Real-time monitoring AP site incision caused by APE1 using a modified hybridization probe

2016 ◽  
Vol 8 (4) ◽  
pp. 862-868 ◽  
Author(s):  
Bin Liu ◽  
Lan Peng
Keyword(s):  
Real Time ◽  
Sensitivity And Specificity ◽  
High Sensitivity ◽  
Fluorescence Method ◽  
Real Time Monitoring ◽  
Hybridization Probe ◽  
Linear Probe ◽  
Ap Site

A real time fluorescence method with wide promising applications was developed for APE1 assay with high sensitivity and specificity by using a double-stranded linear probe as a substrate and reporter molecule.

scholarly journals Metal Oxide Nanorods-Based Sensor Array for Selective Detection of Biomarker Gases

Sensors ◽  
2021 ◽  
Vol 21 (5) ◽  
pp. 1922
Author(s):  
Gwang Su Kim ◽  
Yumin Park ◽  
Joonchul Shin ◽  
Young Geun Song ◽  
Chong-Yun Kang
Keyword(s):  
Real Time ◽  
Sensor Array ◽  
High Sensitivity ◽  
Gas Analysis ◽  
Diagnostic Methods ◽  
Real Time Monitoring ◽  
Non Invasive ◽  
Sensitivity And Selectivity ◽  
Breath Gas Analysis ◽  
Angle Method

The breath gas analysis through gas phase chemical analysis draws attention in terms of non-invasive and real time monitoring. The array-type sensors are one of the diagnostic methods with high sensitivity and selectivity towards the target gases. Herein, we presented a 2 × 4 sensor array with a micro-heater and ceramic chip. The device is designed in a small size for portability, including the internal eight-channel sensor array. In2O3 NRs and WO3 NRs manufactured through the E-beam evaporator’s glancing angle method were used as sensing materials. Pt, Pd, and Au metal catalysts were decorated for each channel to enhance functionality. The sensor array was measured for the exhaled gas biomarkers CH3COCH3, NO2, and H2S to confirm the respiratory diagnostic performance. Through this operation, the theoretical detection limit was calculated as 1.48 ppb for CH3COCH3, 1.9 ppt for NO2, and 2.47 ppb for H2S. This excellent detection performance indicates that our sensor array detected the CH3COCH3, NO2, and H2S as biomarkers, applying to the breath gas analysis. Our results showed the high potential of the gas sensor array as a non-invasive diagnostic tool that enables real-time monitoring.

scholarly journals Evaluation of the performance of the COVID-19 rapid antigen tests in a tertiary level hospital in Bangladesh.

2021 ◽  
Author(s):  
Mohammad Jahidur Rahman Khan ◽  
Md. Shahadat Hossain ◽  
Samshad Jahan Shumu ◽  
Md. Selim Reza ◽  
Farzana Mim ◽  
...  
Keyword(s):  
Viral Load ◽  
Real Time ◽  
Sensitivity And Specificity ◽  
Detection Rate ◽  
High Sensitivity ◽  
Rt Pcr ◽  
College Hospital ◽  
Medical College ◽  
Qrt Pcr ◽  
Antigen Tests

