Monitoring biological effects of 20 nm versus 100 nm silica nanoparticles induced on a human renal cell line using Fourier transform infrared spectroscopy

2016 ◽  
Vol 8 (10) ◽  
pp. 2233-2242 ◽  
Author(s):  
Emmanuelle Barron ◽  
Isabelle Passagne ◽  
Aurélien Auger ◽  
Adrian Travo ◽  
Estelle Rascol ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Ftir Spectroscopy ◽  
Silica Nanoparticles ◽  
Cell Line ◽  
Renal Cell ◽  
Biological Effects ◽  
Renal Cells ◽  
Renal Cell Line ◽  
20 Nm

A method based on FTIR spectroscopy was proposed for monitoring the biological effects induced on human renal cells with SiO2 nanoparticles (NPs).

scholarly journals Different Cytokine and Chemokine Expression Patterns in Malignant Compared to Those in Nonmalignant Renal Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-8
Author(s):  
Nadine Gelbrich ◽  
Hannes Ahrend ◽  
Anne Kaul ◽  
Lars-Ove Brandenburg ◽  
Uwe Zimmermann ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Renal Cell ◽  
Expression Profiles ◽  
Cell Cancer ◽  
Renal Cells ◽  
Chemokine Expression ◽  
Cytokines And Chemokines ◽  
Clinical Biomarkers ◽  
Renal Cell Line

Objective. Cytokines and chemokines are widely involved in cancer cell progression and thus represent promising candidate factors for new biomarkers. Methods. Four renal cell cancer (RCC) cell lines (Caki-1, 786-O, RCC4, and A498) and a nonmalignant renal cell line (RC-124) were examined with respect to their proliferation. The cytokine and chemokine expression pattern was examined by a DNA array (Human Cytokines & Chemokines RT2 Profiler PCR Array; Qiagen, Hilden, Germany), and expression profiles were compared. Results. Caki-1 and 786-O cells exhibited significantly increased proliferation rates, whereas RCC4 and A498 cells demonstrated attenuated proliferation, compared to nonmalignant RC-124 cells. Expression analysis revealed 52 cytokines and chemokines primarily involved in proliferation and inflammation and differentially expressed not only in malignant and nonmalignant renal cells but also in the four RCC cell lines. Conclusion. This is the first study examining the expression of 84 cytokines and chemokines in four RCC cell lines compared to that in a nonmalignant renal cell line. VEGFA, NODAL, and BMP6 correlated with RCC cell line proliferation and, thus, may represent putative clinical biomarkers for RCC progression as well as for RCC diagnosis and prognosis.

scholarly journals The Influence of UV Radiation on the Degradation of Pharmaceutical Formulations Containing Quercetin

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5454
Author(s):  
Iwona Golonka ◽  
Stanisław Wilk ◽  
Witold Musiał
Keyword(s):  
Quality Assurance ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Decay Rate ◽  
Ftir Spectroscopy ◽  
Polyacrylic Acid ◽  
Decomposition Rate ◽  
Pharmaceutical Formulations ◽  
Polymeric Gels ◽  
The Stability

The aim of this study was to assess the photostability of quercetin in the presence of anionic and nonionic polymeric gels with varied compositions of an added component—glycerol. The samples were irradiated continuously at constant temperature. The stability of quercetin in solution and incorporated into the gels was evaluated by an UV-Vis spectrophotometer. FTIR spectroscopy (Fourier-transform infrared spectroscopy) was used to detect the changes in the structure of quercetin depending on the polymer used in the gel, and on the exposure time. Photostabilization is an important aspect of quality assurance in photosensitive compounds. The decomposition rate of quercetin in the ionic preparation of polyacrylic acid (PAA) with glycerol was 1.952·10−3 min−1, whereas the absence of glycerol resulted in a decay rate of 5.032·10−4 min−1. The formulation containing non-ionic methylcellulose resulted in a decomposition rate of quercetin in the range of 1.679·10−3 min−1. The decay rate of quercetin under light influence depended on the composition of the gel. It was found that the cross-linked PAA stabilized quercetin and the addition of glycerol accelerated the photodegradation.

scholarly journals Characterisation Of Polysacharides And Lipids From Selected Green Algae Species By FTIR-ATR Spectroscopy

2015 ◽  
Vol 23 (36) ◽  
pp. 97-102 ◽  
Author(s):  
Alica Bartošová ◽  
Lenka Blinová ◽  
Kristína Gerulová
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Ftir Spectroscopy ◽  
Green Algae ◽  
Spectral Range ◽  
Chlorella Sorokiniana ◽  
Spectral Features ◽  
Fourier Transformed Infrared Spectroscopy ◽  
Atr Spectroscopy ◽  
Chromochloris Zofingiensis

