A sensitive biosensor with a DNAzyme for lead(ii) detection based on fluorescence turn-on

The Analyst ◽  
2015 ◽  
Vol 140 (13) ◽  
pp. 4642-4647 ◽  
Author(s):  
Yang Guo ◽  
Junting Li ◽  
Xiaoqian Zhang ◽  
Yanli Tang
Keyword(s):  
High Sensitivity ◽  
Optical Biosensor ◽  
Lead Ions ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
Fluorescence Turn On

A new optical biosensor for detection of lead ions has been constructed based on fluorescence turn-on, which demonstrates high sensitivity and selectivity without any additional procedures.

Improved Detection of Hg2+ in Aqueous Solution with High Sensitivity and Selectivity by Using Mercury-Specific DNA, Sybr Green I and Gold Nanoparticles

2011 ◽  
Vol 239-242 ◽  
pp. 934-939
Author(s):  
Hui Xu ◽  
Shuli Gao ◽  
Jian Nong Chen ◽  
Quan Wen Liu
Keyword(s):  
Gold Nanoparticles ◽  
High Sensitivity ◽  
Sybr Green ◽  
Sybr Green I ◽  
Fluorescence Signal ◽  
Potassium Ions ◽  
Label Free ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
Fluorescence Turn On

We report a label-free, fast, fluorescence turn on assay for Hg2+detecton by using mercury-specific DNA (MSD), Sybr Green I (SG) and gold nanoparticles (AuNPs). SG efficiently discriminates MSD and MSD/Hg2+complex. The addition of gold nanoparticle decreases the background fluorescence signal further for MSD. The fluorescence intensity of MSD/Hg2+complex keeps constant after addition of AuNPs. This property improves the signal-to-background ratio and decreases the detection limitation further. In addition, the method shows improved selectivity compared with that in the absence of AuNPs. This strategy could be applied to the detection of potassium ions and showed good generality.

Amplified Fluorescence Turn-on Assay for Mercury(II) Based on Conjugated Polyfluorene Derivatives and Nanospheres

2008 ◽  
Vol 1134 ◽  
Author(s):  
Yusong Wang ◽  
Bin Liu
Keyword(s):  
Metal Ion ◽  
Resonance Energy Transfer ◽  
Fluorescence Emission ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Probe Design ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
Fluorescence Turn On ◽  
Polyfluorene Derivatives

AbstractDetection of mercury with high sensitivity and selectivity constitutes a significant research concern. Here, we report an amplified fluorescence turn-on assay for mercury(II) with an improved performance. This sensing system takes advantage of optically amplifying fluorescent conjugated polyfluorene derivatives and DNA immobilized silica nanospheres (NSs) in addition to the specific thymine- mercury(II)-thymine(T- Hg2+-T) interaction. The employment of ion-specific T- Hg2+-T coordination increases the melting temperature (Tm) of the double-stranded DNA (dsDNA) on the hybridized NS surface. After thermal washing at 45 °C, the Hg2+ treated sample (dsDNA-NS) was effectively differentiated from that treated with nonspecific ions through monitoring fluorescence emission of fluorescein (Fl) labeled target DNA remained on the NS surface. Finally, a cationic conjugated polyfluorene derivative (CCP) was introduced to electrostatically associate with the DNA molecules on the NS surface, resulting in an amplified Fl signal via fluorescence resonance energy transfer (FRET) from the CCP to the dye molecule. In comparison with the use of Fl alone as a signal reporter, the presence of CCP significantly enhances the detection fluorescence intensity, reduces false-positive signal, and improves the detection selectivity for mercury(II). Further improvement in the probe design could yield more efficient metal ion sensors, which have the potential to be operated at room temperature and for the detection of other metal ions besides mercury(II).

A turn-on luminescent probe for Fe3+ and ascorbic acid with logic gate operation based on a zinc(ii)-based metal–organic framework

2020 ◽  
Vol 44 (21) ◽  
pp. 8728-8735 ◽  
Author(s):  
Jing Tang ◽  
Doudou Feng ◽  
Jie Yang ◽  
Xuehui Ma ◽  
Xiao-Qing Wang
Keyword(s):  
Metal Organic Framework ◽  
Logic Gate ◽  
High Sensitivity ◽  
Luminescent Probe ◽  
Gate Operation ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
Metal Organic ◽  
Fluorescence Turn On ◽  
Organic Framework

A zinc(ii)-based metal–organic framework exhibits fluorescence turn-on behaviour for Fe3+ and AA with high sensitivity and selectivity.