Abstract Background: While the COVID-19 pandemic is a worldwide crisis, tests with high sensitivity and specificity are essential for identifying and managing COVID-19 patients. Globally, several rapid antigen tests RATs for COVID-19 have been developed, but their clinical efficacy has not been well established. This study aimed to evaluate the performance of several rapid antigen tests (RATs) to diagnose SARS-CoV-2 infection.Methods: This prospective observational study was conducted at Shaheed Suhrawardy Medical College hospital from February 2021 to April 2021 in Dhaka, Bangladesh. This study included the patients admitted in this hospital at the COVID-19 isolation unit or referred from the triage facility of the outdoor department of this hospital suspected as COVID-19 case. Two nasopharyngeal samples were collected simultaneously. one sample was used on the spot for the RAT. The other was sent to the adjacent Shaheed Suhrawardy Medical College COVID-19 RT-PCR laboratory for real-time reverse transcription-polymerase chain reaction (qRT-PCR). The performance of the RAT was evaluated using the results of qRT-PCR as a reference.Results: A total of 223 patients were included in this study, and the real-time RT-PCR detected SARS-CoV-2 in 84 (37.7%) patients. Of these 84 patients, 9 (10.7%) were asymptomatic. The overall sensitivity and specificity of RATs were 78.6% and 99.3%, respectively. The sensitivity was 81.3% in symptomatic cases and 55.6% in asymptomatic cases. False-negatives were observed in 18 patients, 3 of whom were asymptomatic and had a low viral load (cycle threshold (Ct) > 30). The detection rate of RATs was 100% when the Ct value was up to 24. The detection rate was 42.3% when the Ct was >29. The detection rate of RATs was 92.3% when the onset of symptoms was within three days. The detection rate was 33.3% when the onset of symptoms was >7 days.Conclusions: RATs for COVID-19 used in this study delivered an acceptable performance in patients with high viral load and within the first week of the onset of symptoms. They can be used as a supplementary method to RT-PCR for the diagnosis of COVID-19 patients.

Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase

2017 ◽  
Vol 53 (27) ◽  
pp. 3878-3881 ◽  
Author(s):  
Li-Juan Wang ◽  
Zi-Yue Wang ◽  
Qianyi Zhang ◽  
Bo Tang ◽  
Chun-Yang Zhang
Keyword(s):  
Real Time ◽  
Signal Amplification ◽  
Fluorescence Method ◽  
Dna Glycosylase ◽  
Real Time Monitoring ◽  
Thymine Dna Glycosylase ◽  
Dual Signal Amplification

We develop a new fluorescence method for real-time monitoring of thymine DNA glycosylase activity through cyclic enzymatic repairing-mediated dual-signal amplification.

Dissolution kinetics of zinc oxide nanoparticles: real-time monitoring using a Zn2+-specific fluorescent probe

2019 ◽  
Vol 6 (7) ◽  
pp. 2259-2268 ◽  
Author(s):  
Chun Yat Leung ◽  
Yujie Tu ◽  
Ben Zhong Tang ◽  
Wen-Xiong Wang
Keyword(s):  
Zinc Oxide ◽  
Real Time ◽  
Fluorescent Probe ◽  
Zinc Oxide Nanoparticles ◽  
Dissolution Kinetics ◽  
Fluorescence Method ◽  
Oxide Nanoparticles ◽  
Real Time Monitoring ◽  
The Real ◽  
Kinetics Of

A fluorescence method was employed to monitor the real-time dissolution kinetics of zinc oxide nanoparticles.

scholarly journals Comparison of diagnostics of bacterial vaginosis according to clinical signs with results of laboratory investigations

2016 ◽  
Vol 65 (4) ◽  
pp. 76-82
Author(s):  
Elena V. Shipitsina ◽  
Tatyana A. Khusnutdinova ◽  
Olga S. Ryzhkova ◽  
Anna A. Krysanova ◽  
Olga V. Budilovskaya ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Real Time ◽  
Sensitivity And Specificity ◽  
Real Time Pcr ◽  
Vaginal Discharge ◽  
Clinical Signs ◽  
High Sensitivity ◽  
Clinical Diagnostics ◽  
Laboratory Diagnostics ◽  
Molecular Method

