Engineered PEI-piperazinyl nanoparticles as efficient gene delivery vectors: evidence from both in vitro and in vivo studies

RSC Advances ◽  
2012 ◽  
Vol 2 (10) ◽  
pp. 4335 ◽  
Author(s):  
Soma Patnaik ◽  
Ritu Goyal ◽  
Sushil K. Tripathi ◽  
Mohammed Arif ◽  
Kailash C. Gupta
Keyword(s):  
Gene Delivery ◽  
In Vivo Studies ◽  
Gene Delivery Vectors ◽  
Efficient Gene

Gellan gum blended PEI nanocomposites as gene delivery agents: Evidences from in vitro and in vivo studies

2011 ◽  
Vol 79 (1) ◽  
pp. 3-14 ◽  
Author(s):  
Ritu Goyal ◽  
S.K. Tripathi ◽  
Shilpa Tyagi ◽  
K. Ravi Ram ◽  
K.M. Ansari ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Gellan Gum ◽  
In Vivo Studies

scholarly journals Aromatic Thioacetal-Bridged ROS-Responsive Nanoparticles as Novel Gene Delivery Vehicles

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2061 ◽  
Author(s):  
Guo-Qing Lin ◽  
Wen-Jing Yi ◽  
Qiang Liu ◽  
Xue-Jun Yang ◽  
Zhi-Gang Zhao
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Zeta Potentials ◽  
Sar Studies ◽  
Gene Delivery Vectors ◽  
Efficient Gene ◽  
Delivery Vehicles ◽  
Low Cytotoxicity

In this report, a series of polycations are designed and synthesized by conjugating reactive oxygen species (ROS)-responsive thioacetal-linkers to low molecular weight (LMW) polyethylenimine (PEI) via ring-opening polymerization. Their structure–activity relationships (SARs) as gene delivery vectors are systematically studied. Although the MWs of the target polymers are only ~9 KDa, they show good DNA binding ability. The formed polyplexes, which are stable toward serum but decomposed under ROS-conditions, have appropriate sizes (180~300 nm) and positive zeta-potentials (+35~50 mV). In vitro experiments reveal that these materials have low cytotoxicity, and higher transfection efficiency (TE) than controls. Furthermore, the title polymers exhibit excellent serum tolerance. With the present of 10% serum, the TE of the polymers even increases up to 10 times higher than 25 KDa PEI and 9 times higher than Lipofectamine 2000. The SAR studies also reveal that electron-withdrawing groups on the aromatic ring in 4a may benefit to balance between the DNA condensation and release for efficient gene transfection.

Poly(ethylene glycol)-poly-l-glutamate complexed with polyethyleneimine−polyglycine for highly efficient gene delivery in vitro and in vivo

2018 ◽  
Vol 6 (11) ◽  
pp. 3053-3062 ◽  
Author(s):  
Zhaopei Guo ◽  
Lin Lin ◽  
Jie Chen ◽  
Xingzhi Zhou ◽  
Hon Fai Chan ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Gene Delivery ◽  
Ethylene Glycol ◽  
Gene Delivery System ◽  
Poly Ethylene Glycol ◽  
Highly Efficient ◽  
Efficient Gene ◽  
Poly Ethylene

The highly efficient gene delivery system with effective serum resistant capacity is promising for cancer therapy.

scholarly journals A Valid Bisphosphonate Modified Calcium Phosphate-Based Gene Delivery System: Increased Stability and Enhanced Transfection Efficiency In Vitro and In Vivo

Pharmaceutics ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 468 ◽  
Author(s):  
Zhao ◽  
Li ◽  
Chen ◽  
Hu
Keyword(s):  
Calcium Phosphate ◽  
Gene Delivery ◽  
Tissue Specificity ◽  
Transfection Efficiency ◽  
Hybrid Nanoparticles ◽  
In Vivo Studies ◽  
Adsorptive Capacity ◽  
Low Cytotoxicity

Calcium phosphate (CaP) nanoparticles, as a promising vehicle for gene delivery, have been widely used owing to their biocompatibility, biodegradability and adsorptive capacity for nucleic acids. Unfortunately, their utility in vivo has been profoundly restricted due to numerous technical barriers such as the lack of tissue specificity and limited transfection efficiency, as well as uncontrollable aggregation over time. To address these issues, an effective conjugate folate-polyethylene glycol-pamidronate (shortened as FA-PEG-Pam) was designed and coated on the surface of CaP/NLS/pDNA (CaP/NDs), forming a versatile gene carrier FA-PEG-Pam/CaP/NDs. Inclusion of FA-PEG-Pam significantly reduced the size of CaP nanoparticles, thus inhibiting the aggregation of CaP nanoparticles. FA-PEG-Pam/CaP/NDs showed better cellular uptake than mPEG-Pam/CaP/NDs, which could be attributed to the high-affinity interactions between FA and highly expressed FR. Meanwhile, FA-PEG-Pam/CaP/NDs had low cytotoxicity and desired effect on inducing apoptosis (71.1%). Furthermore, FA-PEG-Pam/CaP/NDs showed admirable transfection efficiency (63.5%) due to the presence of NLS peptides. What’s more, in vivo studies revealed that the hybrid nanoparticles had supreme antitumor activity (IR% = 58.7%) among the whole preparations. Altogether, FA-PEG-Pam/CaP/NDs was expected to be a hopeful strategy for gene delivery.

Peptide-Based Vector of VEGF Plasmid for Efficient Gene Delivery in Vitro and Vessel Formation in Vivo

2013 ◽  
Vol 24 (6) ◽  
pp. 960-967 ◽  
Author(s):  
Wei Qu ◽  
Si-Yong Qin ◽  
Shan Ren ◽  
Xue-Jun Jiang ◽  
Ren-Xi Zhuo ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Vessel Formation ◽  
Efficient Gene
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