scholarly journals Iodine-Mediated Etching of Gold Nanorods for Plasmonic ELISA Based on Colorimetric Detection of Alkaline Phosphatase

2015 ◽  
Vol 7 (50) ◽  
pp. 27639-27645 ◽  
Author(s):  
Zhiyang Zhang ◽  
Zhaopeng Chen ◽  
Shasha Wang ◽  
Fangbin Cheng ◽  
Lingxin Chen
Keyword(s):  
Alkaline Phosphatase ◽  
Gold Nanorods ◽  
Colorimetric Detection

Colorimetric detection of biological hydrogen sulfide using fluorosurfactant functionalized gold nanorods

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7443-7450 ◽  
Author(s):  
Xuan Zhang ◽  
Wenjuan Zhou ◽  
Zhiqin Yuan ◽  
Chao Lu
Keyword(s):  
Hydrogen Sulfide ◽  
Gold Nanorods ◽  
Colorimetric Detection ◽  
Mouse Serum ◽  
Serum Samples ◽  
Human And Mouse

Fluorosurfactant functionalized gold nanorods can act as selective colorimetric nanoprobes for H2S. These proposed nanoprobes can accurately detect biological H2S in human and mouse serum samples as well evaluate the activity of H2S synthetase.

Sensitive, colorimetric enzyme amplification cascade for determination of alkaline phosphatase and application of the method to an immunoassay of thyrotropin

1991 ◽  
Vol 37 (9) ◽  
pp. 1513-1518 ◽  
Author(s):  
D M Obzansky ◽  
B R Rabin ◽  
D M Simons ◽  
S Y Tseng ◽  
D M Severino ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Colorimetric Detection ◽  
High Sensitivity ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Highly Sensitive ◽  
Wide Range ◽  
Colored Product ◽  
Enzyme Amplification ◽  
Amplification Cascade

Abstract A highly sensitive flavin adenine dinucleotide-3'-phosphate (FADP)-based enzyme amplification cascade has been developed for determining alkaline phosphatase (ALP; EC 3.1.3.1). The cascade detects ALP via the dephosphorylation of the novel substrate FADP to produce the cofactor FAD, which binds stoichiometrically to inactive apo D-amino acid oxidase (D-AAO). The resulting active holo D-AAO oxidizes D-proline to produce hydrogen peroxide, which is quantified by the horseradish peroxidase-mediated conversion of 3,5-dichloro-2-hydroxybenzenesulfonic acid and 4-aminoantipyrine to a colored product. The FADP-based enzyme amplification cascade has been used in a novel releasable linker immunoassay (RELIA) to quantify thyrotropin (TSH). In the assay, TSH is first captured onto antibody-coated chromium dioxide particles. After formation of an antibody-TSH sandwich with a dethiobiotinylated second antibody, the complex is reacted with a streptavidin-ALP conjugate. Biotin is then used to release the conjugate into solution, and ALP is quantified in an automated version of the FADP-based amplification cascade on the aca discrete clinical analyzer (Du Pont). The sensitivity of the colorimetric RELIA assay for TSH (less than 0.1 milli-int. unit/L) is comparable with that of fluorometric assays. This technology provides a way to adapt to the aca high-sensitivity immunoassays for a wide range of analytes via colorimetric detection.

Multicolor ELISA based on alkaline phosphatase-triggered growth of Au nanorods

The Analyst ◽  
2016 ◽  
Vol 141 (10) ◽  
pp. 2970-2976 ◽  
Author(s):  
Yanyan Li ◽  
Xiaoming Ma ◽  
Zhengming Xu ◽  
Meihua Liu ◽  
Zhenyu Lin ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Gold Nanorods ◽  
Au Nanorods ◽  
Seed Mediated ◽  
First Time

In this work, seed-mediated synthesis of gold nanorods (AuNRs) is demonstrated for multicolor biosensing for the first time.

scholarly journals Blocked Enzymatic Etching of Gold Nanorods: Application to Colorimetric Detection of Acetylcholinesterase Activity and Its Inhibitors

2016 ◽  
Vol 8 (17) ◽  
pp. 11139-11146 ◽  
Author(s):  
Laura Saa ◽  
Ruta Grinyte ◽  
Ana Sánchez-Iglesias ◽  
Luis M. Liz-Marzán ◽  
Valeri Pavlov
Keyword(s):  
Gold Nanorods ◽  
Colorimetric Detection ◽  
Acetylcholinesterase Activity

High-resolution colorimetric detection of lipase activity based on enzyme-controlled reshaping of gold nanorods

2019 ◽  
Vol 11 (17) ◽  
pp. 2286-2291 ◽  
Author(s):  
Hao Zhang ◽  
Shengnan Wu ◽  
Linghua Zhang ◽  
Ling Jiang ◽  
Fengwei Huo ◽  
...  
Keyword(s):  
High Resolution ◽  
Gold Nanorods ◽  
Lipase Activity ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Sensing Mechanism

The sensing mechanism of a high resolution colorimetric assay for the visual readout of lipase activity based on AuNR reshaping.

State-of-the-art strategies for the colorimetric detection of heavy metals using gold nanorods based on aspect ratio reduction

2016 ◽  
Vol 8 (10) ◽  
pp. 2131-2137 ◽  
Author(s):  
Sruthi Ann Alex ◽  
N. Chandrasekaran ◽  
Amitava Mukherjee
Keyword(s):  
Heavy Metals ◽  
Aspect Ratio ◽  
Gold Nanorods ◽  
State Of The Art ◽  
Colorimetric Detection ◽  
Detection Techniques ◽  
Ratio Reduction

Current colorimetric detection techniques for metals using gold nanorods based on variations in the aspect ratio have been summarized.

Colorimetric Detection of Dopamine Based on Iodine-mediated Etching of Gold Nanorods

2021 ◽  
Vol 49 (1) ◽  
pp. 60-67
Author(s):  
Xiu QIN ◽  
Chun-Ling YUAN ◽  
Rui SHI ◽  
Shu-Zheng WANG ◽  
Yi-Lin WANG
Keyword(s):  
Gold Nanorods ◽  
Colorimetric Detection
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