Introduction. Bacterial vaginosis (BV) is associated with a number of reproductive health disorders, therefore timely and accurate diagnosis of this condition is exceedingly important. Objective.Comparison of effectiveness of clinical and laboratory diagnostics of BV in women with vaginal discharge. Material and methods. In total, 318 patients addressing gynecological clinics with complaints about vaginal discharge participated in the study. Clinical diagnostics of BV was performed in the clinics participating in patient enrollment in accordance with their clinical practice. For laboratory diagnostics, microscopy of Gram stained smears according to the Nugent method and quantitative real-time PCR were used. Sensitivity and specificity of clinical diagnostics of BV and the molecular method were evaluated using the Nugent method as reference standard. Results. With the Nugent method, BV was diagnosed in 27% of women, with real-time PCR — in 37% of women. Using clinical signs of BV, the condition was diagnosed in 91% women. Sensitivity and specificity of the real-time PCR were 97% and 87%, respectively. Sensitivity of clinical diagnostics was 100%, but specificity was only 17%. Conclusions. Diagnostics of BV based only on the presence of vaginal discharge leads to false positive results and requires laboratory confirmation. The molecular method has a high sensitivity and satisfactory specificity for BV diagnosis and can be used as an alternative to the Nugent method.

scholarly journals Real-time RT-PCR diagnostics of virus causing COVID-19

2020 ◽  
Vol 13 (1) ◽  
pp. 52-63 ◽  
Author(s):  
E. V. Goncharova ◽  
A. E. Donnikov ◽  
V. V. Kadochnikova ◽  
S. A. Morozova ◽  
M. N. Boldyreva ◽  
...  
Keyword(s):  
Real Time ◽  
Sensitivity And Specificity ◽  
High Sensitivity ◽  
Medical Product ◽  
World Health ◽  
Differential Diagnostics ◽  
Clinical Samples ◽  
Rt Pcr ◽  
Study Results ◽  
Pcr Diagnostics

Aim: the study was aimed to develop a reagent kit for the real-time RT-PCR diagnostics of virus causing COVID-19.Materials and Methods. Three target sites were chosen in the genome SARS-CoV-2. The testing included 220 samples, 48 artificially created positive samples (made from patients’ biomaterial) and 172 clinical samples (scrapes from nasal and pharyngeal cavities, bronchoalveolar lavage, expectoration, endotracheal/nasopharyngeal aspirate, feces, post-mortem material), obtained from two medical centers. Preliminary, the obtained biomaterial was analyzed with a reagent kit of comparison. The evaluation was performed with a confidential interval CI 95%. The calculation of CI for the sensitivity and specificity was made based on the distribution of χ2.Results. The authors developed a technology of novel coronavirus infection (COVID-19) real-time RT-PCR diagnostics for the application in practical healthcare and proposed the variants of testing at all the stages (preanalytical, analytical, and post-analytical, including automated results processing). The proposed reagent kit meets the requirements of the World Health Organization and the Ministry of Healthcare of the Russian Federation. The study results demonstrated high sensitivity and specificity. The sensitivity was 100% (95% CI) 95.6–100%; the specificity was 100% (95% CI) 96.7–100%.Conclusion. The proposed reagent kit was registered in the RF as a medical product; the registration certificate No. RZN 2020/9948 dated 01.04.2020. The application of the reagent kit in network laboratories will provide patients with access to testing for the virus causing COVID-19 and contribute to quick differential diagnostics, improvement of pandemic control, and accurate statistics on the spread of the virus. 

scholarly journals Regenerable Bead-Based Microfluidic Device with integrated THIN-Film Photodiodes for Real Time Monitoring of DNA Detection

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 953
Author(s):  
Catarina R. F. Caneira ◽  
Denis R. Santos ◽  
Virginia Chu ◽  
João P. Conde
Keyword(s):  
Thin Film ◽  
Real Time ◽  
Microfluidic Device ◽  
Low Cost ◽  
High Sensitivity ◽  
Significant Loss ◽  
Real Time Monitoring ◽  
Target Dna ◽  
On Chip ◽  
Biosensing System

Nanoporous microbead-based microfluidic systems for biosensing applications allow enhanced sensitivities, while being low cost and amenable for miniaturization. The regeneration of the microfluidic biosensing system results in a further decrease in costs while the integration of on-chip signal transduction enhances portability. Here, we present a regenerable bead-based microfluidic device, with integrated thin-film photodiodes, for real-time monitoring of molecular recognition between a target DNA and complementary DNA (cDNA). High-sensitivity assay cycles could be performed without significant loss of probe DNA density and activity, demonstrating the potential for reusability, portability and reproducibility of the system.