Abstract Fourier transform infrared (FTIR) spectroscopy was used in this study to identify and determine spectral features of Chromochloris zofingiensis (Dönz) Fucíková et L.A. Lewis (SAG 211-14, Gottingen, Germany), Acutodesmus obliguus (Turpin) Hegewald (SAG 276-1, Gottingen, Germany) and Chlorella sorokiniana (K. Brandt) Pröschold et Darienko (SAG 211-40c, Gottingen, Germany). Polysaccharides and lipids from these three algae species were determined using Fourier Transformed Infrared Spectroscopy (FTIR) with ATR accessory with diamante crystal in spectral range from 400 – 4000 cm−1 and resolution 4.

scholarly journals Transport systems for polyamines in the established renal cell line LLC-PK1. Polarized expression of an Na+-dependent transporter

1990 ◽  
Vol 265 (2) ◽  
pp. 609-612 ◽  
Author(s):  
L Van Den Bosch ◽  
H De Smedt ◽  
L Missiaen ◽  
J B Parys ◽  
R Borghgraef
Keyword(s):  
Cell Line ◽  
Renal Cell ◽  
The Other ◽  
Transport Systems ◽  
Present Evidence ◽  
Cell Monolayers ◽  
Other Hand ◽  
Basolateral Side ◽  
Renal Cell Line ◽  
Polyamine Uptake

We present evidence for the existence of an Na(+)-dependent transporter and an Na(+)-independent transporter for polyamines in LLC-PK1 cells. Both transporters could be discriminated by their sensitivity to inhibitors, particularly rho-chloromercuriphenyl sulphate and various polycationic molecules. By using cell monolayers grown on a permeable filter support, we have found that the Na(+)-dependent polyamine uptake occurred preferentially from the basolateral side. The Na(+)-independent uptake, on the other hand, occurred to the same extent from either the apical or the basolateral side.

scholarly journals Integrated Band Intensities of Ethylene (C212H4) by Fourier Transform Infrared Spectroscopy

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
G. B. Lebron ◽  
T. L. Tan
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Ambient Temperature ◽  
Ftir Spectroscopy ◽  
Fourier Transform Infrared ◽  
Vapor Pressures ◽  
Infrared Band ◽  
Combination Bands ◽  
First Time ◽  
Absorbance Spectra

The integrated band intensities of ethylene (12C2H4) in the 640–3260 cm−1 region were determined by Fourier transform infrared (FTIR) spectroscopy. The infrared absorbance spectra of the ν7 and ν10, ν12, ν7+ν8, ν6+ν10, v11, and ν9 and ν2+ν12 bands of ethylene recorded at a resolution of 0.5 cm−1 were measured at an ambient temperature of 296 K at various vapor pressures ranging from 3×10−5 to 1×10−3 atm to obtain respective Beer-Lambert's law plots. The measured integrated band intensities in cm−1/cm atm were S(ν9andν2+ν12)=112.20±0.24, S(ν11)=55.35±0.14, S(ν12)=41.22±0.30, and S(ν7andν10)=328.66±16.55. In addition, the measured infrared band intensities of the ν7+ν8 and ν6+ν10 combination bands of ethylene are reported for the first time: S(ν7+ν8)=21.701±0.028 cm−1/cm atm and S(ν6+ν10)=2.568±0.025 cm−1/cm atm.

Phenothiazine-mediated depolarization of the plasma membrane in a renal cell line

1988 ◽  
Vol 37 (19) ◽  
pp. 3785-3787 ◽  
Author(s):  
James A. Scott ◽  
Alan J. Fischman ◽  
Ban-An Khaw ◽  
Carlos A. Rabito
Keyword(s):  
Plasma Membrane ◽  
Cell Line ◽  
Renal Cell ◽  
Renal Cell Line

Zinc and Cadmium Interactions in a Renal Cell Line Derived from Rabbit Proximal Tubule

2005 ◽  
Vol 99 (3) ◽  
pp. p74-p84 ◽  
Author(s):  
Olivier Barbier ◽  
Angélique Dauby ◽  
Grégory Jacquillet ◽  
Michel Tauc ◽  
Philippe Poujeol ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
Cell Line ◽  
Renal Cell ◽  
Renal Cell Line

Endocytosis of gentamicin in a proximal tubular renal cell line

Life Sciences ◽  
1999 ◽  
Vol 65 (11) ◽  
pp. 1115-1124 ◽  
Author(s):  
Giuliana Decorti ◽  
Noelia Malusà ◽  
Gabriele Furlan ◽  
Luigi Candussio ◽  
Flora Bartoli Klugmann
Keyword(s):  
Cell Line ◽  
Renal Cell ◽  
Proximal Tubular ◽  
Renal Cell Line
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