An ultrasensitive ELISA method for the detection of procalcitonin based on magnetic beads and enzyme-antibody labeled gold nanoparticles

2016 ◽  
Vol 8 (7) ◽  
pp. 1577-1585 ◽  
Author(s):  
Tao Liao ◽  
Fang Yuan ◽  
Haoyang Yu ◽  
Zigang Li
Keyword(s):  
Gold Nanoparticles ◽  
Severe Sepsis ◽  
Mortality Rate ◽  
Bacterial Infection ◽  
Magnetic Beads ◽  
Specific Marker ◽  
Elisa Method ◽  
Significant Mortality

Sepsis is a potentially fatal systemic body infection with a significant mortality rate worldwide. Procalcitonin (PCT) is a specific marker for severe sepsis caused by bacterial infection. Herein, an ultrasensitive ELISA method based on magnetic beads and enzyme-antibody labeled gold nanoparticles was reported for the detection of PCT.

Delivery of miR-320a-3p by gold nanoparticles combined with photothermal therapy for directly targeting Sp1 in lung cancer

2021 ◽  
Author(s):  
Jiefei Peng ◽  
Ranran Wang ◽  
Wanru Sun ◽  
Minhua Huang ◽  
Rong Wang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Overall Survival ◽  
Gold Nanoparticles ◽  
Mortality Rate ◽  
Photothermal Therapy ◽  
Therapeutic Targets ◽  
Novel Therapeutic

Lung cancer is the second most common tumor and has the highest mortality rate. Both novel therapeutic targets and approaches are needed to improve the overall survival of patients with...

A colorimetric aptasensor for the antibiotics oxytetracycline and kanamycin based on the use of magnetic beads and gold nanoparticles

2018 ◽  
Vol 185 (12) ◽  
Author(s):  
Yuanyuan Xu ◽  
Chenhe Lu ◽  
Yangyang Sun ◽  
Yingge Shao ◽  
Ying Cai ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Magnetic Beads ◽  
Colorimetric Aptasensor

scholarly journals The mortality of Oryzaephilus surinamensis Linnaeus, 1758 (Coleoptera: Silvanidae) induced by powdered plants

2015 ◽  
Vol 55 (1) ◽  
pp. 110-116 ◽  
Author(s):  
Małgorzata Kłyś ◽  
Anna Przystupińska
Keyword(s):  
Mortality Rate ◽  
Relative Humidity ◽  
Allium Sativum ◽  
Salvia Officinalis ◽  
Mentha Piperita ◽  
Oryzaephilus Surinamensis ◽  
Artemisia Absinthium ◽  
Pimenta Dioica ◽  
Salvia Officinalis L ◽  
Significant Mortality

Abstract The aim of this study was to investigate whether powdered plants of different species namely: peppermint Mentha piperita (L.) (Lamiaceae), wormwood Artemisia absinthium (L.) (Asteraceae), common sage Salvia officinalis (L.) (Lamiaceae), allspice Pimenta dioica (Linnaeus et Merrill) (Myrtaceae) and common garlic Allium sativum (L.) (Amaryllidaceae), added to semolina using concentrations of 1.23, 3.61, and 5.88%, influence the mortality rate in the saw-toothed grain beetle Oryzaephilus surinamensis Linnaeus, 1758 (Coleoptera: Silvanidae). Experiments were conducted in a laboratory at 28°C and relative humidity 60±5%. At the concentration of 1.23%, allspice seeds caused the highest mortality amongst the saw-toothed grain beetle. When concentrations of 3.61 and 5.88% were used, sage, peppermint and wormwood caused the highest statistically significant mortality of O. surinamensis

Diagnosis of tuberculosis by nanoparticle-based immuno-PCR assay based on mycobacterial MPT64 and CFP-10 detection

Nanomedicine ◽  
2020 ◽  
Vol 15 (26) ◽  
pp. 2609-2624
Author(s):  
Bhawna Dahiya ◽  
Tulika Prasad ◽  
Vishwajeet Singh ◽  
Anish Khan ◽  
Ekta Kamra ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Mycobacterium Tuberculosis ◽  
Diagnostic Accuracy ◽  
Magnetic Beads ◽  
Pcr Assay ◽  
Pulmonary Tb ◽  
Functionalized Gold Nanoparticles ◽  
Bodily Fluids ◽  
Smear Negative ◽  
Improved Technology

