Synthesis, characterization and evaluation of collagen scaffolds crosslinked with aminosilane functionalized silver nanoparticles: in vitro and in vivo studies

2015 ◽  
Vol 3 (15) ◽  
pp. 3032-3043 ◽  
Author(s):  
Abhishek Mandal ◽  
Santhanam Sekar ◽  
N. Chandrasekaran ◽  
Amitava Mukherjee ◽  
Thotapalli P. Sastry
Keyword(s):  
Silver Nanoparticles ◽  
Clinical Applications ◽  
In Vivo Studies ◽  
Cross Linking ◽  
Collagen Scaffolds ◽  
Novel Approach

This work presents a novel approach for functionalization of silver nanoparticles and cross-linking them with collagen to form FSCSC scaffolds suitable for clinical applications.

scholarly journals Preparation and preclinical evaluation of aceclofenac loaded pectinate mucoadhesive microspheres

2012 ◽  
Vol 2 (1) ◽  
pp. 8 ◽  
Author(s):  
Santanu Chakraborty ◽  
Priyanka Nayak ◽  
Bala Murali Krishna ◽  
Madhusmruti Khandai ◽  
Ashoke Kumar Ghosh
Keyword(s):  
Particle Size ◽  
Drug Release ◽  
Polymer Concentration ◽  
Research Work ◽  
In Vivo Studies ◽  
Systemic Absorption ◽  
Cross Linking ◽  
Significant Difference

The aim of the present research work was to fabricate aceclofenac loaded pectinate microspheres by ionic gelation method and evaluate the effect of different cross-linking agents and polymer concentration on particle size, encapsulation efficacy and drug release behavior. It was also investigated that whether this pectinate dosage form was able to target the drug release in intestinal region and prevent the different side effect associated with the drug in stomach or not. It was observed that particle size, encapsulation efficacy and in vitro drug release were largely depended on polymer concentration and cross-linking agents. It was also observed that pectinate microspheres showed excellent pH depended mucoadhesive properties and they were able to restrict the drug release in stomach. <em>In vitro</em> drug release study showed that alminium-pectinate microspheres have more sustaining property as compared to barium-pectinate microspheres. Holm-Sidak multiple comparison analysis suggested a significant difference in measured t<sub>50%</sub> values among all the formulations with same cross-linking agent. In vivo studies revealed that the anti inflammatory and analgesic effects induced by pectinate microspheres were significantly high and prolonged as compared to pure drug. So, pectinate microspheres can be an excellent carrier for targeting the delivery of aceclofenac as well as help in improving the patient compliance by prolonging the systemic absorption.

scholarly journals Cross-linking Electrospun Polydioxanone-Soluble Elastin Blends: Material Characterization

2008 ◽  
Vol 3 (1) ◽  
pp. 155892500800300 ◽  
Author(s):  
Michael J. McClure ◽  
Scott A. Sell ◽  
Catherine P. Barnes ◽  
Whitney C. Bowen ◽  
Gary L. Bowlin
Keyword(s):  
Femoral Artery ◽  
Material Properties ◽  
In Vivo Studies ◽  
Uniaxial Tensile ◽  
Cross Linking ◽  
Materials Testing ◽  
Uniaxial Tensile Testing ◽  
The Cross

The purpose of this study was to establish whether material properties of elastin co-electrospun with polydioxanone (PDO) would change over time in both the uncross-linked state and the cross-linked state. First, uncross-linked scaffolds were placed in phosphate buffered saline (PBS) for three separate time periods: 15 minutes, 1 hour, and 24 hours, and subsequently tested using uniaxial materials testing. Several cross-linking reagents were then investigated to verify their ability to crosslink elastin: 1–ethyl-3–(dimethylaminopropyl)-carbodiimide (EDC), ethylene glycol diglycidyl ether (EGDE), and genipin. Uniaxial tensile testing was performed on scaffolds cross-linked with EDC and genipin, yielding results that warranted further investigation for PDO-elastin blends. Material properties of the cross-linked scaffolds were then found within range of both pig femoral artery and human femoral artery. These results demonstrate PDO-elastin blends could potentially be favorable as vascular grafts, thus warranting future in vitro and in vivo studies.

scholarly journals Aloe vera: An Ancient Herb for Modern Dentistry—A Literature Review

