A new strategy for site-specific alkylation of DNA using oligonucleotides containing an abasic site and alkylating probes

2015 ◽  
Vol 51 (80) ◽  
pp. 14885-14888 ◽  
Author(s):  
Norihiro Sato ◽  
Genichiro Tsuji ◽  
Yoshihiro Sasaki ◽  
Akira Usami ◽  
Takuma Moki ◽  
...  
Keyword(s):  
Abasic Site ◽  
Site Specific ◽  
New Strategy ◽  
Selective Alkylation

In this communication, we report a new strategy for the selective alkylation of DNA using an oligonucleotide containing an abasic site and alkylating probes.

scholarly journals pH-dependent gating mechanism of theHelicobacter pyloriurea channel revealed by cryo-EM

2019 ◽  
Vol 5 (3) ◽  
pp. eaav8423 ◽  
Author(s):  
Yanxiang Cui ◽  
Kang Zhou ◽  
David Strugatsky ◽  
Yi Wen ◽  
George Sachs ◽  
...  
Keyword(s):  
Drug Target ◽  
Carboxyl Terminus ◽  
Site Specific ◽  
Gating Mechanism ◽  
New Strategy ◽  
Periplasmic Domain ◽  
Urea Channel ◽  
Ph Dependent ◽  
H Pylori ◽  
Key Residues

The urea channel ofHelicobacterpylori(HpUreI) is an ideal drug target for preventing gastric cancer but incomplete understanding of its gating mechanism has hampered development of inhibitors for the eradication ofH. pylori. Here, we present the cryo-EM structures ofHpUreI in closed and open conformations, both at a resolution of 2.7 Å. Our hexameric structures of this small membrane protein (~21 kDa/protomer) resolve its periplasmic loops and carboxyl terminus that close and open the channel, and define a gating mechanism that is pH dependent and requires cooperativity between protomers in the hexamer. Gating is further associated with well-resolved changes in the channel-lining residues that modify the shape and length of the urea pore. Site-specific mutations in the periplasmic domain and urea pore identified key residues important for channel function. Drugs blocking the urea pore based on our structures should lead to a new strategy forH. pylorieradication.

A New Strategy for the Site-Specific Modification of Proteinsin Vivo†

Biochemistry ◽  
2003 ◽  
Vol 42 (22) ◽  
pp. 6735-6746 ◽  
Author(s):  
Zhiwen Zhang ◽  
Brian A. C. Smith ◽  
Lei Wang ◽  
Ansgar Brock ◽  
Charles Cho ◽  
...  
Keyword(s):  
Site Specific ◽  
Specific Modification ◽  
New Strategy

scholarly journals New Strategy for Reversible Modulation of Protein Activity through Site-Specific Conjugation of Small Molecule and Polymer

2014 ◽  
Vol 25 (7) ◽  
pp. 1252-1260 ◽  
Author(s):  
Lei Wang ◽  
Lin Yuan ◽  
Hongwei Wang ◽  
Xiaoli Liu ◽  
Xinming Li ◽  
...  
Keyword(s):  
Small Molecule ◽  
Protein Activity ◽  
Site Specific ◽  
New Strategy

Hyaluronic acid conjugated polydopamine functionalized mesoporous silica nanoparticles for synergistic targeted chemo-photothermal therapy

Nanoscale ◽  
2019 ◽  
Vol 11 (22) ◽  
pp. 11012-11024 ◽  
Author(s):  
Chao Chen ◽  
Wen Tang ◽  
Dawei Jiang ◽  
Guoliang Yang ◽  
Xiaoli Wang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Hyaluronic Acid ◽  
Photothermal Therapy ◽  
Drug Delivery Systems ◽  
Mesoporous Silica Nanoparticles ◽  
Site Specific ◽  
Functionalized Mesoporous Silica ◽  
Nano Drug Delivery ◽  
New Strategy ◽  
Surface Fabrication