Aim: To improve the diagnostic accuracy of immuno-PCR (I-PCR) in tuberculosis (TB) patients by using functionalized gold nanoparticles (AuNPs) coupled with detection antibodies and oligonucleotides, and magnetic beads (MBs) conjugated with capture antibodies in the liquid phase. Materials & methods: MB-coupled AuNP-based I-PCR (MB-AuNP-I-PCR) assay was designed to detect a cocktail of Mycobacterium tuberculosis MPT64 and CFP-10 proteins in bodily fluids of TB patients. Results: The sensitivities of 89.3 (n = 94) and 78.1% (n = 73) were observed in pulmonary TB and extrapulmonary TB patients, respectively, with specificities of 97.9–98.3%. Notably, the sensitivities attained by MB-AuNP-I-PCR in smear-negative pulmonary TB and extrapulmonary TB patients were significantly higher (p < 0.05–0.001) than Magneto-ELISA and GeneXpert assay. Conclusion: The improved technology, as well as enhanced diagnostic accuracy of MB-AuNP-I-PCR, may lead to development of an attractive diagnostic kit.

Ureteroarterial fistula: A review of the literature

Vascular ◽  
2015 ◽  
Vol 24 (2) ◽  
pp. 203-207 ◽  
Author(s):  
Akhil Das ◽  
Patricia Lewandoski ◽  
Dean Laganosky ◽  
John Walton ◽  
Patrick Shenot
Keyword(s):  
Urinary Tract ◽  
Mortality Rate ◽  
Literature Review ◽  
Blood Vessels ◽  
Early Recognition ◽  
Review Of The Literature ◽  
Endovascular Stenting ◽  
Surgical Closure ◽  
Recent Treatment ◽  
Significant Mortality

Ureteroarterial fistulas are rare, erosive defects that occur between the distal segments of the ureter and the adjacent blood vessels in individuals with urologic and vascular comorbidities. Characterized by diffuse, pulsatile bleeding into the urinary tract, this condition carries a significant mortality rate in the absence of early recognition. Recent treatment efforts have focused on use of endovascular stenting techniques as an alternative to open surgical closure of the underlying defects in hemodynamically stable patients. We provide a literature review detailing the characteristics, mechanism, and management of ureteroarterial fistulas.

scholarly journals Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Chi-Fang Peng ◽  
Xiao-Hui Duan ◽  
Qiu-Li Pan ◽  
Li-Qiang Liu ◽  
Feng Xue
Keyword(s):  
Gold Nanoparticles ◽  
Rapid Screening ◽  
Veterinary Drug ◽  
Animal Tissues ◽  
Elisa Method ◽  
Veterinary Drug Residue ◽  
Highly Sensitive ◽  
Chicken Tissue ◽  
Signal Probe ◽  
Sensitive Method

It is challenging to obtain a highly sensitive enzyme-linked sorbent immunoassay (ELISA) method for the rapid screening of veterinary drug residue in animal tissues. Here we present that a simple and sensitive detection for sulfadimethoxine (SDM) residue in animal tissues was achieved by utilizing a bioconjugate of gold nanoparticles and enzyme-labeled antibody as signal probe in enzyme-linked sorbent immunoassay (ELISA). The developed nano-ELISA has increased the sensitivity of a traditional ELISA for SDM by 20-fold. The sensitivity of this ELISA was 5 pg/mL in buffer, and the detection limit (LOD) of 0.2 µg/kg can be obtained after chicken liver was simply extracted by buffer. This simple and sensitive method can be used to improve the sensitivity of ELISA methods for various small molecule contaminants.