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Arbaz Sajjad ◽  
Samia Subhani Sajjad
Keyword(s):  
Aloe Vera ◽  
Clinical Applications ◽  
In Vivo Studies ◽  
Cochrane Central Register ◽  
Patient Centered ◽  
Aphthous Ulcers ◽  
Central Register ◽  
Dental Diseases

Objectives. To review composition, actions, and clinical applications of Aloe vera plant in dentistry and to establish its effectiveness as an invaluable adjunct in the treatment of dental diseases. Method. A manual and electronic literature (MEDLINE, Cochrane Central Register of Controlled Trials, and Google Scholar) search was performed up to July 2013 for in vitro and in vivo studies and research presenting clinical, microbiological, immunological, and patient-centered data to validate the efficacy of Aloe vera gel in dentistry. A total of 38 titles, abstracts, and full-text studies were selected and reviewed. Aloe vera has various medicinal properties like anti-inflammatory, antibacterial, antiviral, and antitumor which accelerates wound healing and helps in treating various lesions in oral cavity. Benefits associated with Aloe vera have been attributed to the polysaccharides contained in the gel of the leaves. Conclusion. The pharmacological attributes of Aloe vera have been revalidated in modern sciences through various in vivo and in vitro studies. The herb has immense potential as a dental therapeutic. Even though Aloe vera is a promising herb with various clinical applications in medicine and dentistry, more clinical research needs to be undertaken especially to validate and explain the action of acemannan hydrogel in accelerating the healing of aphthous ulcers and to validate the efficacy of Aloe gel on plaque and gingivitis, so that it can be established in the field of dentistry.

scholarly journals Anti-Inflammatory Fibronectin-AgNP for Regulation of Biological Performance and Endothelial Differentiation Ability of Mesenchymal Stem Cells

2021 ◽  
Vol 22 (17) ◽  
pp. 9262
Author(s):  
Huey-Shan Hung ◽  
Kai-Bo Chang ◽  
Cheng-Ming Tang ◽  
Tian-Ren Ku ◽  
Mei-Lang Kung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Immune Response ◽  
Silver Nanoparticles ◽  
Mesenchymal Stem Cells ◽  
Superior Performance ◽  
In Vivo Studies ◽  
Vascular Regeneration ◽  
Anti Inflammatory

The engineering of vascular regeneration still involves barriers that need to be conquered. In the current study, a novel nanocomposite comprising of fibronectin (denoted as FN) and a small amount of silver nanoparticles (AgNP, ~15.1, ~30.2 or ~75.5 ppm) was developed and its biological function and biocompatibility in Wharton’s jelly-derived mesenchymal stem cells (MSCs) and rat models was investigated. The surface morphology as well as chemical composition for pure FN and the FN-AgNP nanocomposites incorporating various amounts of AgNP were firstly characterized by atomic force microscopy (AFM), UV-Visible spectroscopy (UV-Vis), and Fourier-transform infrared spectroscopy (FTIR). Among the nanocomposites, FN-AgNP with 30.2 ppm silver nanoparticles demonstrated the best biocompatibility as assessed through intracellular ROS production, proliferation of MSCs, and monocytes activation. The expression levels of pro-inflammatory cytokines, TNF-α, IL-1β, and IL-6, were also examined. FN-AgNP 30.2 ppm significantly inhibited pro-inflammatory cytokine expression compared to other materials, indicating superior performance of anti-immune response. Mechanistically, FN-AgNP 30.2 ppm significantly induced greater expression of vascular endothelial growth factor (VEGF) and stromal-cell derived factor-1 alpha (SDF-1α) and promoted the migration of MSCs through matrix metalloproteinase (MMP) signaling pathway. Besides, in vitro and in vivo studies indicated that FN-AgNP 30.2 ppm stimulated greater protein expressions of CD31 and von Willebrand Factor (vWF) as well as facilitated better endothelialization capacity than other materials. Furthermore, the histological tissue examination revealed the lowest capsule formation and collagen deposition in rat subcutaneous implantation of FN-AgNP 30.2 ppm. In conclusion, FN-AgNP nanocomposites may facilitate the migration and proliferation of MSCs, induce endothelial cell differentiation, and attenuate immune response. These finding also suggests that FN-AgNP may be a potential anti-inflammatory surface modification strategy for vascular biomaterials.

