The determination of DNA methyltransferase activity by quenching of tris(2,2′-bipyridine)ruthenium electrogenerated chemiluminescence with ferrocene

2015 ◽  
Vol 51 (46) ◽  
pp. 9487-9490 ◽  
Author(s):  
Xiaoe Luo ◽  
Yan Li ◽  
Jianbin Zheng ◽  
Honglan Qi ◽  
Zhenxing Liang ◽  
...  
Keyword(s):  
Dna Methyltransferase ◽  
Electrogenerated Chemiluminescence ◽  
Methyltransferase Activity

An electrogenerated chemiluminescence biosensing method for the determination of DNA methyltransferase activity is developed by the quenching of tris(2,2′-bipyridine)ruthenium ECL by ferrocene.

A sensitive cyclic signal amplification fluorescence strategy for determination of methyltransferase activity based on graphene oxide and RNase H

2019 ◽  
Vol 7 (29) ◽  
pp. 4520-4527 ◽  
Author(s):  
Han Zhao ◽  
Changbei Ma ◽  
Ying Yan ◽  
Mingjian Chen
Keyword(s):  
Graphene Oxide ◽  
Signal Amplification ◽  
Dna Methyltransferase ◽  
Rnase H ◽  
Activity Detection ◽  
Methyltransferase Activity ◽  
Fluorometric Method

A sensitive fluorometric method for DNA methyltransferase activity detection based on graphene oxide and RNase H-assisted signal amplification.

scholarly journals Reduced Genomic Cytosine Methylation and Defective Cellular Differentiation in Embryonic Stem Cells Lacking CpG Binding Protein

2005 ◽  
Vol 25 (12) ◽  
pp. 4881-4891 ◽  
Author(s):  
Diana L. Carlone ◽  
Jeong-Heon Lee ◽  
Suzanne R. L. Young ◽  
Erika Dobrota ◽  
Jill Sergesketter Butler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Dna Methyltransferase ◽  
Cellular Differentiation ◽  
Cytosine Methylation ◽  
Epigenetic Modification ◽  
Binding Protein ◽  
Embryonic Stem ◽  
Growth Media ◽  
Methyltransferase Activity

ABSTRACT Cytosine methylation at CpG dinucleotides is a critical epigenetic modification of mammalian genomes. CpG binding protein (CGBP) exhibits a unique DNA-binding specificity for unmethylated CpG motifs and is essential for early murine development. Embryonic stem cell lines deficient for CGBP were generated to further examine CGBP function. CGBP − / − cells are viable but show an increased rate of apoptosis and are unable to achieve in vitro differentiation following removal of leukemia inhibitory factor from the growth media. Instead, CGBP − / − embryonic stem cells remain undifferentiated as revealed by persistent expression of the pluripotent markers Oct4 and alkaline phosphatase. CGBP − / − cells exhibit a 60 to 80% decrease in global cytosine methylation, including hypo-methylation of repetitive elements, single-copy genes, and imprinted genes. Total DNA methyltransferase activity is reduced by 30 to 60% in CGBP − / − cells, and expression of the maintenance DNA methyltransferase 1 protein is similarly reduced. However, de novo DNA methyltransferase activity is normal. Nearly all aspects of the pleiotropic CGBP − / − phenotype are rescued by introduction of a CGBP expression vector. Hence, CGBP is essential for normal epigenetic modification of the genome by cytosine methylation and for cellular differentiation, consistent with the requirement for CGBP during early mammalian development.

Determination of amitriptyline using electrogenerated chemiluminescence

1996 ◽  
Vol 33 (4) ◽  
pp. 139 ◽  
Author(s):  
Sarah J. L. Dolman ◽  
Gillian M. Greenway
Keyword(s):  
Electrogenerated Chemiluminescence
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