A highly sensitive turn-on ratiometric luminescent probe based on postsynthetic modification of Tb3+@Cu-MOF for H2S detection

2017 ◽  
Vol 5 (38) ◽  
pp. 9943-9951 ◽  
Author(s):  
Xubin Zheng ◽  
Ruiqing Fan ◽  
Yang Song ◽  
Ani Wang ◽  
Kai Xing ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Luminescent Probe ◽  
Turn On ◽  
Postsynthetic Modification ◽  
Highly Sensitive ◽  
Sensitivity And Selectivity ◽  
H2s Detection

A novel probe, designed and prepared through PSM of Cu-MOFs and Tb3+ions, shows high sensitivity and selectivity for H2S.

A highly sensitive and selective fluorescent turn-on chemosensor bearing a 7-diethylaminocoumarin moiety for the detection of cyanide in organic and aqueous solutions

2020 ◽  
Vol 44 (44) ◽  
pp. 19155-19165
Author(s):  
Burcu Aydıner ◽  
Ömer Şahin ◽  
Deniz Çakmaz ◽  
Gökhan Kaplan ◽  
Kerem Kaya ◽  
...  
Keyword(s):  
Aqueous Solutions ◽  
Emission Intensity ◽  
High Sensitivity ◽  
Turn On ◽  
Highly Sensitive ◽  
Sensitivity And Selectivity

Novel 7-diethylaminocoumarin based dyes showed high sensitivity and selectivity to cyanide anions in both organic and aqueous solutions, which was observed by a drastic increment in the emission intensity.

A dual-emitting Tb(iii)&Yb(iii)-functionalized coordination polymer: a “turn-on” sensor for N-methylformamide in urine and a “turn-off” sensor for methylglyoxal in serum

2019 ◽  
Vol 48 (38) ◽  
pp. 14408-14417 ◽  
Author(s):  
Xubin Zheng ◽  
Ruiqing Fan ◽  
Haoyang Lu ◽  
Bowen Wang ◽  
Jingkun Wu ◽  
...  
Keyword(s):  
Coordination Polymer ◽  
Fluorescent Probe ◽  
High Sensitivity ◽  
Turn On ◽  
Postsynthetic Modification ◽  
Sensitivity And Selectivity

A fluorescent probe designed and prepared through postsynthetic modification (PSM) showed high sensitivity and selectivity for N-methylformamide (NMF) in urine and methylglyoxal (MGO) in serum.

Pyrene appended thymine derivative for selective turn-on fluorescence sensing of uric acid in live cells

RSC Advances ◽  
2016 ◽  
Vol 6 (71) ◽  
pp. 66774-66778 ◽  
Author(s):  
Prithidipa Sahoo ◽  
Himadri Sekhar Sarkar ◽  
Sujoy Das ◽  
Kalipada Maiti ◽  
Md Raihan Uddin ◽  
...  
Keyword(s):  
Uric Acid ◽  
Fluorescent Probe ◽  
High Sensitivity ◽  
Living Cells ◽  
Live Cells ◽  
Fluorescence Sensing ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
First Time

A new ‘turn-ON’ fluorescent probe, pyrene appended thymine acetamide (PTA), with high sensitivity and selectivity for the detection of uric acid (UA) was developed and first time imaging of uric acid in living cells in water was achieved.

Nitrogen doped carbon dots: mechanism investigation and their application for label free CA125 analysis

2019 ◽  
Vol 7 (19) ◽  
pp. 3053-3058 ◽  
Author(s):  
Yuanyuan Liu ◽  
Liping Jiang ◽  
Bijun Li ◽  
Xinyue Fan ◽  
Wei Wang ◽  
...  
Keyword(s):  
Nitrogen Doping ◽  
Competitive Adsorption ◽  
High Sensitivity ◽  
Label Free ◽  
Nitrogen Doped ◽  
Microwave Assisted ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

Nitrogen-doped CDs (N-CDs) were firstly prepared by using pear juice as the carbon source and ethanediamine as a nitrogen doping precursor with a microwave assisted pyrolysis technique. Based on the fluorescence recovery induced by competitive adsorption and desorption, a label-free “turn on” fluorescence assay with high sensitivity and selectivity was proposed for the analysis of CA125.

scholarly journals Chiral CdSe nanoplatelets as an ultrasensitive probe for lead ion sensing

Nanoscale ◽  
2019 ◽  
Vol 11 (19) ◽  
pp. 9327-9334 ◽  
Author(s):  
Xiongbin Wang ◽  
Junjie Hao ◽  
Jiaji Cheng ◽  
Junzi Li ◽  
Jun Miao ◽  
...  
Keyword(s):  
Ligand Exchange ◽  
High Sensitivity ◽  
Lead Ions ◽  
Lead Ion ◽  
Ion Sensing ◽  
Sensitivity And Selectivity

Achiral CdSe NPLs could show chirality through ligand exchange with chiral cysteine molecules. Chiral CdSe NPLs were successfully applied as a chiral probe to detect lead ions with high sensitivity and selectivity.

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