A target-activated autocatalytic DNAzyme amplification strategy for the assay of base excision repair enzyme activity

2012 ◽  
Vol 48 (70) ◽  
pp. 8820 ◽  
Author(s):  
Liangliang Zhang ◽  
Jingjin Zhao ◽  
Jianhui Jiang ◽  
Ruqin Yu
Keyword(s):  
Enzyme Activity ◽  
Base Excision Repair ◽  
Excision Repair ◽  
Repair Enzyme ◽  
Base Excision ◽  
Amplification Strategy

Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe

2013 ◽  
Vol 49 (50) ◽  
pp. 5630 ◽  
Author(s):  
Ka-Ho Leung ◽  
Hong-Zhang He ◽  
Victor Pui-Yan Ma ◽  
Hai-Jing Zhong ◽  
Daniel Shiu-Hin Chan ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Base Excision Repair ◽  
Excision Repair ◽  
Repair Enzyme ◽  
G Quadruplex ◽  
Base Excision

A fluorescent G-quadruplex probe for the assay of base excision repair enzyme activity

2015 ◽  
Vol 51 (72) ◽  
pp. 13744-13747 ◽  
Author(s):  
Chang Yeol Lee ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Enzyme Activity ◽  
Base Excision Repair ◽  
Fluorescence Enhancement ◽  
Excision Repair ◽  
High Sensitivity ◽  
Repair Enzyme ◽  
Quadruplex Structure ◽  
G Quadruplex ◽  
Base Excision ◽  
Novel Strategy

A G-quadruplex probe incorporating 2-AP is utilized to develop a novel strategy to accurately determine UDG activity. The excision reaction promoted by UDG is designed to trigger the formation of G-quadruplex structure with significant fluorescence enhancement of 2-AP within the probe. By employing this strategy, UDG activity can be reliably determined with high sensitivity and specificity.

DNA-mediated supercharged fluorescent protein/graphene oxide interaction for label-free fluorescence assay of base excision repair enzyme activity

2015 ◽  
Vol 51 (69) ◽  
pp. 13373-13376 ◽  
Author(s):  
Zhen Wang ◽  
Yong Li ◽  
Lijun Li ◽  
Daiqi Li ◽  
Yan Huang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Graphene Oxide ◽  
Base Excision Repair ◽  
Fluorescent Protein ◽  
Excision Repair ◽  
Label Free ◽  
Repair Enzyme ◽  
Quenching Effect ◽  
Base Excision ◽  
Green Fluorescent

The interaction between supercharged green fluorescent protein (ScGFP) and graphene oxide (GO) as well as the resulting quenching effect of GO on ScGFP were investigated.

A highly sensitive strategy for base excision repair enzyme activity detection based on graphene oxide mediated fluorescence quenching and hybridization chain reaction

The Analyst ◽  
2016 ◽  
Vol 141 (1) ◽  
pp. 96-99 ◽  
Author(s):  
Qiang Xi ◽  
Jun-Jie Li ◽  
Wen-Fang Du ◽  
Ru-Qin Yu ◽  
Jian-Hui Jiang
Keyword(s):  
Enzyme Activity ◽  
Fluorescence Quenching ◽  
Base Excision Repair ◽  
Excision Repair ◽  
Hybridization Chain Reaction ◽  
Activity Detection ◽  
Chain Reaction ◽  
Repair Enzyme ◽  
Highly Sensitive ◽  
Base Excision

We report a highly sensitive strategy for UDG activity detection by combining HCR amplification and a GO-based fluorescence quenching platform.

scholarly journals Base excision repair enzyme endonuclease III suppresses mutagenesis caused by 8-hydroxy-dGTP

DNA Repair ◽  
2008 ◽  
Vol 7 (1) ◽  
pp. 88-94 ◽  
Author(s):  
Tetsuya Suzuki ◽  
Kazuo Yamamoto ◽  
Hideyoshi Harashima ◽  
Hiroyuki Kamiya
Keyword(s):  
Base Excision Repair ◽  
Excision Repair ◽  
Repair Enzyme ◽  
Endonuclease Iii ◽  
Base Excision
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