Application of nucleic acid analogues as receptor layers for biosensors

2016 ◽  
Vol 8 (2) ◽  
pp. 236-244 ◽  
Author(s):  
Agnieszka Bala ◽  
Łukasz Górski
Keyword(s):  
Nucleic Acid ◽  
Clinical Importance ◽  
Rna Fragments ◽  
Nucleic Acid Analogues

Nucleic acid-based biosensors are typically used to detect DNA or RNA fragments of clinical importance.

Structural Rationalization of a Large Difference in RNA Affinity Despite a Small Difference in Chemistry between Two 2‘-O-Modified Nucleic Acid Analogues

2004 ◽  
Vol 126 (46) ◽  
pp. 15006-15007 ◽  
Author(s):  
Rekha Pattanayek ◽  
Latsavongsakda Sethaphong ◽  
Chongle Pan ◽  
Marija Prhavc ◽  
Thazha P. Prakash ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Small Difference ◽  
Nucleic Acid Analogues

Gas-Chromatographic Measurement of Urinary Polyamines in Cancer Patients

1973 ◽  
Vol 19 (8) ◽  
pp. 904-907 ◽  
Author(s):  
M Drue Denton ◽  
Helen S Glazer ◽  
David C Zellner ◽  
Frank G Smith
Keyword(s):  
Nucleic Acid ◽  
Cancer Patients ◽  
Clinical Importance ◽  
Cellular Growth ◽  
Nucleic Acid Structure ◽  
Specificity And Sensitivity ◽  
Chromatographic Procedure ◽  
Chromatographic Measurement ◽  
Urinary Polyamines ◽  
Acid Structure

Abstract The polyamines, spermidine and spermine, and the precursor diamine, putrescine, may have an important role in control of cellular growth because of their interaction with nucleic acid structure, function, and synthesis. We have developed a quantitative gas-chromatographic procedure for measuring polyamines and have measured their urinary excretion by cancer patients and noncancerous patients. Marked variations in values obtained during therapy and in response to some other variables are described. Both hydrolyzed and nonhydrolyzed urine samples have been examined, in order to quantitate both total and free polyamine and putrescine excretions. The potential clinical importance of these studies and the application of other instrumentation such as mass spectroscopy to improve the specificity and sensitivity of this method are discussed.

scholarly journals Plasma Nucleic Acids in the Diagnosis and Management of Malignant Disease

2002 ◽  
Vol 48 (8) ◽  
pp. 1186-1193 ◽  
Author(s):  
Philip J Johnson ◽  
YM Dennis Lo
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Clinical Importance ◽  
Barr Virus ◽  
Circulating Nucleic Acids ◽  
Clinical Relationship ◽  
Malignant State ◽  
Cancer Types ◽  
Epstein Barr ◽  
Near Future

Abstract Background: There is a need for development of molecular markers of cancer that can be used clinically for the detection, prognostication, and monitoring of cancer. Recently, there has been much interest in the potential use of nucleic acid markers in plasma and serum for this purpose. Approach: We reviewed published literature up to 2002 on the topic, with a particular emphasis on reports published between 1996 and 2002. Content: The nucleic acid markers described in plasma and serum include oncogene mutations/amplifications, microsatellite alterations, and gene rearrangements. Such markers have been described in many cancer types, including lung, colon, and breast. Epigenetic alterations, such as aberrant promoter methylation, have been identified in plasma and serum. Viral nucleic acid markers, such as Epstein–Barr virus DNA in plasma and serum, are reviewed in detail with regard to their application to virus-associated cancers such as nasopharyngeal carcinoma and various lymphomas. More recently, mitochondrial DNA and tumor-related mRNAs have been identified in plasma and serum from patients with several types of tumors. Conclusions: Circulating nucleic acids are an emerging class of molecular tumor markers. Their wide applicability and clinical relationship with the malignant state will likely grant them increasing clinical importance in the near future.

RNA interference with 2′,4′-bridged nucleic acid analogues

2010 ◽  
Vol 18 (10) ◽  
pp. 3474-3480 ◽  
Author(s):  
S.M. Abdur Rahman ◽  
Hiroyuki Sato ◽  
Naoto Tsuda ◽  
Sunao Haitani ◽  
Keisuke Narukawa ◽  
...  
Keyword(s):  
Rna Interference ◽  
Nucleic Acid ◽  
Nucleic Acid Analogues

scholarly journals Morpholino Target Molecular Properties Affect the Swelling Process of Oligomorpholino-Functionalized Responsive Hydrogels

Polymers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 268
Author(s):  
Eleonóra Parelius Jonášová ◽  
Bjørn Torger Stokke
Keyword(s):  
Nucleic Acid ◽  
Optical Fibers ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Dna Oligonucleotides ◽  
Morpholino Oligonucleotides ◽  
Responsive Hydrogels ◽  
Nucleic Acid Analogues ◽  
Dna Hydrogels

Responsive hydrogels featuring DNA as a functional unit are attracting increasing interest due to combination of versatility and numerous applications. The possibility to use nucleic acid analogues opens for further customization of the hydrogels. In the present work, the commonly employed DNA oligonucleotides in DNA-co-acrylamide responsive hydrogels are replaced by Morpholino oligonucleotides. The uncharged backbone of this nucleic acid analogue makes it less susceptible to possible enzymatic degradation. In this work we address fundamental issues related to key processes in the hydrogel response; such as partitioning of the free oligonucleotides and the strand displacement process. The hydrogels were prepared at the end of optical fibers for interferometric size monitoring and imaged using confocal laser scanning microscopy of the fluorescently labeled free oligonucleotides to observe their apparent diffusion and accumulation within the hydrogels. Morpholino-based hydrogels’ response to Morpholino targets was compared to DNA hydrogels’ response to DNA targets of the same base-pair sequence. Non-binding targets were observed to be less depleted in Morpholino hydrogels than in DNA hydrogels, due to their electroneutrality, resulting in faster kinetics for Morpholinos. The electroneutrality, however, also led to the total swelling response of the Morpholino hydrogels being smaller than that of DNA, since their lack of charges eliminates swelling resulting from the influx of counter-ions upon oligonucleotide binding. We have shown that employing nucleic acid analogues instead of DNA in hydrogels has a profound effect on the hydrogel response.

ChemInform Abstract: BNAs: Novel Nucleic Acid Analogues with a Bridged Sugar Moiety

ChemInform ◽  
2010 ◽  
Vol 33 (48) ◽  
pp. no-no
Author(s):  
Takeshi Imanishi ◽  
Satoshi Obika
Keyword(s):  
Nucleic Acid ◽  
Sugar Moiety ◽  
Nucleic Acid Analogues

Synthesis and Properties of Nucleic Acid Analogues Consisting of a Benzene—Phosphate Backbone.

ChemInform ◽  
2005 ◽  
Vol 36 (52) ◽  
Author(s):  
Yoshihito Ueno ◽  
Takumi Kato ◽  
Kumiko Sato ◽  
Yasutomo Ito ◽  
Mahito Yoshida ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Phosphate Backbone ◽  
Nucleic Acid Analogues
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