scholarly journals Thiazole orange-conjugated peptide nucleic acid for fluorescent detection of specific DNA sequences and site-selective photodamage

RSC Advances ◽  
2014 ◽  
Vol 4 (108) ◽  
pp. 63533-63538 ◽  
Author(s):  
Makiko Tanaka ◽  
Narumi Shigi ◽  
Jun Sumaoka ◽  
Makoto Komiyama
Keyword(s):  
Nucleic Acid ◽  
Dna Sequences ◽  
Peptide Nucleic Acid ◽  
Fluorescent Detection ◽  
Thiazole Orange ◽  
Site Selective

Conjugates of thiazole orange (TO) with a pseudo-complementary peptide nucleic acid (pcPNA) functioned as (i) fluorescent detector of specific DNA and (ii) site-selective photodamage inducer through generation of 1O2.

Real time RNA transcription monitoring by Thiazole Orange (TO)-conjugated Peptide Nucleic Acid (PNA) probes: norovirus detection

2011 ◽  
Vol 7 (5) ◽  
pp. 1684 ◽  
Author(s):  
Alessandro Tonelli ◽  
Tullia Tedeschi ◽  
Andrea Germini ◽  
Stefano Sforza ◽  
Roberto Corradini ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Peptide Nucleic Acid ◽  
Thiazole Orange ◽  
Rna Transcription

scholarly journals Electrospray ionisation-cleavable tandem nucleic acid mass tag–peptide nucleic acid conjugates: synthesis and applications to quantitative genomic analysis using electrospray ionisation-MS/MS

2006 ◽  
Vol 35 (4) ◽  
pp. e28-e28 ◽  
Author(s):  
Andrew Thompson ◽  
Mark Prescott ◽  
Noorhan Chelebi ◽  
John Smith ◽  
Tom Brown ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Sequences ◽  
Peptide Nucleic Acid ◽  
Ion Source ◽  
Esi Ms ◽  
Electrospray Ionisation ◽  
Hybridization Probes ◽  
Target Dna ◽  
Detection And Quantification ◽  
Mass Tag

Abstract The synthesis and characterization of isotopomer tandem nucleic acid mass tag–peptide nucleic acid (TNT–PNA) conjugates is described along with their use as electrospray ionisation-cleavable (ESI-Cleavable) hybridization probes for the detection and quantification of target DNA sequences by electrospray ionisation tandem mass spectrometry (ESI-MS/MS). ESI-cleavable peptide TNT isotopomers were introduced into PNA oligonucleotide sequences in a total synthesis approach. These conjugates were evaluated as hybridization probes for the detection and quantification of immobilized synthetic target DNAs using ESI-MS/MS. In these experiments, the PNA portion of the conjugate acts as a hybridization probe, whereas the peptide TNT is released in a collision-based process during the ionization of the probe conjugate in the electrospray ion source. The cleaved TNT acts as a uniquely resolvable marker to identify and quantify a unique target DNA sequence. The method should be applicable to a wide variety of assays requiring highly multiplexed, quantitative DNA/RNA analysis, including gene expression monitoring, genetic profiling and the detection of pathogens.

Site-Selective Binding of Nanoparticles to Double-Stranded DNA via Peptide Nucleic Acid “Invasion”

ACS Nano ◽  
2011 ◽  
Vol 5 (4) ◽  
pp. 2467-2474 ◽  
Author(s):  
Andrea L. Stadler ◽  
Dazhi Sun ◽  
Mathew M. Maye ◽  
Daniel van der Lelie ◽  
Oleg Gang
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Selective Binding ◽  
Double Stranded Dna ◽  
Site Selective

Site-selective DNA hydrolysis induced by a metal-free peptide nucleic acid–cyclen conjugate

2011 ◽  
Vol 47 (39) ◽  
pp. 11059 ◽  
Author(s):  
Ming-Qi Wang ◽  
Jun-Liang Liu ◽  
Jing-Yi Wang ◽  
Da-Wei Zhang ◽  
Ji Zhang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Dna Hydrolysis ◽  
Metal Free ◽  
Free Peptide ◽  
Site Selective

Synthetic fluorescent probes capable of selective recognition of 3′-overhanging nucleotides for siRNA delivery imaging

2015 ◽  
Vol 51 (8) ◽  
pp. 1421-1424 ◽  
Author(s):  
Takaya Sato ◽  
Yusuke Sato ◽  
Kenta Iwai ◽  
Shusuke Kuge ◽  
Seiichi Nishizawa ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Fluorescent Probes ◽  
Peptide Nucleic Acid ◽  
Sirna Delivery ◽  
Selective Recognition ◽  
Thiazole Orange ◽  
Accurate Analysis

Peptide nucleic acid (PNA)–thiazole orange (TO) conjugates are developed as fluorescent probes capable of selective recognition of 3′-overhanging nucleotides of siRNAs for an accurate analysis of the siRNA delivery process.

Synthesis and fluorescence properties of 3,6-diaminocarbazole-modified pyrrolidinyl peptide nucleic acid

RSC Advances ◽  
2016 ◽  
Vol 6 (78) ◽  
pp. 74314-74322 ◽  
Author(s):  
Aukkrapon Dangsopon ◽  
Nattawee Poomsuk ◽  
Khatcharin Siriwong ◽  
Tirayut Vilaivan ◽  
Chaturong Suparpprom
Keyword(s):  
Nucleic Acid ◽  
Dna Sequences ◽  
Peptide Nucleic Acid ◽  
Fluorescence Properties

Diaminocarbazole-labeled acpcPNA as a self-reporting probe for determination of DNA sequences.

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