Colorimetric detection of biological hydrogen sulfide using fluorosurfactant functionalized gold nanorods

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7443-7450 ◽  
Author(s):  
Xuan Zhang ◽  
Wenjuan Zhou ◽  
Zhiqin Yuan ◽  
Chao Lu
Keyword(s):  
Hydrogen Sulfide ◽  
Gold Nanorods ◽  
Colorimetric Detection ◽  
Mouse Serum ◽  
Serum Samples ◽  
Human And Mouse

Fluorosurfactant functionalized gold nanorods can act as selective colorimetric nanoprobes for H2S. These proposed nanoprobes can accurately detect biological H2S in human and mouse serum samples as well evaluate the activity of H2S synthetase.

Selective Colorimetric Detection of Hydrogen Sulfide Based on Primary Amine-Active Ester Cross-Linking of Gold Nanoparticles

2015 ◽  
Vol 87 (14) ◽  
pp. 7267-7273 ◽  
Author(s):  
Zhiqin Yuan ◽  
Fengniu Lu ◽  
Meihua Peng ◽  
Chia-Wei Wang ◽  
Yu-Ting Tseng ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Hydrogen Sulfide ◽  
Primary Amine ◽  
Colorimetric Detection ◽  
Cross Linking ◽  
Active Ester

scholarly journals Detection of circulating antigens in serum samples of mice experimentally infected with Trichinella spiralis by a sandwich ELISA based on IgY

2014 ◽  
Vol 51 (3) ◽  
pp. 181-189 ◽  
Author(s):  
F. Jing ◽  
J. Cui ◽  
R. Liu ◽  
L. Liu ◽  
P. Jiang ◽  
...  
Keyword(s):  
Post Infection ◽  
Trichinella Spiralis ◽  
Sandwich Elisa ◽  
Egg Yolk ◽  
Mouse Serum ◽  
Serum Samples ◽  
Egg Yolk Immunoglobulin ◽  
Muscle Larvae ◽  
Sandwich Elisa Assay ◽  
Circulating Antigens

AbstractIn the present study, a sandwich ELISA based on IgY (egg yolk immunoglobulin) was developed for detection of circulating antigens (CAg) in sere of mice experimentally infected with Trichinella spiralis. The IgY-sandwich ELISA assay involved the use of chicken antibody IgY against excretory-secretory (ES) antigens of Trichinella spiralis muscle larvae as a capture antibody and mouse polyclonal antibody IgG to ES antigens as a detecting antibody. This method was able to detect as little as 3 ng/ml of ES antigens added to normal mouse serum. A group of sixteen mice was orally inoculated with 500 T. spiralis muscle larvae per animal. The serum samples from the infected mice were taken during 1–35 days post-infection (dpi). The CAg was detectable as early as 8 dpi in the sera of infected mice. The level of CAg increased dramatically during 13–15 dpi and reached a peak at 22 dpi and remained a plateau for 3 days, then declined gradually. Another peak of CAg occurred at 31 dpi. The anti-Trichinella antibodies was first detected in 14.3 % of the infected mice at 2 weeks post-infection (wpi), and reached a peak positive rate of 100 % at 5 wpi. Moreover, the infected mice were treated with abendazole at 5 wpi and the serum CAg levels increased significantly during 2–6 days posttreatment (dpt) and then declined rapidly during 8–14 dpt. By 42 dpt, the CAg levels decreased to the undetected level, but the detection rate of antibodies was still 100 %. The IgY-sandwich ELISA appears to be a sensitive for detection of antigenemia of T. spiralis and valuable to judge the efficacy of chemotherapy in trichinellosis.

scholarly journals Colorimetric Detection of Hydrogen Sulfide in Ambient Air

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 804
Author(s):  
Laura Engel ◽  
Karina R. Tarantik ◽  
Carolin Pannek ◽  
Jürgen Wöllenstein
Keyword(s):  
Hydrogen Sulfide ◽  
Azo Dye ◽  
Screen Printing ◽  
Color Change ◽  
Colorimetric Detection ◽  
Ambient Air ◽  
Fast Method ◽  
Vis Spectroscopy

We present a fast method to monitor hydrogen sulfide (H2S) in ambient air based on a visible color change. Therefore, an immobilized copper(II) complex of the azo dye 1-(2-pyridylazo)-2-naphtol (H-PAN) was synthesized and prepared in a matrix for screen printing. Different materials, reaching from opaque paper to transparent foils served as substrate. The reaction of the copper(II) complex (Cu-PAN) to the target gas H2S was measured in reflection via UV/VIS spectroscopy.

