A highly sensitive strategy for base excision repair enzyme activity detection based on graphene oxide mediated fluorescence quenching and hybridization chain reaction

The Analyst ◽  
2016 ◽  
Vol 141 (1) ◽  
pp. 96-99 ◽  
Author(s):  
Qiang Xi ◽  
Jun-Jie Li ◽  
Wen-Fang Du ◽  
Ru-Qin Yu ◽  
Jian-Hui Jiang
Keyword(s):  
Enzyme Activity ◽  
Fluorescence Quenching ◽  
Base Excision Repair ◽  
Excision Repair ◽  
Hybridization Chain Reaction ◽  
Activity Detection ◽  
Chain Reaction ◽  
Repair Enzyme ◽  
Highly Sensitive ◽  
Base Excision

We report a highly sensitive strategy for UDG activity detection by combining HCR amplification and a GO-based fluorescence quenching platform.

Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe

2013 ◽  
Vol 49 (50) ◽  
pp. 5630 ◽  
Author(s):  
Ka-Ho Leung ◽  
Hong-Zhang He ◽  
Victor Pui-Yan Ma ◽  
Hai-Jing Zhong ◽  
Daniel Shiu-Hin Chan ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Base Excision Repair ◽  
Excision Repair ◽  
Repair Enzyme ◽  
G Quadruplex ◽  
Base Excision

A fluorescent G-quadruplex probe for the assay of base excision repair enzyme activity

2015 ◽  
Vol 51 (72) ◽  
pp. 13744-13747 ◽  
Author(s):  
Chang Yeol Lee ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Enzyme Activity ◽  
Base Excision Repair ◽  
Fluorescence Enhancement ◽  
Excision Repair ◽  
High Sensitivity ◽  
Repair Enzyme ◽  
Quadruplex Structure ◽  
G Quadruplex ◽  
Base Excision ◽  
Novel Strategy

A G-quadruplex probe incorporating 2-AP is utilized to develop a novel strategy to accurately determine UDG activity. The excision reaction promoted by UDG is designed to trigger the formation of G-quadruplex structure with significant fluorescence enhancement of 2-AP within the probe. By employing this strategy, UDG activity can be reliably determined with high sensitivity and specificity.

DNA-mediated supercharged fluorescent protein/graphene oxide interaction for label-free fluorescence assay of base excision repair enzyme activity

2015 ◽  
Vol 51 (69) ◽  
pp. 13373-13376 ◽  
Author(s):  
Zhen Wang ◽  
Yong Li ◽  
Lijun Li ◽  
Daiqi Li ◽  
Yan Huang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Graphene Oxide ◽  
Base Excision Repair ◽  
Fluorescent Protein ◽  
Excision Repair ◽  
Label Free ◽  
Repair Enzyme ◽  
Quenching Effect ◽  
Base Excision ◽  
Green Fluorescent

The interaction between supercharged green fluorescent protein (ScGFP) and graphene oxide (GO) as well as the resulting quenching effect of GO on ScGFP were investigated.

scholarly journals Base excision repair enzyme endonuclease III suppresses mutagenesis caused by 8-hydroxy-dGTP

DNA Repair ◽  
2008 ◽  
Vol 7 (1) ◽  
pp. 88-94 ◽  
Author(s):  
Tetsuya Suzuki ◽  
Kazuo Yamamoto ◽  
Hideyoshi Harashima ◽  
Hiroyuki Kamiya
Keyword(s):  
Base Excision Repair ◽  
Excision Repair ◽  
Repair Enzyme ◽  
Endonuclease Iii ◽  
Base Excision
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