A versatile and highly sensitive homogeneous electrochemical strategy based on the split aptamer binding-induced DNA three-way junction and exonuclease III-assisted target recycling

The Analyst ◽  
2015 ◽  
Vol 140 (16) ◽  
pp. 5748-5753 ◽  
Author(s):  
Ting Hou ◽  
Wei Li ◽  
Lianfang Zhang ◽  
Feng Li
Keyword(s):  
Exonuclease Iii ◽  
Target Recycling ◽  
Atp Assay ◽  
Electrochemical Biosensing ◽  
Highly Sensitive ◽  
Junction Formation ◽  
Exo Iii

A versatile and highly sensitive homogeneous electrochemical biosensing platform has been developed for an ATP assay based on split aptamer binding-induced DNA three-way junction formation and Exo III-assisted target recycling.

Highly sensitive detection of nucleic acids using a cascade amplification strategy based on exonuclease III-assisted target recycling and conjugated polyelectrolytes

The Analyst ◽  
2018 ◽  
Vol 143 (18) ◽  
pp. 4267-4272 ◽  
Author(s):  
Biqing Bao ◽  
Yanrui Pan ◽  
Bingbing Gu ◽  
Jia Chen ◽  
Yu Xu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Conjugated Polyelectrolytes ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Exo Iii ◽  
Highly Sensitive Detection ◽  
Amplification Strategy ◽  
Cascade Amplification

A ratiometric and cascade amplification strategy that combines the signal amplification and effecitive FRET property of CPEs with the Exo III-assisted target recycling method has been developed for DNA detection.

Label-free DNA detection based on oligonucleotide-stabilized silver nanoclusters and exonuclease III-catalyzed target recycling amplification

2014 ◽  
Vol 6 (15) ◽  
pp. 6082-6087 ◽  
Author(s):  
Hui Ma ◽  
Wei Wei ◽  
Qian Lu ◽  
Zhixin Zhou ◽  
Henan Li ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Free Dna ◽  
Sensitivity And Selectivity ◽  
Exo Iii ◽  
Ag Ncs ◽  
Recycling Amplification

A label-free DNA biosensor with high sensitivity and selectivity is constructed by using DNA–Ag NCs and Exo III-catalyzed target recycling amplification.

Ultrasensitive electrochemical detection of Hg2+based on an Hg2+-triggered exonuclease III-assisted target recycling strategy

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5771-5778 ◽  
Author(s):  
Xiaolei Song ◽  
Yu Wang ◽  
Su Liu ◽  
Xue Zhang ◽  
Haiwang Wang ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Electrochemical Method ◽  
Sensitive Detection ◽  
Mercury Ions ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Recycling Amplification ◽  
Highly Sensitive Detection

An isothermal electrochemical method for the highly sensitive detection of mercury ions (Hg2+) was established based on Hg2+-triggered exonuclease III-aided target recycling amplification.

A ratiometric electrochemical biosensor for ultrasensitive and highly selective detection of the K-ras gene via exonuclease III-assisted target recycling and rolling circle amplification strategies

2019 ◽  
Vol 11 (32) ◽  
pp. 4146-4156 ◽  
Author(s):  
Qi Xiao ◽  
Jinrong Feng ◽  
Jiawen Li ◽  
Yi Liu ◽  
Dan Wang ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Selective Detection ◽  
Ras Gene ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Rolling Circle ◽  
Exo Iii

A ratiometric electrochemical biosensor for ultrasensitive and highly selective detection of the K-ras gene via Exo III-assisted target recycling and RCA strategies.

Homogeneous electrochemical aptamer-based ATP assay with signal amplification by exonuclease III assisted target recycling

2013 ◽  
Vol 49 (23) ◽  
pp. 2335 ◽  
Author(s):  
Shufeng Liu ◽  
Ying Wang ◽  
Chengxin Zhang ◽  
Ying Lin ◽  
Feng Li
Keyword(s):  
Signal Amplification ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Atp Assay

scholarly journals Highly Sensitive and Selective Copper (II)-Catalyzed Dual-DNAzyme Colorimetric Biosensor Based on Exonuclease III-Mediated Cyclical Assembly

Catalysts ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1352
Author(s):  
Baiqiang Zhai ◽  
Kunlun Huang ◽  
Hongtao Wang ◽  
Dongmin Su ◽  
Yuancong Xu
Keyword(s):  
Metal Ion ◽  
Limit Of Detection ◽  
Rapid Determination ◽  
High Sensitivity ◽  
General Purpose ◽  
Exonuclease Iii ◽  
Dna Cleaving ◽  
Transition Sequence ◽  
Highly Sensitive ◽  
Exo Iii

“Cu-DNAzyme” and “G4-DNAzyme” were used to develop a “turn-off” dual-DNAzyme colorimetric biosensor, which could be used to detect Cu2+ by employing exonuclease III-mediated cyclical assembly (EMCA). EMCA was based on the cleavage activity of Cu2+ to transfer the linkage sequences of the substrate strand and enzyme strand into the transition sequence. The horseradish peroxidase (HRP)-mimicking activity of the G4-DNAzyme was lost after binding with the complementary transition sequence and was hydrolyzed by Exo III. These results demonstrate that the proposed colorimetric biosensor was an effective method for ultradetection of trace metals in a high original signal background. Due to the high sensitivity of the biosensor, the limit of detection (LOD) of Cu2+ is 0.16 nM. This design offers a general purpose platform that could be applied for the detection of any metal ion target through adjustment of metal-dependent DNA-cleaving DNAzymes, which is of great significance for the rapid determination of food safety.

A new mode for highly sensitive and specific detection of DNA based on exonuclease III-assisted target recycling amplification and mismatched catalytic hairpin assembly

2015 ◽  
Vol 51 (20) ◽  
pp. 4220-4222 ◽  
Author(s):  
Chenyang Tao ◽  
Yurong Yan ◽  
Hua Xiang ◽  
Dan Zhu ◽  
Wei Cheng ◽  
...  
Keyword(s):  
Dna Detection ◽  
Specific Detection ◽  
Schematic Representation ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Target Dna ◽  
Highly Sensitive ◽  
Catalytic Hairpin Assembly ◽  
Recycling Amplification

Schematic representation of the designed strategy for target DNA detection.

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