Direct label-free detection of Rotavirus using a hydrogel based nanoporous photonic crystal

RSC Advances ◽  
2016 ◽  
Vol 6 (9) ◽  
pp. 7384-7390 ◽  
Author(s):  
Bohee Maeng ◽  
Youngkyu Park ◽  
Jungyul Park
Keyword(s):  
Photonic Crystal ◽  
Crystal Structures ◽  
Virus Detection ◽  
Label Free ◽  
Label Free Detection ◽  
Near Future ◽  
Direct Label

A direct label-free biosensor based on 3D photonic crystal structures for Rotavirus has been demonstrated. This proposed method will be useful for developing a direct and easy-to-use virus detection kit in the form of POCT in the near future.

Photonic crystals: A platform for label-free and enhanced fluorescence biomolecular and cellular assays

2008 ◽  
Vol 1133 ◽  
Author(s):  
Brian T. Cunningham ◽  
Leo Chan ◽  
Patrick C. Mathias ◽  
Nikhil Ganesh ◽  
Sherine George ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Photonic Crystals ◽  
High Throughput Screening ◽  
High Sensitivity ◽  
Excitation Wavelength ◽  
Label Free ◽  
Crystal Surfaces ◽  
Enhanced Fluorescence ◽  
Preferred Direction ◽  
Label Free Detection

Abstract Photonic crystal surfaces represent a class of resonant optical structures that are capable of supporting high intensity electromagnetic standing waves with near-field and far-field properties that can be exploited for high sensitivity detection of biomolecules and cells. While modulation of the resonant wavelength of a photonic crystal by the dielectric permittivity of adsorbed biomaterials enables label-free detection, the resonance can also be tuned to coincide with the excitation wavelength of common fluorescent tags - including organic molecules and semiconductor quantum dots. Photonic crystals are also capable of efficiently channeling fluorescent emission into a preferred direction for enhanced extraction efficiency. Photonic crystals can be designed to support multiple resonant modes that can perform label free detection, enhanced fluorescence excitation, and enhanced fluorescence extraction simultaneously on the same device. Because photonic crystal surfaces may be inexpensively produced over large surface areas by nanoreplica molding processes, they can be incorporated into disposable labware for applications such as pharmaceutical high throughput screening. In this talk, the optical properties of surface photonic crystals will be reviewed and several applications will be described, including results from screening a 200,000-member chemical compound library for inhibitors of protein-DNA interactions, gene expression microarrays, and high sensitivity of protein biomarkers.

scholarly journals Field-Effect Sensors for Virus Detection: From Ebola to SARS-CoV-2 and Plant Viral Enhancers

2020 ◽  
Vol 11 ◽  
Author(s):  
Arshak Poghossian ◽  
Melanie Jablonski ◽  
Denise Molinnus ◽  
Christina Wege ◽  
Michael J. Schöning
Keyword(s):  
Field Effect ◽  
Point Of Care ◽  
Virus Detection ◽  
Fast Response ◽  
Plant Viruses ◽  
Clinical Samples ◽  
Label Free ◽  
Label Free Detection ◽  
Field Effect Devices ◽  
Long Time

Coronavirus disease 2019 (COVID-19) is a novel human infectious disease provoked by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Currently, no specific vaccines or drugs against COVID-19 are available. Therefore, early diagnosis and treatment are essential in order to slow the virus spread and to contain the disease outbreak. Hence, new diagnostic tests and devices for virus detection in clinical samples that are faster, more accurate and reliable, easier and cost-efficient than existing ones are needed. Due to the small sizes, fast response time, label-free operation without the need for expensive and time-consuming labeling steps, the possibility of real-time and multiplexed measurements, robustness and portability (point-of-care and on-site testing), biosensors based on semiconductor field-effect devices (FEDs) are one of the most attractive platforms for an electrical detection of charged biomolecules and bioparticles by their intrinsic charge. In this review, recent advances and key developments in the field of label-free detection of viruses (including plant viruses) with various types of FEDs are presented. In recent years, however, certain plant viruses have also attracted additional interest for biosensor layouts: Their repetitive protein subunits arranged at nanometric spacing can be employed for coupling functional molecules. If used as adapters on sensor chip surfaces, they allow an efficient immobilization of analyte-specific recognition and detector elements such as antibodies and enzymes at highest surface densities. The display on plant viral bionanoparticles may also lead to long-time stabilization of sensor molecules upon repeated uses and has the potential to increase sensor performance substantially, compared to conventional layouts. This has been demonstrated in different proof-of-concept biosensor devices. Therefore, richly available plant viral particles, non-pathogenic for animals or humans, might gain novel importance if applied in receptor layers of FEDs. These perspectives are explained and discussed with regard to future detection strategies for COVID-19 and related viral diseases.

LSPR-enhanced photonic crystal allows ultrasensitive and label-free detection of hazardous chemicals

2020 ◽  
Vol 310 ◽  
pp. 127671 ◽  
Author(s):  
Yanqiu Song ◽  
Jialei Bai ◽  
Rong Zhang ◽  
Enqi Wu ◽  
Jiang Wang ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Label Free ◽  
Hazardous Chemicals ◽  
Label Free Detection

Double-layer Fano resonance photonic-crystal-slab-based sensor for label-free detection of different size analytes

2019 ◽  
Vol 36 (2) ◽  
pp. 215 ◽  
Author(s):  
Zheng Wang ◽  
Chao Wang ◽  
Fujun Sun ◽  
Zhongyuan Fu ◽  
Zekun Xiao ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Double Layer ◽  
Fano Resonance ◽  
Label Free ◽  
Photonic Crystal Slab ◽  
Label Free Detection

scholarly journals Label-free detection of cardiac troponin I with a photonic crystal biosensor

2014 ◽  
Vol 58 ◽  
pp. 107-113 ◽  
Author(s):  
Bailin Zhang ◽  
Andres W. Morales ◽  
Ralph Peterson ◽  
Liang Tang ◽  
Jing Yong Ye
Keyword(s):  
Photonic Crystal ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Cardiac Troponin I ◽  
Label Free ◽  
Label Free Detection
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