Tissue distribution of 10-methoxycamptothecin and its metabolite 10-hydroxycamptothecin in rats by a RP-HPLC method with fluorescence detection/UV detection

2015 ◽  
Vol 7 (18) ◽  
pp. 7690-7696 ◽  
Author(s):  
Changmin Shao ◽  
Jian Zheng ◽  
Bo Fan ◽  
Shaman Luo ◽  
Jixiang Lin ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Tissue Distribution ◽  
Fluorescence Detection ◽  
Broad Spectrum ◽  
Hplc Method ◽  
Uv Detection ◽  
Camptotheca Acuminata ◽  
Rp Hplc ◽  
Derivatives Of

Both 10-methoxycamptothecin (MCPT) and 10-hydroxycamptothecin (HCPT) are natural derivatives of camptothecin (CPT) isolated from Camptotheca acuminata, possessing broad-spectrum antitumor activity.

Development of validated RP HPLC method with fluorescence detection for simultaneous quantification of sacubitril and valsartan from rat plasma

2018 ◽  
Vol 41 (5) ◽  
pp. 246-252 ◽  
Author(s):  
Mahesh Attimarad ◽  
Sree Harsha Nagaraja ◽  
Anroop Balachandran Nair ◽  
Bandar Essa Aldhubaib ◽  
Venugopala Narayanaswamy Katharigatta
Keyword(s):  
Fluorescence Detection ◽  
Rat Plasma ◽  
Hplc Method ◽  
Simultaneous Quantification ◽  
Rp Hplc

RP-HPLC method with fluorescence detection for amino acids D /L ratio determination in fossil bones as a marker of DNA preservation

2010 ◽  
Vol 33 (16) ◽  
pp. 2411-2416 ◽  
Author(s):  
Piera Iuliani ◽  
Lucia Di Federico ◽  
Gabriella Fontecchio ◽  
Giuseppe Carlucci
Keyword(s):  
Amino Acids ◽  
Fluorescence Detection ◽  
Hplc Method ◽  
Dna Preservation ◽  
Rp Hplc ◽  
Fossil Bones ◽  
Ratio Determination

Molecular and Quantum Mechanical Study for the Separation of Cefprozil in the Presence of Its Alkaline Degradation Product Using RP-HPLC with UV Detection

2017 ◽  
Vol 100 (2) ◽  
pp. 392-399 ◽  
Author(s):  
Khalid A M Attia ◽  
Mohammed W I Nassar ◽  
Mohamed B El-Zeiny ◽  
Ahmed Serag
Keyword(s):  
Degradation Product ◽  
Generation Cephalosporin ◽  
Reversed Phase ◽  
Hplc Method ◽  
Uv Detection ◽  
Quantum Mechanical ◽  
Rp Hplc ◽  
Quantum Mechanical Study ◽  
Alkaline Conditions

Abstract A reversed-phase HPLC method (RP-HPLC) with UV detection was developed and validated for the quantitative determination of cefprozil, a second-generation cephalosporin. Due to β-lactam ring instability under alkaline conditions, this RP-HPLC method was applied for the determination of cefprozil in the presence of its possible degradation product. The interactions that govern the separation process with stationary phase were investigated at both molecular and quantum mechanical levels. Moreover, electrostatic potential maps were generated to determine the sites of interaction with mobile phase. The suggested method was validated in compliance with International Conference on Harmonization guidelines and successfully applied for the determination of cefprozil in its commercial pharmaceutical formulation.

New RP‐HPLC Method with UV‐Detection for the Determination of Carvedilol in Human Serum

2007 ◽  
Vol 30 (11) ◽  
pp. 1677-1685 ◽  
Author(s):  
Ramesh Gannu ◽  
Vamshi Vishnu Yamsani ◽  
Yamsani Madhusudan Rao
Keyword(s):  
Human Serum ◽  
Hplc Method ◽  
Uv Detection ◽  
Rp Hplc

Dietary salicylates in herbs and spices

2019 ◽  
Vol 10 (11) ◽  
pp. 7037-7041 ◽  
Author(s):  
Danuta Gajewska ◽  
Paulina Katarzyna Kęszycka ◽  
Michał Szkop
Keyword(s):  
Fluorescence Detection ◽  
Hplc Method ◽  
Rp Hplc ◽  
Culinary Herbs ◽  
Herbs And Spices

The aim of this study was to determine dietary salicylate content in selected culinary herbs and spices, using the RP-HPLC method with fluorescence detection.

scholarly journals Optimization of RP-HPLC method with UV detection for determination of ursodeoxycholic acid in pharmaceutical formulations

2020 ◽  
Vol 66 (1) ◽  
pp. 85-90
Author(s):  
Zhaklina Poposka Svirkova ◽  
Zorica Arsova-Sarafinovska ◽  
Aleksandra Grozdanova
Keyword(s):  
Ursodeoxycholic Acid ◽  
Flow Rate ◽  
Mobile Phase ◽  
Pharmaceutical Formulations ◽  
Gradient Elution ◽  
Hplc Method ◽  
Uv Detection ◽  
Rp Hplc ◽  
Ich Guidelines

Due to the low absorptivity of bile acids, the aim of this study was to develop and validate a simple and sensitive HPLC/UV method for quantification of ursodeoxycholic acid (UDCA) in pharmaceutical formulations. Effective separation was achieved on C18 end–capped column, with gradient elution of a mobile phase composed of 0.001 M phosphate buffer (pH 2.8±0.5) – acetonitrile mix, at flow rate 1.5 mL min-1, UV detection at 200 nm and injection volumes were 50 µL. The proposed HPLC method was fully validated according to the ICH guidelines and it was found to be simple, accurate, precise and robust. Key words: ursodeoxycholic acid, HPLC/UV, pharmaceutical formulations, validation

DEVELOPMENT OF A VALIDATED RP-HPLC METHOD FOR ESTIMATION OF DARUNAVIR IN SPIKED HUMAN PLASMA WITH UV DETECTION

2016 ◽  
Vol 7 (1) ◽  
pp. 24-28 ◽  
Author(s):  
Zakir Ahmad ◽  
Sandeep S Sonawane ◽  
Atharva S Bhalerao ◽  
Sanjay J Kshirsagar
Keyword(s):  
Human Plasma ◽  
Hplc Method ◽  
Uv Detection ◽  
Spiked Human Plasma ◽  
Rp Hplc
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