scholarly journals Gelation properties of self-assembling N-acyl modified cytidine derivatives

2014 ◽  
Vol 2 (47) ◽  
pp. 8412-8417 ◽  
Author(s):  
K. J. Skilling ◽  
A. Ndungu ◽  
B. Kellam ◽  
M. Ashford ◽  
T. D. Bradshaw ◽  
...  
Keyword(s):  
Binary Mixture ◽  
Organic Solvent ◽  
Self Assembling ◽  
Delivery Platform

A new design for a self-assembling gelator of cytidine containing a binary mixture of organic solvent and water, shown to provide a suitable delivery platform for high and low Mw molecules.

scholarly journals Towards Developing Bioresponsive, Self-Assembled Peptide Materials: Dynamic Morphology and Fractal Nature of Nanostructured Matrices

Materials ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 1539 ◽  
Author(s):  
Kyle Koss ◽  
Larry Unsworth
Keyword(s):  
Fine Structure ◽  
Focal Point ◽  
Peptide Delivery ◽  
Bioactive Molecules ◽  
Matrix Metalloproteinase 2 ◽  
Fractal Nature ◽  
Self Assembling ◽  
Delivery Platform ◽  
Peptide Materials ◽  
Novel Strategy

(Arginine-alanine-aspartic acid-alanine)4 ((RADA)4) nanoscaffolds are excellent candidates for use as peptide delivery vehicles: they are relatively easy to synthesize with custom bio-functionality, and assemble in situ to allow a focal point of release. This enables (RADA)4 to be utilized in multiple release strategies by embedding a variety of bioactive molecules in an all-in-one “construct”. One novel strategy focuses on the local, on-demand release of peptides triggered via proteolysis of tethered peptide sequences. However, the spatial-temporal morphology of self-assembling nanoscaffolds may greatly influence the ability of enzymes to both diffuse into as well as actively cleave substrates. Fine structure and its impact on the overall effect on peptide release is poorly understood. In addition, fractal networks observed in nanoscaffolds are linked to the fractal nature of diffusion in these systems. Therefore, matrix morphology and fractal dimension of virgin (RADA)4 and mixtures of (RADA)4 and matrix metalloproteinase 2 (MMP-2) cleavable substrate modified (RADA)4 were characterized over time. Sites of high (glycine-proline-glutamine-glycine+isoleucine-alanine-serine-glutamine (GPQG+IASQ), CP1) and low (glycine-proline-glutamine-glycine+proline-alanine-glycine-glutamine (GPQG+PAGQ), CP2) cleavage activity were chosen. Fine structure was visualized using transmission electron microscopy. After 2 h of incubation, nanofiber networks showed an established fractal nature; however, nanofibers continued to bundle in all cases as incubation times increased. It was observed that despite extensive nanofiber bundling after 24 h of incubation time, the CP1 and CP2 nanoscaffolds were susceptible to MMP-2 cleavage. The properties of these engineered nanoscaffolds characterized herein illustrate that they are an excellent candidate as an enzymatically initiated peptide delivery platform.

Using organic solvent absorption as a self-assembly method to synthesize three-dimensional (3D) reduced graphene oxide (RGO)/poly(3,4-ethylenedioxythiophene) (PEDOT) architecture and its electromagnetic absorption properties

RSC Advances ◽  
2014 ◽  
Vol 4 (91) ◽  
pp. 49780-49782 ◽  
Author(s):  
Fan Wu ◽  
Yuan Wang ◽  
Mingyang Wang
Keyword(s):  
Organic Solvent ◽  
Self Assembly ◽  
Three Dimensional ◽  
Absorption Properties ◽  
Self Assembling ◽  
Assembly Method ◽  
Electromagnetic Absorption ◽  
Solvent Absorption ◽  
Reduced Graphene ◽  
Promising Application

A novel self-assembling 3D-RGO/PEDOT architecture has been synthesized through organic solvent absorption and gentle heating. It gives a promising application in electromagnetic absorption.

scholarly journals Towards Developing Bioresponsive, Self-Assembled Peptide Materials: Dynamic Morphology and Fractal Nature of Nanostructured Matrices

2018 ◽  
Author(s):  
Kyle Koss ◽  
Larry Unsworth
Keyword(s):  
Fine Structure ◽  
Focal Point ◽  
Peptide Delivery ◽  
Bioactive Molecules ◽  
Matrix Metalloproteinase 2 ◽  
Fractal Nature ◽  
Self Assembling ◽  
Delivery Platform ◽  
Peptide Materials ◽  
Novel Strategy

(RADA)4 nanoscaffolds are excellent candidates for use as peptide delivery vehicles: they are relatively easy to synthesize with custom bio-functionality, and assemble in situ to allow a focal point of release. This enables (RADA)4 to be utilized in multiple release strategies by embedding a variety of bioactive molecules in an all-in-one ‘construct’. One novel strategy focuses on the local, on-demand release of peptides triggered via proteolysis of tethered peptide sequences. However, the spatial-temporal morphology of self-assembling nanoscaffolds may greatly influence the ability for enzymes to both diffuse into as well as actively cleave substrates. Fine structure and its impact on overall affect on peptide release is poorly understood. In addition, fractal networks observed in nanoscaffolds are linked to the fractal nature of diffusion in these systems. Therefore, matrix morphology and fractal dimension of virgin (RADA)4 and mixtures of (RADA)4 and matrix metalloproteinase 2 (MMP-2) cleavable substrate modified (RADA)4 were characterized over time. Sites of high (GPQG+IASQ, CP1) and low (GPQG+PAGQ, CP2) cleavage activity were chosen. Fine structure was visualized using established according to established methods. After 2 hrs of incubation, nanofiber networks showed an established fractal nature, however nanofibers continued to bundle in all cases as incubation times increased. It was observed that despite extensive nanofiber bundling after 24 hrs of incubation time, the CP1 and CP2 nanoscaffolds were susceptible to MMP-2 cleavage. The properties of these engineered nanoscaffolds characterized herein illustrate that they are an excellent candidate as an enzymatically initiated peptide delivery platform.

