Enzyme-mediated preparation of hydrogels composed of poly(ethylene glycol) and gelatin as cell culture platforms

RSC Advances ◽  
2015 ◽  
Vol 5 (4) ◽  
pp. 3070-3073 ◽  
Author(s):  
Kousuke Moriyama ◽  
Rie Wakabayashi ◽  
Masahiro Goto ◽  
Noriho Kamiya
Keyword(s):  
Cell Culture ◽  
Horseradish Peroxidase ◽  
Ethylene Glycol ◽  
Cell Sheet ◽  
Viable Cell ◽  
Poly Ethylene Glycol ◽  
Redox Responsive ◽  
Poly Ethylene ◽  
Responsive Hydrogels

Horseradish peroxidase-mediated preparation of redox-responsive hydrogels composed of thiolated poly(ethylene glycol) and gelatin allowed the proliferation of cells on the gel and the harvest as a viable cell sheet.

Best of both worlds: Diels–Alder chemistry towards fabrication of redox-responsive degradable hydrogels for protein release

RSC Advances ◽  
2016 ◽  
Vol 6 (78) ◽  
pp. 74757-74764 ◽  
Author(s):  
Ismail Altinbasak ◽  
Rana Sanyal ◽  
Amitav Sanyal
Keyword(s):  
Ethylene Glycol ◽  
Alder Reaction ◽  
Diels Alder ◽  
Protein Release ◽  
Poly Ethylene Glycol ◽  
Diels Alder Reaction ◽  
Redox Responsive ◽  
Poly Ethylene ◽  
Responsive Hydrogels

Poly(ethylene glycol)-based redox-responsive hydrogels have been preparedviathe Diels–Alder reaction between a furan-containing hydrophilic copolymer and a disulfide-containing bis-maleimide based crosslinker.

Enzymatic preparation of a redox-responsive hydrogel for encapsulating and releasing living cells

2014 ◽  
Vol 50 (44) ◽  
pp. 5895-5898 ◽  
Author(s):  
Kousuke Moriyama ◽  
Kosuke Minamihata ◽  
Rie Wakabayashi ◽  
Masahiro Goto ◽  
Noriho Kamiya
Keyword(s):  
Horseradish Peroxidase ◽  
Ethylene Glycol ◽  
Mammalian Cells ◽  
Living Cells ◽  
Cross Linking ◽  
Enzymatic Preparation ◽  
Poly Ethylene Glycol ◽  
Redox Responsive ◽  
Poly Ethylene

Horseradish peroxidase-mediated oxidative cross-linking of a thiolated poly(ethylene glycol) allows the preparation of a hydrogel that can encapsulate and release living mammalian cells.

Therapeutic effects of boronate ester cross-linked injectable hydrogels for the treatment of hepatocellular carcinoma

2021 ◽  
Author(s):  
Jae Min Jung ◽  
Seong Han Kim ◽  
V. H. Giang Phan ◽  
Thavasyappan Thambi ◽  
Doo Sung Lee
Keyword(s):  
Hepatocellular Carcinoma ◽  
Ethylene Glycol ◽  
Therapeutic Effects ◽  
Poly Ethylene Glycol ◽  
Injectable Hydrogels ◽  
High Incidence ◽  
High Incidence Rate ◽  
The Common ◽  
Poly Ethylene ◽  
Responsive Hydrogels

Hepatocellular carcinoma is the common malignant with a high incidence rate and responsible for the highest cause of cancer-related deaths. Herein, we developed a thermo-responsive hydrogels comprised of poly(ε-caprolactone-co-lactide)-b-poly(ethylene glycol)-b-poly(ε-caprolactone-co-lactide...

