Imaging phosphorylated peptide distribution in human lens by MALDI MS

The Analyst ◽  
2015 ◽  
Vol 140 (12) ◽  
pp. 4284-4290 ◽  
Author(s):  
Jing Jiao ◽  
Aizhu Miao ◽  
Ying Zhang ◽  
Qi Fan ◽  
Yi Lu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Maldi Ms ◽  
Human Lens ◽  
New Strategy ◽  
Phosphorylated Peptide

A new strategy for on-tissue dephosphorylation treatment was established for phosphorylated peptide distribution by mass spectrometry imaging.

scholarly journals Distribution of Glycerophospholipids in the Adult Human Lens

Biomolecules ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 156 ◽  
Author(s):  
Jo Seng ◽  
Jessica Nealon ◽  
Stephen Blanksby ◽  
Todd Mitchell
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Imaging Mass Spectrometry ◽  
Human Lens ◽  
Limited Data ◽  
Outer Cortex ◽  
Adult Human ◽  
Cell Age ◽  
Human Lenses ◽  
The Relationship

In humans, the age of fibre cells differs across the ocular lens, ranging from those formed before birth in the core of the lens to those formed just prior to death in the outer cortex. The distribution of glycerophospholipids in the adult human lens should reflect this range; however, limited data currently exists to confirm this hypothesis. Accordingly, this study aimed to determine the distribution of glycerophospholipids in adult human lens using mass spectrometry imaging. To achieve this, 20-µm thick slices of two human lenses, aged 51 and 67 were analysed by matrix-assisted laser desorption ionisation imaging mass spectrometry. The data clearly indicate that intact glycerophospholipids such as phosphatidylethanolamine, phosphatidylserine, and phosphatidic acid are mainly present in the outer cortex region, corresponding to the youngest fibre cells, while lyso-phosphatidylethanolamine, likely produced by the degradation of phosphatidylethanolamine, is present in the nucleus (older fibre cells). This study adds further evidence to the relationship between fibre cell age and glycerophospholipid composition.

Age-Related Changes in the Lateral Lipid Distribution in a Human Lens Described by Mass Spectrometry Imaging

2015 ◽  
Vol 21 (3) ◽  
pp. 297-303 ◽  
Author(s):  
Jaroslav Pol ◽  
Helena Faltyskova ◽  
Lukas Krasny ◽  
Michael Volný ◽  
Ondrej Vlacil ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Human Lens ◽  
Lipid Distribution ◽  
Age Related ◽  
Age Related Changes

scholarly journals Study of oxaliplatin penetration into ovaries of patients treated with hyperthermic intraperitoneal chemotherapy (HIPEC) for peritoneal metastases of colorectal and appendiceal origin using mass spectrometry imaging

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Marion Larroque ◽  
Sandra Mounicou ◽  
Olivia Sgarbura ◽  
Carine Arnaudguilhem ◽  
Lucie Rebel ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Intraperitoneal Chemotherapy ◽  
Mass Spectrometry Imaging ◽  
Hyperthermic Intraperitoneal Chemotherapy ◽  
Imaging Techniques ◽  
Peritoneal Metastases ◽  
Maldi Ms ◽  
Ionization Mass ◽  
Childbearing Age ◽  
Icp Ms

Abstract Objectives Platinum salts are commonly used in hyperthermic intraperitoneal chemotherapy (HIPEC) for digestive tract cancer treatment. During HIPEC with oxaliplatin for peritoneal metastases (PMs) treatment, the ovaries are directly exposed to the drug, questioning about ovarian resection and the potential impact of the drug on ovarian functionality, especially in young women of childbearing age. The goal of this work is to understand unwanted damages to the ovaries during HIPEC therapy by the determination of the concentration and distribution of platinum in ovaries in order to address its potential toxicity. Methods Mass spectrometry imaging techniques, matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP MS), were used to study the penetration of oxaliplatin in ovaries after HIPEC treatment. Results MALDI-MS allowed the localization of an oxaliplatin-derivative (m/z 456.2) at the periphery of the ovaries. The quantitative LA-ICP MS maps confirmed the localization of elemental platinum as well as in the central part of ovaries from patients who received a previous platinum salt-based chemotherapy. Conclusions LA-ICP MS images showed that platinum diffusion was extended in cases of previous systemic treatment, questioning about platinum derivatives gonado-toxicity when combining the two treatments.

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