scholarly journals Light-Up Probes: Thiazole Orange-Conjugated Peptide Nucleic Acid for Detection of Target Nucleic Acid in Homogeneous Solution

2000 ◽  
Vol 281 (1) ◽  
pp. 26-35 ◽  
Author(s):  
Nicke Svanvik ◽  
Gunnar Westman ◽  
Dongyuan Wang ◽  
Mikael Kubista
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Homogeneous Solution ◽  
Thiazole Orange ◽  
Target Nucleic Acid

Real time RNA transcription monitoring by Thiazole Orange (TO)-conjugated Peptide Nucleic Acid (PNA) probes: norovirus detection

2011 ◽  
Vol 7 (5) ◽  
pp. 1684 ◽  
Author(s):  
Alessandro Tonelli ◽  
Tullia Tedeschi ◽  
Andrea Germini ◽  
Stefano Sforza ◽  
Roberto Corradini ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Peptide Nucleic Acid ◽  
Thiazole Orange ◽  
Rna Transcription

scholarly journals Thiazole orange-conjugated peptide nucleic acid for fluorescent detection of specific DNA sequences and site-selective photodamage

RSC Advances ◽  
2014 ◽  
Vol 4 (108) ◽  
pp. 63533-63538 ◽  
Author(s):  
Makiko Tanaka ◽  
Narumi Shigi ◽  
Jun Sumaoka ◽  
Makoto Komiyama
Keyword(s):  
Nucleic Acid ◽  
Dna Sequences ◽  
Peptide Nucleic Acid ◽  
Fluorescent Detection ◽  
Thiazole Orange ◽  
Site Selective

Conjugates of thiazole orange (TO) with a pseudo-complementary peptide nucleic acid (pcPNA) functioned as (i) fluorescent detector of specific DNA and (ii) site-selective photodamage inducer through generation of 1O2.

Synthetic fluorescent probes capable of selective recognition of 3′-overhanging nucleotides for siRNA delivery imaging

2015 ◽  
Vol 51 (8) ◽  
pp. 1421-1424 ◽  
Author(s):  
Takaya Sato ◽  
Yusuke Sato ◽  
Kenta Iwai ◽  
Shusuke Kuge ◽  
Seiichi Nishizawa ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Fluorescent Probes ◽  
Peptide Nucleic Acid ◽  
Sirna Delivery ◽  
Selective Recognition ◽  
Thiazole Orange ◽  
Accurate Analysis

Peptide nucleic acid (PNA)–thiazole orange (TO) conjugates are developed as fluorescent probes capable of selective recognition of 3′-overhanging nucleotides of siRNAs for an accurate analysis of the siRNA delivery process.

Molecular insights on the dynamic stability of peptide nucleic acid functionalized carbon and boron nitride nanotubes

2021 ◽  
Vol 23 (1) ◽  
pp. 219-228
Author(s):  
Nabanita Saikia ◽  
Mohamed Taha ◽  
Ravindra Pandey
Keyword(s):  
Nucleic Acid ◽  
Boron Nitride ◽  
Nucleic Acids ◽  
Dynamic Stability ◽  
Peptide Nucleic Acid ◽  
Rational Design ◽  
Peptide Nucleic Acids ◽  
Boron Nitride Nanotubes ◽  
Molecular Hybrids ◽  
Single Wall Nanotubes

The rational design of self-assembled nanobio-molecular hybrids of peptide nucleic acids with single-wall nanotubes rely on understanding how biomolecules recognize and mediate intermolecular interactions with the nanomaterial's surface.

Design and in-silico screening of Peptide Nucleic Acid (PNA) inspired novel pronucleotide scaffolds targeting COVID-19

2020 ◽  
Vol 16 ◽  
Author(s):  
Bichismita Sahu ◽  
Santosh Kumar Behera ◽  
Rudradip Das ◽  
Tanay Dalvi ◽  
Arnab Chowdhury ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
In Silico ◽  
Peptide Nucleic Acid ◽  
Large Set ◽  
Nonstructural Proteins ◽  
In Silico Screening ◽  
Replication Process ◽  
Enveloped Virus ◽  
Treatment Regimens ◽  
Novel Coronavirus

Introduction: The outburst of the novel coronavirus COVID-19, at the end of December 2019 has turned itself into a pandemic taking a heavy toll on human lives. The causal agent being SARS-CoV-2, a member of the long-known Coronaviridae family, is a positive sense single-stranded enveloped virus and quite closely related to SARS-CoV. It has become the need of the hour to understand the pathophysiology of this disease, so that drugs, vaccines, treatment regimens and plausible therapeutic agents can be produced. Methods: In this regard, recent studies uncovered the fact that the viral genome of SARS-CoV-2 encodes nonstructural proteins like RNA dependent RNA polymerase (RdRp) which is an important tool for its transcription and replication process. A large number of nucleic acid based anti-viral drugs are being repurposed for treating COVID-19 targeting RdRp. Few of them are in the advanced stage of clinical trials including Remdesivir. While performing close investigation of the large set of nucleic acid based drugs, we were surprised to find that the synthetic nucleic acid backbone is explored very little or rare. Results: We have designed scaffolds derived from peptide nucleic acid (PNA) and subjected them for in-silico screening systematically. These designed molecules have demonstrated excellent binding towards RdRp. Compound 12 was found to possess similar binding affinity as Remdesivir with comparable pharmacokinetics. However, the in-silico toxicity prediction indicates compound 12 may be a superior molecule which can be explored further due to its excellent safety-profile with LD50 (12,000mg/kg) as opposed to Remdesivir (LD50 =1000mg/kg). Conclusion: Compound 12 falls in the safe category of class 6. Synthetic feasibility, equipotent binding and very low toxicity of this peptide nucleic acid derived compounds can serve as a leading scaffold to design, synthesize and evaluate many of similar compounds for the treatment of COVID-19.

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