Quantification of Zn(ii) using a label-free sensor based on graphene oxide and G-quadruplex

2015 ◽  
Vol 7 (22) ◽  
pp. 9615-9618 ◽  
Author(s):  
Yahui Guo ◽  
Yan Sun ◽  
Xiaoqiang Shen ◽  
Xing Chen ◽  
Weirong Yao ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Assay ◽  
Label Free ◽  
G Quadruplex

A label-free fluorescence assay was developed for the detection of Zn2+ using GO and a guanine-rich sequence H22.

A label-free fluorescent sensor for Pb2+based on G-quadruplex and graphene oxide

2014 ◽  
Vol 6 (20) ◽  
pp. 8120-8123 ◽  
Author(s):  
Yunfeng Bai ◽  
Lu Zhao ◽  
Zezhong Chen ◽  
Haiyan Wang ◽  
Feng Feng
Keyword(s):  
Graphene Oxide ◽  
Fluorescent Sensor ◽  
Label Free ◽  
G Quadruplex

A label-free G-quadruplex-based switch-on fluorescence assay for the selective detection of ATP

The Analyst ◽  
2012 ◽  
Vol 137 (7) ◽  
pp. 1538 ◽  
Author(s):  
Hong-Zhang He ◽  
Victor Pui-Yan Ma ◽  
Ka-Ho Leung ◽  
Daniel Shiu-Hin Chan ◽  
Hui Yang ◽  
...  
Keyword(s):  
Fluorescence Assay ◽  
Selective Detection ◽  
Label Free ◽  
G Quadruplex

A split G-quadruplex DNAzyme based magnetic graphene oxide platform for sensitive authentication of Pseudostellaria heterophylla

RSC Advances ◽  
2016 ◽  
Vol 6 (91) ◽  
pp. 88234-88238
Author(s):  
Z. Z. Zheng ◽  
J. Hu
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Assay ◽  
Its Sequences ◽  
Magnetic Graphene Oxide ◽  
Pseudostellaria Heterophylla ◽  
Magnetic Graphene ◽  
G Quadruplex

Based on the different affinities of graphene oxide (GO) toward ssDNA and dsDNA, a sensitive fluorescence assay utilizing a split G-rich probe and magnetic GO (Fe3O4/GO) was developed for authentication of Pseudostellaria heterophylla based on the ITS sequences.

scholarly journals An Enzyme- and Label-Free Fluorescence Aptasensor for Detection of Thrombin Based on Graphene Oxide and G-Quadruplex

Sensors ◽  
2019 ◽  
Vol 19 (20) ◽  
pp. 4424 ◽  
Author(s):  
Yani Wei ◽  
Luhui Wang ◽  
Yingying Zhang ◽  
Yafei Dong
Keyword(s):  
Graphene Oxide ◽  
Cost Effective ◽  
Target Pair ◽  
Label Free ◽  
Detection Scheme ◽  
G Quadruplex ◽  
Universal Detection ◽  
Dna Strand ◽  
Aptamer Sequence

An enzyme- and label-free aptamer-based assay is described for the determination of thrombin. A DNA strand (S) consisting of two parts was designed, where the first (Sa) is the thrombin-binding aptamer and the second (Se) is a G-quadruplex. In the absence of thrombin, Sa is readily adsorbed by graphene oxide (GO), which has a preference for ss-DNA rather than for ds-DNA. Upon the addition of the N-methyl-mesoporphyrin IX (NMM), its fluorescence (with excitation/emission at 399/610 nm) is quenched by GO. In contrast, in the presence of thrombin, the aptamer will bind thrombin, and thus, be separated from GO. As a result, fluorescence will be enhanced. The increase is linear in the 0.37 µM to 50 µM thrombin concentration range, and the detection limit is 0.37 nM. The method is highly selective over other proteins, cost-effective, and simple. In our perception, it represents a universal detection scheme that may be applied to other targets according to the proper choice of the aptamer sequence and formation of a suitable aptamer-target pair.

A label-free fluorescence assay for potassium ions using riboflavin as a G-quadruplex ligand

The Analyst ◽  
2015 ◽  
Vol 140 (10) ◽  
pp. 3352-3355 ◽  
Author(s):  
Lijun Xu ◽  
Na Sun ◽  
Lu Zhou ◽  
Xing Chen ◽  
Jine Wang ◽  
...  
Keyword(s):  
Fluorescence Assay ◽  
Potassium Ions ◽  
Label Free ◽  
G Quadruplex

A label-free fluorescence K+assay was developed using riboflavin, a new G-quadruplex ligand, and a G-quadruplex sequence (PW17).

scholarly journals Label-Free G-Quadruplex Aptamer Fluorescence Assay for Ochratoxin A Using a Thioflavin T Probe

Toxins ◽  
2018 ◽  
Vol 10 (5) ◽  
pp. 198 ◽  
Author(s):  
Kefeng Wu ◽  
Changbei Ma ◽  
Han Zhao ◽  
Hailun He ◽  
Hanchun Chen
Keyword(s):  
Ochratoxin A ◽  
Fluorescence Assay ◽  
Thioflavin T ◽  
Label Free ◽  
G Quadruplex

Label-free fluorescence assay for rapid detection of RNase H activity based on Tb3+-induced G-quadruplex conjugates

2017 ◽  
Vol 9 (20) ◽  
pp. 3055-3060 ◽  
Author(s):  
Kefeng Wu ◽  
Changbei Ma ◽  
Haisheng Liu ◽  
Hailun He ◽  
Weimin Zeng ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Rnase H ◽  
Fluorescence Assay ◽  
Label Free ◽  
Label Free Detection ◽  
G Quadruplex

A simple fluorescence based biosensor for label-free detection of RNase H activity based on Tb3+-induced G-quadruplex conjugates is reported.

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