scholarly journals ROS-cleavable proline oligomer crosslinking of polycaprolactone for pro-angiogenic host response

2014 ◽  
Vol 2 (41) ◽  
pp. 7109-7113 ◽  
Author(s):  
Sue Hyun Lee ◽  
Timothy C. Boire ◽  
Jung Bok Lee ◽  
Mukesh K. Gupta ◽  
Angela L. Zachman ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Host Response ◽  
Porous Scaffolds ◽  
Engineering Applications ◽  
Vessel Growth

When carboxylated poly(ε-caprolactone) (PCL) is crosslinked with ROS-degradable peptide KP7K oligomers and fabricated into porous scaffolds for tissue engineering applications, the scaffolds exhibit excellent physiological ROS-mediated degradation with induction of new blood vessel growth from the host.

scholarly journals Sustained VEGF delivery via PLGA nanoparticles promotes vascular growth

2010 ◽  
Vol 298 (6) ◽  
pp. H1959-H1965 ◽  
Author(s):  
Justin S. Golub ◽  
Young-tae Kim ◽  
Craig L. Duvall ◽  
Ravi V. Bellamkonda ◽  
Divya Gupta ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Femoral Artery ◽  
Release Kinetics ◽  
Aortic Ring ◽  
Plga Nanoparticles ◽  
Cardiovascular Medicine ◽  
Vessel Growth ◽  
Vessel Volume

Technologies to increase tissue vascularity are critically important to the fields of tissue engineering and cardiovascular medicine. Currently, limited technologies exist to encourage angiogenesis and arteriogenesis in a controlled manner. In the present study, we describe an injectable controlled release system consisting of VEGF encapsulated in poly(lactic- co-glycolic acid) (PLGA) nanoparticles (NPs). The majority of VEGF was released gradually over 2–4 days from the NPs as determined by an ELISA release kinetics experiment. An in vitro aortic ring bioassay was used to verify the bioactivity of VEGF-NPs compared with empty NPs and no treatment. A mouse femoral artery ischemia model was then used to measure revascularization in VEGF-NP-treated limbs compared with limbs treated with naked VEGF and saline. 129/Sv mice were anesthetized with isoflurane, and a region of the common femoral artery and vein was ligated and excised. Mice were then injected with VEGF-NPs, naked VEGF, or saline. After 4 days, three-dimensional microcomputed tomography angiography was used to quantify vessel growth and morphology. Mice that received VEGF-NP treatment showed a significant increase in total vessel volume and vessel connectivity compared with 5 μg VEGF, 2.5 μg VEGF, and saline treatment (all P < 0.001). When the yield of the fabrication process was taken into account, VEGF-NPs were over an order of magnitude more potent than naked VEGF in increasing blood vessel volume. Differences between the VEGF-NP group and all other groups were even greater when only small-sized vessels under 300 μm diameter were analyzed. In conclusion, sustained VEGF delivery via PLGA NPs shows promise for encouraging blood vessel growth in tissue engineering and cardiovascular medicine applications.

Fabrication of alumina porous scaffolds with aligned oriented pores for bone tissue engineering applications

2016 ◽  
Vol 122 (4) ◽  
Author(s):  
Fatemeh Sarhadi ◽  
Mahdi Shafiee Afarani ◽  
Davod Mohebbi-Kalhori ◽  
Masoud Shayesteh
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffolds ◽  
Engineering Applications

scholarly journals Antioxidant, and enhanced flexible nano porous scaffolds for bone tissue engineering applications

Nano Select ◽  
2021 ◽  
Author(s):  
Muhammad Sohail Asghar ◽  
Jinhua Li ◽  
Iftikhar Ahmed ◽  
Uzma Ghazanfar ◽  
Muhammad Sultan Irshad ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffolds ◽  
Engineering Applications

scholarly journals Synthetic Biodegradable Aliphatic Polyester Nanocomposites Reinforced with Nanohydroxyapatite and/or Graphene Oxide for Bone Tissue Engineering Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 590 ◽  
Author(s):  
Yuchao Li ◽  
Chengzhu Liao ◽  
Sie Chin Tjong
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Porous Scaffolds ◽  
Aliphatic Polyester ◽  
Engineering Applications ◽  
Aliphatic Polyesters ◽  
Nanocomposite Scaffolds

