Assembly of bioactive multilayered nanocoatings on pancreatic islet cells: incorporation of α1-antitrypsin into the coatings

2015 ◽  
Vol 51 (53) ◽  
pp. 10652-10655 ◽  
Author(s):  
Zheng-Liang Zhi ◽  
Jashandeep Singh ◽  
Amazon L. F. Austin ◽  
David C. D. Hope ◽  
Aileen J. King ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Pancreatic Islet ◽  
Inflammatory Reaction ◽  
Islet Cells ◽  
Therapeutic Proteins ◽  
Pancreatic Islet Cells ◽  
Glycol Chitosan ◽  
Multilayer Deposition

A novel multilayer deposition approach to the delivery of therapeutic proteins onto the surface of pancreatic islets, using a heparin polyaldehyde and glycol chitosan alternating layering scheme, has been developed for addressing the blood-mediated inflammatory reaction against islet cells.

scholarly journals The coupling of metabolic to secretory events in pancreatic islets. The cytosolic redox state

1984 ◽  
Vol 220 (2) ◽  
pp. 433-440 ◽  
Author(s):  
A Sener ◽  
F Malaisse-Lagae ◽  
S P Dufrane ◽  
W J Malaisse
Keyword(s):  
Pancreatic Islets ◽  
Malate Dehydrogenase ◽  
Insulin Release ◽  
Pancreatic Islet ◽  
Flow Rate ◽  
Isocitrate Dehydrogenase ◽  
Malic Enzyme ◽  
Redox State ◽  
Islet Cells ◽  
Pancreatic Islet Cells

NADP-linked isocitrate dehydrogenase and malic enzyme [malate dehydrogenase (decarboxylating) (NADP)] activities were characterized in the cytosol of pancreatic islet cells. D-Glucose and L-leucine augmented the cytosolic NADPH/NADP+ ratio, as judged from the isocitrate/2-oxoglutarate and malate/pyruvate islet contents. The flow rate through the malic enzyme was judged from the output of labelled pyruvate by islets exposed to either L-[U-14C]glutamine or L-[U-14C]leucine. The cytosolic generation of NADPH, e.g. at the level of the malic enzyme, may play a role in the coupling of metabolic to secretory events in the process of nutrient-stimulated insulin release.

scholarly journals Expression of Insulinoma-Associated 2 (INSM2) in Pancreatic Islet Cells Is Regulated by the Transcription Factors Ngn3 and NeuroD1

Endocrinology ◽  
2011 ◽  
Vol 152 (5) ◽  
pp. 1961-1969 ◽  
Author(s):  
Tao Cai ◽  
Xiang Chen ◽  
Rennian Wang ◽  
Huan Xu ◽  
Yuhui You ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Pancreatic Islets ◽  
Pancreatic Islet ◽  
Chromatin Immunoprecipitation ◽  
Islet Cells ◽  
Mouse Embryos ◽  
Pancreatic Islet Cells ◽  
Neurogenin 3 ◽  
Fetal Pancreas ◽  
E Boxes

The insulinoma-associated 2 (Insm2) gene is a member of the Snail/Gfi1/Insm1 transcriptional repressor superfamily. However, little is known about how the expression of human INSM2 or mouse Insm2 in neuroendocrine tissues is regulated. Here we report the expression of INSM2/Insm2 in human fetal pancreas and mouse embryos, as well as adult pancreatic islets, and its regulation by two major islet transcription factors. Mutagenesis and chromatin immunoprecipitation analysis demonstrated that the proximal E-boxes of the mouse Insm2 promoter are direct targets of neurogenin 3 and neurogenic differentiation 1 (NeuroD1). Furthermore, we found that endogenous Insm2 expression was activated in Ngn3/NeuroD1-transduced pancreatic epithelial duct cells. Our results suggest that Insm2 plays an important role in the differentiation cascade of Ngn3/NeuroD1 signaling in pancreatic islets.

scholarly journals The microsomal glucose-6-phosphatase enzyme of pancreatic islets

1988 ◽  
Vol 255 (2) ◽  
pp. 471-476 ◽  
Author(s):  
I D Waddell ◽  
A Burchell
Keyword(s):  
Pancreatic Islets ◽  
Phosphatase Activity ◽  
Pancreatic Islet ◽  
Islet Cell ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Kinetic Characteristics ◽  
Immunological Properties ◽  
Cell Glucose ◽  
Phosphatase Enzyme

Microsomal fractions isolated from pancreatic islet cells were shown to contain high specific glucose-6-phosphatase activity. The islet-cell glucose-6-phosphatase enzyme has the same Mr (36,500), similar immunological properties and kinetic characteristics to the hepatic microsomal glucose-6-phosphatase enzyme.

Monolayer cultures of adult pancreatic islet cells on osmotically disrupted fibroblasts

Diabetes ◽  
1980 ◽  
Vol 29 (6) ◽  
pp. 497-500 ◽  
Author(s):  
P. Meda ◽  
E. L. Hooghe-Peters ◽  
L. Orci
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Monolayer Cultures

Use of liposomes to introduce substances into pancreatic islet cells

Diabetes ◽  
1988 ◽  
Vol 37 (8) ◽  
pp. 1123-1128
Author(s):  
N. Welsh ◽  
A. Hallberg ◽  
S. Sandler ◽  
C. Hellerstrom
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells

Superfusion of dissociated pancreatic islet cells attached to Cytodex beads

Diabetes ◽  
1982 ◽  
Vol 31 (3) ◽  
pp. 189-193 ◽  
Author(s):  
Y. Spiess ◽  
M. A. Smith ◽  
W. Vale
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells

Efficient gene transfer to dispersed human pancreatic islet cells in vitro using adenovirus-polylysine/DNA complexes or polycationic liposomes

Diabetes ◽  
1996 ◽  
Vol 45 (9) ◽  
pp. 1197-1203 ◽  
Author(s):  
J. Saldeen ◽  
D. T. Curiel ◽  
D. L. Eizirik ◽  
A. Andersson ◽  
E. Strandell ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Dna Complexes ◽  
Human Pancreatic Islet ◽  
Efficient Gene
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