Wound healing of nanofiber comprising Polygalacturonic/Hyaluronic acid embedded silver nanoparticles: In-vitro and in-vivo studies

2020 ◽  
Vol 238 ◽  
pp. 116175 ◽  
Author(s):  
M.R. El-Aassar ◽  
Omar M. Ibrahim ◽  
Moustafa M.G. Fouda ◽  
Nagham G. El-Beheri ◽  
Mona M. Agwa
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Hyaluronic Acid ◽  
In Vivo Studies

scholarly journals In Situ Cross-linking Hydrogel as a Vehicle for Retinal Progenitor Cell Transplantation

2019 ◽  
Vol 28 (5) ◽  
pp. 596-606 ◽  
Author(s):  
Jeayoung Park ◽  
Petr Baranov ◽  
Aybike Aydin ◽  
Hany Abdelgawad ◽  
Deepti Singh ◽  
...  
Keyword(s):  
In Vivo Studies ◽  
Subretinal Space ◽  
Cross Linking ◽  
Post Transplantation ◽  
Ki 67 ◽  
Retinal Progenitor ◽  
Stem Cell Delivery

One of the current limitations of retinal transplantation of stem cells as well as other cell types is the dispersion of cells from the injection site (including loss of cells into the vitreous chamber) and low survival after transplantation. Gelatin-hydroxyphenyl propionic acid (Gtn-HPA) conjugate is a biodegradable polymer that can undergo covalent cross-linking in situ, allowing for injection of incorporated cells through a small caliber needle followed by gel formation in vivo. We tested the hypothesis that Gtn-HPA hydrogel supports survival and integration of retinal progenitor cells (RPCs) post-transplantation. In vitro compatibility and in vivo graft survival were assessed by mixing an equal volume of Gtn-HPA conjugate and RPC suspension and triggering enzyme-mediated gelation, using minute amounts of horseradish peroxidase and peroxide. Immunocytochemistry showed >80% survival of cells and minimal apoptosis for cells incorporated into Gtn-HPA, equivalent to controls grown on fibronectin-coated flasks. RPCs undergoing mitosis were seen within the three-dimensional Gtn-HPA hydrogel, but the percentage of Ki-67-positive cells was lower compared with the monolayer controls. For in vivo studies, gel–cell mixture or cell suspension in saline was trans-sclerally injected into the left eye of female Long Evans rats immunosuppressed with cyclosporine A. Grafts survived at the 1 week time point of the study, with Gtn-HPA-delivered grafts showing less inflammatory response demonstrated by anti-leukocyte staining. More eyes in the gel–cell mixture group showed surviving cells in the subretinal space compared with saline-delivered controls, while the number of cells surviving per graft was not significantly different between the two groups. This work demonstrates an injectable in situ cross-linking hydrogel as a potential vehicle for stem cell delivery in the retina.

Quantitative Lung Ultrasound: Technical Aspects and Clinical Applications

2021 ◽  
Author(s):  
Silvia Mongodi ◽  
Daniele De Luca ◽  
Andrea Colombo ◽  
Andrea Stella ◽  
Erminio Santangelo ◽  
...  
Keyword(s):  
Lung Ultrasound ◽  
Scoring Systems ◽  
Clinical Applications ◽  
In Vivo Studies ◽  
Technical Aspects ◽  
Critical Care Units ◽  
Medical Wards ◽  
Opening Up

Lung ultrasound is increasingly used in emergency departments, medical wards, and critical care units—adult, pediatric, and neonatal. In vitro and in vivo studies show that the number and type of artifacts visualized change with lung density. This has led to the idea of a quantitative lung ultrasound approach, opening up new prospects for use not only as a diagnostic but also as a monitoring tool. Consequently, the multiple scoring systems proposed in the last few years have different technical approaches and specific clinical indications, adaptable for more or less time-dependent patients. However, multiple scoring systems may generate confusion among physicians aiming at introducing lung ultrasound in their clinical practice. This review describes the various lung ultrasound scoring systems and aims to clarify their use in different settings, focusing on technical aspects, validation with reference techniques, and clinical applications.