This paper provides review updates on the current development of bionanocomposites with polymeric matrices consisting of synthetic biodegradable aliphatic polyesters reinforced with nanohydroxyaptite (nHA) and/or graphene oxide (GO) nanofillers for bone tissue engineering applications. Biodegradable aliphatic polyesters include poly(lactic acid) (PLA), polycaprolactone (PCL) and copolymers of PLA-PGA (PLGA). Those bionanocomposites have been explored for making 3D porous scaffolds for the repair of bone defects since nHA and GO enhance their bioactivity and biocompatibility by promoting biomineralization, bone cell adhesion, proliferation and differentiation, thus facilitating new bone tissue formation upon implantation. The incorporation of nHA or GO into aliphatic polyester scaffolds also improves their mechanical strength greatly, especially hybrid GO/nHA nanofilllers. Those mechanically strong nanocomposite scaffolds can support and promote cell attachment for tissue growth. Porous scaffolds fabricated from conventional porogen leaching, and thermally induced phase separation have many drawbacks inducing the use of organic solvents, poor control of pore shape and pore interconnectivity, while electrospinning mats exhibit small pores that limit cell infiltration and tissue ingrowth. Recent advancement of 3D additive manufacturing allows the production of aliphatic polyester nanocomposite scaffolds with precisely controlled pore geometries and large pores for the cell attachment, growth, and differentiation in vitro, and the new bone formation in vivo.

Reinforced Portland cement porous scaffolds for load-bearing bone tissue engineering applications

2011 ◽  
Vol 100B (2) ◽  
pp. 501-507 ◽  
Author(s):  
Natalia Higuita-Castro ◽  
Daniel Gallego-Perez ◽  
Alejandro Pelaez-Vargas ◽  
Felipe García Quiroz ◽  
Olga M. Posada ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Portland Cement ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffolds ◽  
Engineering Applications ◽  
Load Bearing

scholarly journals Tissue Engineering 3D Porous Scaffolds Prepared from Electrospun Recombinant Human Collagen (RHC) Polypeptides/Chitosan Nanofibers

2021 ◽  
Vol 11 (11) ◽  
pp. 5096
Author(s):  
Aipeng Deng ◽  
Yang Yang ◽  
Shimei Du
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Great Promise ◽  
Porous Scaffolds ◽  
High Porosity ◽  
Engineering Applications ◽  
Nanofibrous Scaffolds ◽  
Human Collagen ◽  
Hierarchical Pore ◽  
Nih 3T3

Electrospinning, the only method that can continuously produce nanofibers, has been widely used to prepare nanofibers for tissue engineering applications. However, electrospinning is not suitable for preparing clinically relevant three-dimensional (3D) nanofibrous scaffolds with hierarchical pore structures. In this study, recombinant human collagen (RHC)/chitosan nanofibers prepared by electrospinning were combined with porous scaffolds produced by freeze drying to fabricate 3D nanofibrous scaffolds. These scaffolds exhibited high porosity (over 80%) and an interconnected porous structure (ranging from sub-micrometers to 200 μm) covered with nanofibers. As confirmed by the characterization results, these scaffolds showed good swelling ability, stability, and adequate mechanical strength, making it possible to use the 3D nanofibrous scaffolds in various tissue engineering applications. In addition, after seven days of cell culturing, NIH 3T3 was infiltrated into the scaffolds while maintaining its morphology and with superior proliferation and viability. These results indicated that the 3D nanofibrous scaffolds hold great promise for tissue engineering applications.

scholarly journals Structural crystallisation of crosslinked 3D PEDOT:PSS anisotropic porous biomaterials to generate highly conductive platforms for tissue engineering applications

2021 ◽  
Author(s):  
Matteo Solazzo ◽  
Michael G. Monaghan
Keyword(s):  
Tissue Engineering ◽  
Sulphuric Acid ◽  
Porous Scaffolds ◽  
Engineering Applications ◽  
New Approach ◽  
Porous Biomaterials

A new approach for obtaining highly conductive, yet biocompatible, 3D electroconductive porous scaffolds based on PEDOT:PSS and treated with sulphuric acid crystallisation, that can be processed with both isotropic and anisotropic microarchitecture.

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