Epidemiology of Sepsis in the Valencian Community (Spain), 1995–2004

2008 ◽  
Vol 29 (7) ◽  
pp. 630-634 ◽  
Author(s):  
Juan Carlos Andreu Ballester ◽  
Ferran Ballester ◽  
Antonio González Sánchez ◽  
Amadeo Almela Quilis ◽  
Enrique Colomer Rubio ◽  
...  
Keyword(s):  
Severe Sepsis ◽  
Mortality Rate ◽  
Confidence Interval ◽  
Organ Failure ◽  
Incidence Rates ◽  
Public Hospitals ◽  
Polymicrobial Sepsis ◽  
Gram Negative Bacteria ◽  
Gram Negative

Objective.To study the incidence of sepsis in the Valencian Community (Spain) during a period of 10 years (1995–2004).Methods.We downloaded data on discharge diagnoses of septicemia in all 26 public hospitals in the Valencian Community during the 10-year study period, as well as the additional discharge diagnoses of each patient.Results.We identified 33,767 cases of sepsis during the study period. The age-standardized incidence rates among men increased from 64.11 (95% confidence interval [CI], 60.37–67.85) cases per 100,000 population in 1995 to 114.02 (95% CI, 109.02–118.50) cases per 100,000 population in 2004 (P < .001), and those among women increased from 45.08 (95% CI, 42.01–48.15) cases per 100,000 population in 1995 to 83.62 (95% CI, 79.85–87.39) cases per 100,000 population in 2004 (P < .001). Gram-negative bacteria were the most frequently involved microorganisms (in 21.4% of cases), and there was a significant increase in the number of sepsis cases caused by these organisms from 1999 onward. The mortality rate was approximately 42.5% among patients hospitalized for sepsis, and mortality was associated with organ failure. In addition, mortality was associated with the microorganism responsible not being known, with infection due to fungi, and with polymicrobial sepsis.Conclusions.The rates of hospitalization both for sepsis overall and for severe sepsis in the Valencian Community (Spain) are lower than those in other countries but are increasing, by 5% each year. The increase in the number of cases in which gram-negative bacteria are the cause of sepsis is notable.

Clinical Experience with Drotrecogin Alfa in Treating Gram-Positive and -Negative Pathogens in Patients with Severe Sepsis

2004 ◽  
Vol 52 (7) ◽  
pp. 470-474 ◽  
Author(s):  
Lawrence A. Cone ◽  
Richard A. Stone ◽  
Kenneth Jesser ◽  
Richard Nelson
Keyword(s):  
Severe Sepsis ◽  
Mortality Rate ◽  
Organ Failure ◽  
Protein C ◽  
Antimicrobial Agents ◽  
Survival Rates ◽  
Activated Protein C ◽  
Gram Positive ◽  
Gram Negative ◽  
Local Disease

BackgroundNewer concepts in the management of severe sepsis and, in particular, in the understanding of the relationship between proinflammatory and procoagulant activities during severe infection have led to the introduction of activated protein C (drotrecogin) into the therapeutic program. The combination of effective antimicrobial therapy, aggressive supportive care, and efforts to antagonize procoagulants and inhibitors of fibrinolysis was used in this study.MethodsWe treated 12 patients with severe sepsis using this combination of antimicrobial agents and drotrecogin. All patients presented with hypotension and organ failure and some with multiple organ failure. Infected patients were separated into those with gram-positive and those with gram-negative infections.ResultsIn contrast to an expected mortality rate of nearly 40% in this group of patients, only 2 (9%) expired. Both deaths were due to infection by gram-negative organisms in patients with complicated abdominal infections and concurrent cancer. All patients with gram-positive organisms survived.ConclusionThose patients with infections caused by gram-positive organisms seemed to have a better prognosis than those with gram-negative infections, perhaps because their illnesses are less complicated by local disease. Although our study is small, it suggests that activated protein C will have a significant beneficial effect on the future treatment of severe sepsis and can reduce the mortality rate significantly. Further improvement in survival rates will require more effective treatment of local disease and associated noninfectious ailments.

scholarly journals Assessing the effect of different shapes of glyco-gold nanoparticles on bacterial adhesion and infections

2015 ◽  
Vol 51 (86) ◽  
pp. 15669-15672 ◽  
Author(s):  
Preeti Madhukar Chaudhary ◽  
Sivakoti Sangabathuni ◽  
Raghavendra Vasudeva Murthy ◽  
Ajay Paul ◽  
Hirekodathakallu V. Thulasiram ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Bacterial Infection ◽  
Bacterial Adhesion ◽  
Different Shapes

Different shapes of glyco gold nanoparticles (AuNPs) tune the bacterial aggregations, which can effectively inhibit the bacterial infection of cells.

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