scholarly journals Core–shell structured phosphorescent nanoparticles for detection of exogenous and endogenous hypochlorite in live cells via ratiometric imaging and photoluminescence lifetime imaging microscopy

2015 ◽  
Vol 6 (1) ◽  
pp. 301-307 ◽  
Author(s):  
Kenneth Yin Zhang ◽  
Jie Zhang ◽  
Yahong Liu ◽  
Shujuan Liu ◽  
Pinglin Zhang ◽  
...  

Core-shell phosphorescent nanoparticles were used to detect intracellular ClO− via ratiometric and photoluminescence lifetime imaging.

2015 ◽  
Vol 6 (3) ◽  
pp. 1825-1831 ◽  
Author(s):  
Qiang Zhao ◽  
Xiaobo Zhou ◽  
Tianye Cao ◽  
Kenneth Yin Zhang ◽  
Lijuan Yang ◽  
...  

Fluorescent/phosphorescent dual-emissive conjugated polymer dots were designed and synthesized, ere used for tumor hypoxia sensing via ratiometric imaging and photoluminescence lifetime imaging.


2021 ◽  
Vol 93 (5) ◽  
pp. 2988-2995
Author(s):  
Zihe Yan ◽  
Jianfeng Xue ◽  
Min Zhou ◽  
Jinyu Wang ◽  
Yanxin Zhang ◽  
...  

2017 ◽  
Author(s):  
Alice Sherrard ◽  
Paul Bishop ◽  
Melanie Panagi ◽  
Maria Beatriz Villagomez ◽  
Dominic Alibhai ◽  
...  

AbstractChanges in chromatin compaction are crucial during genomic responses. Thus, methods that enable such measurements are instrumental for investigating genome function. Here, we address this challenge by developing, validating, and streamlining histone-based fluorescence lifetime imaging microscopy (FLIM) that robustly detects chromatin compaction states in fixed and live cells; in 2D and 3D. We present quality-controlled and detailed method that is simpler and faster than previous approches, and uses FLIMfit open-source software. We demonstrate the versatility of our method through its combination with immunofluorescence and its implementation in immortalised cells and primary neurons. Owing to these developments, we applied this method to elucidate the function of the DNA damage response kinase, ATM, in regulating chromatin organisation after genotoxic-stress. We unravelled a role for ATM in regulating chromatin compaction independently of DNA damage. Collectively, we present an adaptable chromatin FLIM method for examining chromatin structure in cells, and establish its broader utility.


2015 ◽  
Vol 51 (62) ◽  
pp. 12447-12450 ◽  
Author(s):  
Gang Fan ◽  
Yao-Xin Lin ◽  
Le Yang ◽  
Fu-Ping Gao ◽  
Ying-Xi Zhao ◽  
...  

Co-self-assembled vesicular nanoparticles of two structurally comparable amphiphilic boron-dipyrromethene (BODIPY) dyes with dequenchable dual colour fluorescence were prepared for ratiometric imaging of live cells.


2014 ◽  
Vol 3 (3) ◽  
pp. 211-218 ◽  
Author(s):  
Kathrin M. Scherer ◽  
Roger H. Bisby ◽  
Stanley W. Botchway ◽  
Greg M. Greetham ◽  
John A. Hadfield ◽  
...  

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