Synthetic sialylglycopolymer receptor for virus detection using cantilever-based sensors

The Analyst ◽  
2015 ◽  
Vol 140 (17) ◽  
pp. 6131-6137 ◽  
Author(s):  
P. V. Gorelkin ◽  
A. S. Erofeev ◽  
G. A. Kiselev ◽  
D. V. Kolesov ◽  
E. V. Dubrovin ◽  
...  
Keyword(s):  
Influenza A ◽  
Virus Detection ◽  
Label Free ◽  
Influenza A Viruses ◽  
Label Free Detection ◽  
Receptor Layer

We describe the rapid, label-free detection of Influenza A viruses using a cantilever transducer modified with a synthetic sialylglycopolymer receptor layer.

scholarly journals Label-free sensitive detection of influenza virus using PZT discs with a synthetic sialylglycopolymer receptor layer

2019 ◽  
Vol 6 (9) ◽  
pp. 190255 ◽  
Author(s):  
Alexander S. Erofeev ◽  
Petr V. Gorelkin ◽  
Dmitry V. Kolesov ◽  
Gleb A. Kiselev ◽  
Evgeniy V. Dubrovin ◽  
...  
Keyword(s):  
Lead Zirconate Titanate ◽  
Influenza A ◽  
High Sensitivity ◽  
Influenza Viruses ◽  
Radial Mode ◽  
Sensitive Detection ◽  
Label Free ◽  
Influenza A Viruses ◽  
Label Free Detection ◽  
Receptor Layer

We describe rapid, label-free detection of Influenza A viruses using the first radial mode of oscillations of lead zirconate titanate (PZT) piezoelectric discs with a 2 mm radius and 100 µm thickness fabricated from a piezoelectric membrane. The discs are modified with a synthetic sialylglycopolymer receptor layer, and the coated discs are inserted in a flowing virus suspension. Label-free detection of the virus is achieved by monitoring the disc radial mode resonance frequency shift. Piezo transducers with sialylglycopolymer sensor layers exhibited a long lifetime, a high sensitivity and the possibility of regeneration. We demonstrate positive, label-free detection of Influenza A viruses at concentrations below 10 5 virus particles per millilitre. We show that label-free, selective, sensitive detection of influenza viruses by home appliances is possible in principle.

scholarly journals Field-Effect Sensors for Virus Detection: From Ebola to SARS-CoV-2 and Plant Viral Enhancers

2020 ◽  
Vol 11 ◽  
Author(s):  
Arshak Poghossian ◽  
Melanie Jablonski ◽  
Denise Molinnus ◽  
Christina Wege ◽  
Michael J. Schöning
Keyword(s):  
Field Effect ◽  
Point Of Care ◽  
Virus Detection ◽  
Fast Response ◽  
Plant Viruses ◽  
Clinical Samples ◽  
Label Free ◽  
Label Free Detection ◽  
Field Effect Devices ◽  
Long Time

Coronavirus disease 2019 (COVID-19) is a novel human infectious disease provoked by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Currently, no specific vaccines or drugs against COVID-19 are available. Therefore, early diagnosis and treatment are essential in order to slow the virus spread and to contain the disease outbreak. Hence, new diagnostic tests and devices for virus detection in clinical samples that are faster, more accurate and reliable, easier and cost-efficient than existing ones are needed. Due to the small sizes, fast response time, label-free operation without the need for expensive and time-consuming labeling steps, the possibility of real-time and multiplexed measurements, robustness and portability (point-of-care and on-site testing), biosensors based on semiconductor field-effect devices (FEDs) are one of the most attractive platforms for an electrical detection of charged biomolecules and bioparticles by their intrinsic charge. In this review, recent advances and key developments in the field of label-free detection of viruses (including plant viruses) with various types of FEDs are presented. In recent years, however, certain plant viruses have also attracted additional interest for biosensor layouts: Their repetitive protein subunits arranged at nanometric spacing can be employed for coupling functional molecules. If used as adapters on sensor chip surfaces, they allow an efficient immobilization of analyte-specific recognition and detector elements such as antibodies and enzymes at highest surface densities. The display on plant viral bionanoparticles may also lead to long-time stabilization of sensor molecules upon repeated uses and has the potential to increase sensor performance substantially, compared to conventional layouts. This has been demonstrated in different proof-of-concept biosensor devices. Therefore, richly available plant viral particles, non-pathogenic for animals or humans, might gain novel importance if applied in receptor layers of FEDs. These perspectives are explained and discussed with regard to future detection strategies for COVID-19 and related viral diseases.