Tailorable Cell Culture Platforms from Enzymatically Cross-Linked Multifunctional Poly(ethylene glycol)-Based Hydrogels

2013 ◽  
Vol 14 (2) ◽  
pp. 413-423 ◽  
Author(s):  
Donna J. Menzies ◽  
Andrew Cameron ◽  
Trent Munro ◽  
Ernst Wolvetang ◽  
Lisbeth Grøndahl ◽  
...  
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals A Non-Cytotoxic Resin for Micro-Stereolithography for Cell Cultures of HUVECs

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 246 ◽  
Author(s):  
Max Männel ◽  
Carolin Fischer ◽  
Julian Thiele
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
Umbilical Vein ◽  
Polymer Materials ◽  
Poly Ethylene Glycol ◽  
Glycol Diacrylate ◽  
3D Printed ◽  
Poly Ethylene ◽  
The Impact

Three-dimensional (3D) printing of microfluidic devices continuously replaces conventional fabrication methods. A versatile tool for achieving microscopic feature sizes and short process times is micro-stereolithography (µSL). However, common resins for µSL lack biocompatibility and are cytotoxic. This work focuses on developing new photo-curable resins as a basis for µSL fabrication of polymer materials and surfaces for cell culture. Different acrylate- and methacrylate-based compositions are screened for material characteristics including wettability, surface roughness, and swelling behavior. For further understanding, the impact of photo-absorber and photo-initiator on the cytotoxicity of 3D-printed substrates is studied. Cell culture experiments with human umbilical vein endothelial cells (HUVECs) in standard polystyrene vessels are compared to 3D-printed parts made from our library of homemade resins. Among these, after optimizing material composition and post-processing, we identify selected mixtures of poly(ethylene glycol) diacrylate (PEGDA) and poly(ethylene glycol) methyl ethyl methacrylate (PEGMEMA) as most suitable to allow for fabricating cell culture platforms that retain both the viability and proliferation of HUVECs. Next, our PEGDA/PEGMEMA resins will be further optimized regarding minimal feature size and cell adhesion to fabricate microscopic (microfluidic) cell culture platforms, e.g., for studying vascularization of HUVECs in vitro.

Enzymatically crosslinked hydrogels based on linear poly(ethylene glycol) polymer: performance and mechanism

2017 ◽  
Vol 8 (45) ◽  
pp. 7017-7024 ◽  
Author(s):  
Kaixuan Ren ◽  
Bin Li ◽  
Qinghua Xu ◽  
Chunsheng Xiao ◽  
Chaoliang He ◽  
...  
Keyword(s):  
Horseradish Peroxidase ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Linear Poly ◽  
Poly Ethylene ◽  
Performance And Mechanism

A horseradish peroxidase-catalyzed hydrogel based on a double-end tyramine conjugated linear poly(ethylene glycol) polymer is developed and clarified.

Redox-responsive organometallic hydrogels for in situ metal nanoparticle synthesis

2015 ◽  
Vol 51 (4) ◽  
pp. 636-639 ◽  
Author(s):  
B. Zoetebier ◽  
M. A. Hempenius ◽  
G. J. Vancso
Keyword(s):  
Ethylene Glycol ◽  
Nanoparticle Synthesis ◽  
Metal Nanoparticle ◽  
Poly Ethylene Glycol ◽  
Redox Responsive ◽  
Poly Ethylene

A hydrogel composed of poly(ferrocenylsilane) polyanion and poly(ethylene glycol) chains was used to form relatively well-defined, unaggregated Pd(0) nanoparticles (8.2 ± 2.2 nm) from K2PdCl4 salts.

Triple Redox Responsive Poly(Ethylene Glycol)-Polycaprolactone Polymeric Nanocarriers for Fine-Controlled Drug Release

2016 ◽  
Vol 17 (4) ◽  
pp. 1600295 ◽  
Author(s):  
Caiyan Zhao ◽  
Leihou Shao ◽  
Jianqing Lu ◽  
Chenying Zhao ◽  
Yujie Wei ◽  
...  
Keyword(s):  
Drug Release ◽  
Ethylene Glycol ◽  
Controlled Drug Release ◽  
Poly Ethylene Glycol ◽  
Polymeric Nanocarriers ◽  
Redox Responsive ◽  
Poly Ethylene
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