Fluorogenic bidirectional displacement probe-based real-time isothermal DNA amplification and specific visual detection of products

2016 ◽  
Vol 52 (76) ◽  
pp. 11438-11441 ◽  
Author(s):  
Xiong Ding ◽  
Guoping Wang ◽  
Jingjing Sun ◽  
Tao Zhang ◽  
Ying Mu
Keyword(s):  
Real Time ◽  
Sensitivity And Specificity ◽  
Visual Detection ◽  
Dna Amplification ◽  
High Sensitivity

We report an easy-to-design probe as both the primer and the indicator to mediate isothermal DNA amplification with high sensitivity and specificity.

scholarly journals Development of a two-step nucleic acid amplification test for accurate diagnosis of the Mycobacterium tuberculosis complex

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chien-Ru Lin ◽  
Hsin-Yao Wang ◽  
Ting-Wei Lin ◽  
Jang-Jih Lu ◽  
Jason Chia-Hsun Hsieh ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nucleic Acid ◽  
Real Time ◽  
Sensitivity And Specificity ◽  
Real Time Pcr ◽  
Disease Transmission ◽  
High Sensitivity ◽  
Nucleic Acid Amplification ◽  
Amplification Efficiency ◽  
Pcr Assay

AbstractThe Mycobacterium tuberculosis complex (MTBC) remains one of the top 10 leading causes of death globally. The early diagnosis of MTBC can reduce mortality and mitigate disease transmission. However, current nucleic acid amplification diagnostic test methods are generally time-consuming and show suboptimal diagnostic performance, especially in extrapulmonary MTBC samples or acid-fast stain (AFS)-negative cases. Thus, development of an accurate assay for the diagnosis of MTBC is necessary, particularly under the above mentioned conditions. In this study, a single-tube nested real-time PCR assay (N-RTP) was developed and compared with a newly in-house-developed high-sensitivity real-time PCR assay (HS-RTP) using 134 clinical specimens (including 73 pulmonary and 61 extrapulmonary specimens). The amplification efficiency of HS-RTP and N-RTP was 99.8% and 100.7%, respectively. The sensitivity and specificity of HS-RTP and N-RTP for the diagnosis of MTBC in these specimens were 97.5% (77/79) versus 94.9% (75/79) and 80.0% (44/55) versus 89.1% (49/55), respectively. The sensitivity and specificity of HS-RTP and N-RTP for the diagnosis of MTBC in pulmonary specimens were 96.3% (52/54) versus 96.3% (52/54) and 73.7.0% (14/19) versus 89.5% (17/19), respectively; in extrapulmonary specimens, the sensitivity and specificity of HS-RTP and N-RTP were 100% (25/25) versus 92% (23/25) and 83.3% (30/36) versus 88.9% (32/36), respectively. Among the AFS-negative cases, the sensitivity and specificity of HS-RTP and N-RTP were 97.0% (32/33) versus 90.9% (30/33) and 88.0% (44/50) versus 92.0% (46/50), respectively. Overall, the sensitivity of HS-RTP was higher than that of N-RTP, and the performance was not compromised in extrapulmonary specimens and under AFS-negative conditions. In contrast, the specificity of the N-RTP assay was higher than that of the HS-RTP assay in all types of specimens. In conclusion, the HS-RTP assay would be useful for screening patients suspected of exhibiting an MTBC infection due to its higher sensitivity, while the N-RTP assay could be used for confirmation because of its higher specificity. Our results provide a two-step method (screen to confirm) that simultaneously achieves high sensitivity and specificity in the diagnosis of MTBC.

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