scholarly journals Polypeptide/Doxorubicin Hydrochloride Polymersomes Prepared Through Organic Solvent-free Technique as a Smart Drug Delivery Platform

2013 ◽  
Vol 13 (9) ◽  
pp. 1150-1162 ◽  
Author(s):  
Mingqiang Li ◽  
Shixian Lv ◽  
Zhaohui Tang ◽  
Wantong Song ◽  
Haiyang Yu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Organic Solvent ◽  
Solvent Free ◽  
Doxorubicin Hydrochloride ◽  
Delivery Platform ◽  
Smart Drug ◽  
Smart Drug Delivery

Self-Assembling Peptide Nanofibrous Hydrogel as a Versatile Drug Delivery Platform

2015 ◽  
Vol 21 (29) ◽  
pp. 4342-4354 ◽  
Author(s):  
Zhiqiang Yu ◽  
Quan Xu ◽  
Chenbo Dong ◽  
Su Lee ◽  
Liqian Gao ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Self Assembling ◽  
Delivery Platform

scholarly journals Orientation of Antigen Display on Self-Assembling Protein Nanoparticles Influences Immunogenicity

Vaccines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 103
Author(s):  
Cosette G. Schneider ◽  
Justin A. Taylor ◽  
Michael Q. Sibilo ◽  
Kazutoyo Miura ◽  
Katherine L. Mallory ◽  
...  
Keyword(s):  
Surface Protein ◽  
Merozoite Surface Protein ◽  
Effective Vaccine ◽  
Antigen Delivery ◽  
Self Assembling ◽  
Protein Nanoparticles ◽  
Delivery Platform ◽  
Dual Stage ◽  
Functional Antibodies ◽  
Stage Parasite

Self-assembling protein nanoparticles (SAPN) serve as a repetitive antigen delivery platform with high-density epitope display; however, antigen characteristics such as size and epitope presentation can influence the immunogenicity of the assembled particle and are aspects to consider for a rationally designed effective vaccine. Here, we characterize the folding and immunogenicity of heterogeneous antigen display by integrating (a) dual-stage antigen SAPN presenting the P. falciparum (Pf) merozoite surface protein 1 subunit, PfMSP119, and Pf cell-traversal protein for ookinetes and sporozoites, PfCelTOS, in addition to (b) a homogenous antigen SAPN displaying two copies of PfCelTOS. Mice and rabbits were utilized to evaluate antigen-specific humoral and cellular induction as well as functional antibodies via growth inhibition of the blood-stage parasite. We demonstrate that antigen orientation and folding influence the elicited immune response, and when appropriately designed, SAPN can serve as an adaptable platform for an effective multi-antigen display.

scholarly journals Vaccine Self-Assembling Immune Matrix Is a New Delivery Platform That Enhances Immune Responses to Recombinant HBsAg in Mice

2015 ◽  
Vol 22 (3) ◽  
pp. 336-343 ◽  
Author(s):  
Rafaella F. Q. Grenfell ◽  
Lisa M. Shollenberger ◽  
E. Farah Samli ◽  
Donald A. Harn
Keyword(s):  
Delivery Method ◽  
Proinflammatory Response ◽  
Virus Surface ◽  
Self Assembling ◽  
B Virus ◽  
Delivery Platform ◽  
Specific Igg ◽  
Recombinant Hbsag ◽  
Antigen Presenting ◽  
Effective Public Health

ABSTRACTVaccination remains the most effective public health tool to prevent infectious diseases. Many vaccines are marginally effective and need enhancement for immunocompromised, elderly, and very young populations. To enhance immunogenicity, we exploited the biphasic property of the (RADA)4 synthetic oligopeptide to create VacSIM (vaccine self-assembling immune matrix), a new delivery method. VacSIM solution can easily be mixed with antigens, organisms, and adjuvants for injection. Postinjection, the peptides self-assemble into hydrated nanofiber gel matrices, forming a depot with antigens and adjuvants in the aqueous phase. We believe the depot provides slow release of immunogens, leading to increased activation of antigen-presenting cells that then drive enhanced immunogenicity. Using recombinant hepatitis B virus surface antigen (rHBsAg) as a model immunogen, we compared VacSIM delivery to delivery in alum or complete Freund's adjuvant (CFA). Delivery of the rHBsAg antigen to mice via VacSIM without adjuvant elicited higher specific IgG responses than when rHBsAg was delivered in alum or CFA. Evaluating IgG subtypes showed a mixed Th1/Th2 type response following immunization with VacSIM, which was driven further toward Th1 with addition of CpG as the adjuvant. Increased specific IgG endpoint titers were observed in both C57BL/6 and BALB/c mice, representative of Th1 and Th2 environments, respectively. Restimulation of splenocytes suggests that VacSIM does not cause an immediate proinflammatory response in the host. Overall, these results suggest that VacSIM, as a new delivery method, has the potential to enhance immunogenicity and efficacy of numerous vaccines.

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