Direct label-free detection of Rotavirus using a hydrogel based nanoporous photonic crystal

RSC Advances ◽  
2016 ◽  
Vol 6 (9) ◽  
pp. 7384-7390 ◽  
Author(s):  
Bohee Maeng ◽  
Youngkyu Park ◽  
Jungyul Park
Keyword(s):  
Photonic Crystal ◽  
Crystal Structures ◽  
Virus Detection ◽  
Label Free ◽  
Label Free Detection ◽  
Near Future ◽  
Direct Label

A direct label-free biosensor based on 3D photonic crystal structures for Rotavirus has been demonstrated. This proposed method will be useful for developing a direct and easy-to-use virus detection kit in the form of POCT in the near future.

scholarly journals Characterization of Receptor Binding Profiles of Influenza A Viruses Using An Ellipsometry-Based Label-Free Glycan Microarray Assay Platform

Biomolecules ◽  
2015 ◽  
Vol 5 (3) ◽  
pp. 1480-1498 ◽  
Author(s):  
Yiyan Fei ◽  
Yung-Shin Sun ◽  
Yanhong Li ◽  
Hai Yu ◽  
Kam Lau ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Influenza A ◽  
Label Free ◽  
Influenza A Viruses ◽  
Microarray Assay ◽  
Glycan Microarray

scholarly journals Biomimetic Nanopillar-Based Biosensor for Label-Free Detection of Influenza A Virus

2021 ◽  
Author(s):  
Wang Sik Lee ◽  
Junhyoung Ahn ◽  
Sanghee Jung ◽  
Jaejong Lee ◽  
Taejoon Kang ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Label Free ◽  
Label Free Detection

scholarly journals A rapid and label-free platform for virus capture and identification from clinical samples

2019 ◽  
Vol 117 (2) ◽  
pp. 895-901 ◽  
Author(s):  
Yin-Ting Yeh ◽  
Kristen Gulino ◽  
YuHe Zhang ◽  
Aswathy Sabestien ◽  
Tsui-Wen Chou ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Real Time ◽  
Influenza A ◽  
Respiratory Infections ◽  
Surface Enhanced Raman Spectroscopy ◽  
Clinical Samples ◽  
Label Free ◽  
Influenza A Viruses ◽  
Virus Identification ◽  
Surface Enhanced Raman

Emerging and reemerging viruses are responsible for a number of recent epidemic outbreaks. A crucial step in predicting and controlling outbreaks is the timely and accurate characterization of emerging virus strains. We present a portable microfluidic platform containing carbon nanotube arrays with differential filtration porosity for the rapid enrichment and optical identification of viruses. Different emerging strains (or unknown viruses) can be enriched and identified in real time through a multivirus capture component in conjunction with surface-enhanced Raman spectroscopy. More importantly, after viral capture and detection on a chip, viruses remain viable and get purified in a microdevice that permits subsequent in-depth characterizations by various conventional methods. We validated this platform using different subtypes of avian influenza A viruses and human samples with respiratory infections. This technology successfully enriched rhinovirus, influenza virus, and parainfluenza viruses, and maintained the stoichiometric viral proportions when the samples contained more than one type of virus, thus emulating coinfection. Viral capture and detection took only a few minutes with a 70-fold enrichment enhancement; detection could be achieved with as little as 102 EID50/mL (50% egg infective dose per microliter), with a virus specificity of 90%. After enrichment using the device, we demonstrated by sequencing that the abundance of viral-specific reads significantly increased from 4.1 to 31.8% for parainfluenza and from 0.08 to 0.44% for influenza virus. This enrichment method coupled to Raman virus identification constitutes an innovative system that could be used to quickly track and monitor viral outbreaks in real time.

Sepsis in Infants

1991 ◽  
Vol 12 (11) ◽  
pp. 330-351
Keyword(s):  
Influenza A ◽  
Bacterial Infections ◽  
Viral Infections ◽  
Seasonal Pattern ◽  
Virus Detection ◽  
Patient Treatment ◽  
Causative Organism ◽  
Influenza A Viruses ◽  
Suspected Sepsis ◽  
A Prospective Study

A prospective study was conducted to determine the frequency and distribution of bacterial and viral pathogens in infants hospitalized with suspected sepsis and to evaluate the potential of virus detection for improving patient treatment. A causative organism was detected in 157 (67%) of 233 previously healthy infants aged less than 3 months who had been hospitalized for suspected sepsis: 19 (8%) had bacterial infections, 135 (58%) had viral infections, and 3 (1%) had mixed viral-bacterial infections. Viral infections occurred in a seasonal pattern: enteroviruses were responsible for most of the hospitalizations during summer and fall (65 of 110, 63%), and respiratory syncytial and influenza A viruses were responsible for most of the infections during winter (44 of 81, 55%).

TMPRSS2 is essential for pathogenicity of H7N9 and H1N1 influenza A viruses in mice

Pneumologie ◽  
2014 ◽  
Vol 68 (02) ◽  
Author(s):  
C Tarnow ◽  
G Engels ◽  
A Arendt ◽  
F Schwalm ◽  
H Sediri ◽  
...  
Keyword(s):  
Influenza A ◽  
H1n1 Influenza ◽  
Influenza A Viruses

Killing two birds with one stone – Prenylated flavonoids disrupt the lethal synergism of influenza A viruses and pneumococci

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
U Grienke ◽  
M Richter ◽  
E Walther ◽  
A Hoffmann ◽  
J Kirchmair ◽  
...  
Keyword(s):  
Influenza A ◽  
Influenza A Viruses ◽  
Prenylated Flavonoids
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