Imaging ◽  
Disk Memory

This paper reports progress of our effort to rapidly capture, and display in time-lapsed mode, the 3-dimensional dynamic architecture of active living cells and developing embryos at the highest resolution of the light microscope. Our approach entails: (A) real-time video tape recording of through-focal, ultrathin optical sections of live cells at the highest resolution of the light microscope; (B) repeat of A at time-lapsed intervals; (C) once each time-lapsed interval, an image at home focus is recorded onto Optical Disk Memory Recorder (OMDR); (D) periods of interest are selected using the OMDR and video tape records; (E) selected stacks of optical sections are converted into plane projections representing different view angles (±4 degrees for stereo view, additional angles when revolving stereos are desired); (F) analysis using A - D.

scholarly journals 3D super-resolved imaging in live cells using sub-diffractive plasmonic localization of hybrid nanopillar arrays

Nanophotonics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2847-2859
Author(s):  
Soojung Kim ◽  
Hyerin Song ◽  
Heesang Ahn ◽  
Seung Won Jun ◽  
Seungchul Kim ◽  
...  
Keyword(s):  
Signal To Noise Ratio ◽  
Imaging Techniques ◽  
Optical Loss ◽  
Super Resolution ◽  
Living Cells ◽  
Live Cells ◽  
Complex Biological System ◽  
Observation Volume ◽  
Resolution Imaging ◽  
Nanopillar Arrays

AbstractAnalysing dynamics of a single biomolecule using high-resolution imaging techniques has been had significant attentions to understand complex biological system. Among the many approaches, vertical nanopillar arrays in contact with the inside of cells have been reported as a one of useful imaging applications since an observation volume can be confined down to few-tens nanometre theoretically. However, the nanopillars experimentally are not able to obtain super-resolution imaging because their evanescent waves generate a high optical loss and a low signal-to-noise ratio. Also, conventional nanopillars have a limitation to yield 3D information because they do not concern field localization in z-axis. Here, we developed novel hybrid nanopillar arrays (HNPs) that consist of SiO2 nanopillars terminated with gold nanodisks, allowing extreme light localization. The electromagnetic field profiles of HNPs are obtained through simulations and imaging resolution of cell membrane and biomolecules in living cells are tested using one-photon and 3D multiphoton fluorescence microscopy, respectively. Consequently, HNPs present approximately 25 times enhanced intensity compared to controls and obtained an axial and lateral resolution of 110 and 210 nm of the intensities of fluorophores conjugated with biomolecules transported in living cells. These structures can be a great platform to analyse complex intracellular environment.

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