This work reports a new strategy in surface fabrication of nano-drug delivery systems to achieve site-specific synergetic chemo-photothermal therapy.

scholarly journals Design of targeted dosage form of ofloxacin+

2006 ◽  
Vol 71 (12) ◽  
pp. 1269-1273 ◽  
Author(s):  
Yar Shahar ◽  
Ahamed Siddiqui ◽  
Ashraf Ali ◽  
E. Manogran
Keyword(s):  
Drug Design ◽  
Specific Activity ◽  
In Vitro Release ◽  
Polymer Backbone ◽  
Specific Chemical ◽  
Site Specific ◽  
Development Of Resistance ◽  
Quinolone Antibiotics ◽  
New Strategy

The targeted pro-drug is a classical pro-drug design often representing a non-specific chemical approach to mask undesirable drug properties, such as limited bioavailability, lack of site specificity, and chemical instability. On the other hand, targeted pro-drug design represents a new strategy for directed and efficient drug delivery. Quinolone antibiotics exert their pharmacological effect by inhibiting the cell wall synthesis of the pathogen. However, development of resistance exists, which instigates for a new higher congener to remain in clinical practice. To overcome this phenomenon and also to produce site-specific activity of the cell walls of the pathogen, ofloxacin is conjugated with a hydroxypropyl methacrylamide polymer backbone moiety. The results of in vitro release studies indicate the possibilities for the development of a new drug for site-specific therapy with an improved t 1/2 of the drug. This novel pro-drugmay have opened a new vista in antibiotic chemotherapy. .

A New Strategy for Site-Specific Protein Modification:  Analysis of a Tat Peptide−TAR RNA Interaction

2001 ◽  
Vol 12 (2) ◽  
pp. 135-138 ◽  
Author(s):  
Natarajan Tamilarasu ◽  
Jing Zhang ◽  
Seongwoo Hwang ◽  
Tariq M. Rana
Keyword(s):  
Protein Modification ◽  
Specific Protein ◽  
Tat Peptide ◽  
Site Specific ◽  
New Strategy ◽  
Tar Rna ◽  
Rna Interaction

Site-Specific Cross-Linking of Nucleic Acids Using the Abasic Site

1999 ◽  
Vol 1 (2) ◽  
pp. 311-314 ◽  
Author(s):  
Muthiah Manoharan ◽  
Laura K. Andrade ◽  
P. Dan Cook
Keyword(s):  
Nucleic Acids ◽  
Cross Linking ◽  
Abasic Site ◽  
Site Specific

Site-specific Ag coating on concave Au nanoarrows by controlling the surfactant concentration

2019 ◽  
Vol 4 (4) ◽  
pp. 940-946 ◽  
Author(s):  
Dashen Dong ◽  
Qianqian Shi ◽  
Debabrata Sikdar ◽  
Yunmeng Zhao ◽  
Yiyi Liu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Surfactant Concentration ◽  
Particle Morphology ◽  
Elemental Mapping ◽  
Site Specific ◽  
Growth Solution ◽  
Distinct Site ◽  
New Strategy

Three distinct site-specific Ag-coated Au nanoarrows are achieved by simply controlling the CTAC concentration of growth solution and analyzed with electron microscopy as well as elemental mapping. The CTAC concentration-dependent, facet-specific passivation represents a new strategy to design particle morphology as well as composition.

Abasic Site Recognition in DNA as a New Strategy To Potentiate the Action of Anticancer Alkylating Drugs?

1999 ◽  
Vol 42 (25) ◽  
pp. 5153-5159 ◽  
Author(s):  
Philippe Belmont ◽  
Muriel Jourdan ◽  
Martine Demeunynck ◽  
Jean-François Constant ◽  
Julian Garcia ◽  
...  
Keyword(s):  
Abasic Site ◽  
Alkylating Drugs ◽  
New Strategy ◽  
Site Recognition
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