Development of an HPLC method for the determination of doripenem in human and mouse serum

2007 ◽  
Vol 853 (1-2) ◽  
pp. 123-126 ◽  
Author(s):  
Christina Sutherland ◽  
David P. Nicolau
Keyword(s):  
Hplc Method ◽  
Mouse Serum ◽  
Human And Mouse

Comparing the applicability of CGE-on-the-chip and SDS-PAGE for fast pre-screening of mouse serum samples prior to proteomics analysis

2008 ◽  
Vol 29 (21) ◽  
pp. 4332-4340 ◽  
Author(s):  
Tom Grunert ◽  
Martina Marchetti-Deschmann ◽  
Ingrid Miller ◽  
Mathias Müller ◽  
Günter Allmaier
Keyword(s):  
Mouse Serum ◽  
Serum Samples ◽  
Proteomics Analysis ◽  
Sds Page

scholarly journals Estimation of Estradiol in Mouse Serum Samples: Evaluation of Commercial Estradiol Immunoassays

Endocrinology ◽  
2011 ◽  
Vol 152 (11) ◽  
pp. 4443-4447 ◽  
Author(s):  
Daniel J. Haisenleder ◽  
Aleisha H. Schoenfelder ◽  
Elizabeth S. Marcinko ◽  
Lisa M. Geddis ◽  
John C. Marshall
Keyword(s):  
Enzyme Immunoassay ◽  
Standard Method ◽  
Gold Standard ◽  
Correlation Study ◽  
Sample Volume ◽  
Mouse Serum ◽  
Serum Samples ◽  
Gold Standard Method ◽  
Standard Curve ◽  
Minimal Sample

The University of Virginia Center for Research in Reproduction Ligand Core performed an evaluation of nine commercial estradiol (E2) immunoassays for use with mouse serum. The evaluation had two components. 1) Recovery Studies: a mouse pool was spiked with E2 concentrations across the assay range, and percent recovery and parallelism to the assay standard curve were determined. 2) Correlation Studies: serum pools were collected from intact females, ovariectomized (OVX) and OVX-E2 treated mice and E2 assayed, then measured by gas chromatography/tandem mass spectrometry (GC/MSMS) for comparison to a gold standard method. Recovery results showed that E2 recovery from spiked mouse pools varied greatly (from <18% to >640%) among kits tested. However, three kits (DiaSorin Radioimmunoassay, Siemens Double Antibody RIA, and CalBiotech Enzyme Immunoassay) showed reasonable recoveries and parallelism. Data collected from the Correlation Study showed that values from intact, OVX and OVX-E2-treated mouse pools varied by several fold vs. GC/MSMS for most of the kits tested. The DiaSorin RIA and CalBiotech Enzyme Immunoassay Kits showed the best correlation to GC/MSMS. Unfortunately, while this evaluation was ongoing, the DiaSorin Kit was discontinued. In summary, the CalBiotech Kit was the only available assay tested that demonstrated good E2 parallelism to the assay standard curve and accuracy vs. a gold standard method (i.e. GC/MSMS). Also of note, the CalBiotech assay is sensitive and requires minimal sample volume. Therefore, based on these findings the CalBiotech E2 assay has been implemented for use in mouse serum samples within the Ligand Core.

scholarly journals Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase

2015 ◽  
Vol 7 (50) ◽  
pp. 27639-27645 ◽  
Author(s):  
Zhiyang Zhang ◽  
Zhaopeng Chen ◽  
Shasha Wang ◽  
Fangbin Cheng ◽  
Lingxin Chen
Keyword(s):  
Alkaline Phosphatase ◽  
Gold Nanorods ◽  
Colorimetric Detection
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