Sodium 3-mercaptopropanesulphonate substituted phthalocyanine: Synthesis, photophysical properties, in vitro and in vivo PDT efficacy

2020 ◽  
pp. A-E
Author(s):  
Mack Biyiklioglu
Keyword(s):  
Tumor Growth ◽  
Singlet Oxygen ◽  
Hepg2 Cells ◽  
Photophysical Properties ◽  
Clinical Applications ◽  
In Vivo Studies ◽  
Cremophor El ◽  
Photochemical Properties

A new sulfonic zinc(II) phthalocyanine bearing sodium 3-mercaptopropanesulphonate (Pc) was synthesized and characterized, as to its photophysical and photochemical properties, in vitro and in vivo. Pc remain non-aggregated in [Formula: see text],[Formula: see text]-dimethylformamide and in water containing 0.1% Cremophor EL, with high singlet oxygen efficacy. In vitro studies showed that the IC[Formula: see text] value of Pc on HepG2 cells was 1.3 [Formula: see text]M. In addition, in vivo studies showed that Pc mainly accumulated in tumor sites and showed an obvious PDT effect, and ca.97% of tumor growth was inhibited. Therefore, the Pc could be applied as a very promising photosensitizer for PDT in future clinical applications.

Betulin silver nanoparticles qualify as efficient antimelanoma agents in in vitro and in vivo studies

2019 ◽  
Vol 134 ◽  
pp. 1-19 ◽  
Author(s):  
Corina Danciu ◽  
Iulia Pinzaru ◽  
Dorina Coricovac ◽  
Florina Andrica ◽  
Ioana Sizemore ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
In Vivo Studies

scholarly journals Antimicrobial and antibiofilm potentials of cinnamon oil and silver nanoparticles against Streptococcus agalactiae isolated from bovine mastitis: new avenues for countering resistance

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Norhan K. Abd El-Aziz ◽  
Ahmed M. Ammar ◽  
El-sayed Y. M. El-Naenaeey ◽  
Hend M. El Damaty ◽  
Asmaa A. Elazazy ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Streptococcus Agalactiae ◽  
Quantitative Polymerase Chain Reaction ◽  
Bovine Mastitis ◽  
Dairy Farms ◽  
Clinical Mastitis ◽  
In Vivo Studies ◽  
Cinnamon Oil

Abstract Background Streptococcus agalactiae (S. agalactiae) is a contagious pathogen of bovine mastitis. It has financial implications for the dairy cattle industry in certain areas of the world. Since antimicrobial resistance increases in dairy farms, natural antimicrobials from herbal origins and nanoparticles have been given more attention as an alternative therapy. Hence, this study reported the antimicrobial and antibiofilm potentials of cinnamon oil, silver nanoparticles (AgNPs), and their combination against multidrug-resistant (MDR) S. agalactiae recovered from clinical bovine mastitis in Egypt. Results Our findings revealed that 73% (146/200) of the examined milk samples collected from dairy cows with clinical mastitis were infected with Streptococci species. Of these, 9.59% (14/146) were identified as S. agalactiae and categorized as MDR. S. agalactiae isolates expressed four virulence genes (Hyl, cylE, scpB, and lmb) and demonstrated an ability to produce biofilms. Cinnamon oil showed high antimicrobial (MICs ≤0.063 μg /mL) and antibiofilm (MBIC50 = 4 μg/mL) potentials against planktonic and biofilms of S. agalactiae isolates, respectively. However, AgNPs showed reasonable antimicrobial (MICs ≤16 μg/mL) and relatively low antibiofilm (MBIC50 = 64 μg/mL) activities against screened isolates. Synergistic antimicrobial or additive antibiofilm interactions of cinnamon oil combined with AgNPs were reported for the first time. Scanning electron microscope (SEM) analysis revealed that biofilms of S. agalactiae isolates treated with cinnamon oil were more seriously damaged than observed in AgNPs cinnamon oil combination. Moreover, reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) showed that cinnamon oil exerted a remarkable down-regulation of pili biosynthesis genes (pilA and pilB) and their regulator (rogB) against S. agalactiae biofilms, meanwhile the AgNPs cinnamon oil combination demonstrated a lower efficacy. Conclusions This is an in vitro preliminary approach that documented the antibiofilm potential of cinnamon oil and the inhibitory activity of cinnamon oil and its combination with AgNPs against MDR S. agalactiae recovered from clinical mastitis. Further in vivo studies should be carried out in animal models to provide evidence of concept for implementing these alternative candidates in the treatment of dairy farms infected by streptococcal mastitis in the future.

Sign in / Sign up

Export Citation Format

Share Document