Casein glycomacropeptide hydrolysate exerts cytoprotection against H2O2-induced oxidative stress in RAW 264.7 macrophages via ROS-dependent heme oxygenase-1 expression

RSC Advances ◽  
2015 ◽  
Vol 5 (6) ◽  
pp. 4511-4523 ◽  
Author(s):  
Xue Cheng ◽  
Dong-Xiao Gao ◽  
Jia-Jia Song ◽  
Fa-Zheng Ren ◽  
Xue-Ying Mao
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages ◽  
Protective Actions

Casein glycomacropeptide hydrolysate had antioxidant activity and exerted protective actions against H2O2-induced oxidative stress via induction of Nrf2-mediated HO-1 expression in RAW 264.7 macrophages.

scholarly journals Protective Effect of Glutathione against Oxidative Stress-induced Cytotoxicity in RAW 264.7 Macrophages through Activating the Nuclear Factor Erythroid 2-Related Factor-2/Heme Oxygenase-1 Pathway

Antioxidants ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 82 ◽  
Author(s):  
Da Kwon ◽  
Hee-Jae Cha ◽  
Hyesook Lee ◽  
Su-Hyun Hong ◽  
Cheol Park ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Protective Effect ◽  
Heme Oxygenase ◽  
Raw 264.7 Cells ◽  
Heme Oxygenase 1 ◽  
Raw 264.7 ◽  
Related Factor ◽  
Nuclear Factor ◽  
Raw 264.7 Macrophages

Reactive oxygen species (ROS), products of oxidative stress, contribute to the initiation and progression of the pathogenesis of various diseases. Glutathione is a major antioxidant that can help prevent the process through the removal of ROS. The aim of this study was to evaluate the protective effect of glutathione on ROS-mediated DNA damage and apoptosis caused by hydrogen peroxide, H2O2, in RAW 264.7 macrophages and to investigate the role of the nuclear factor erythroid 2-related factor-2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway. The results showed that the decrease in the survival rate of RAW 264.7 cells treated with H2O2 was due to the induction of DNA damage and apoptosis accompanied by the increased production of ROS. However, H2O2-induced cytotoxicity and ROS generation were significantly reversed by glutathione. In addition, the H2O2-induced loss of mitochondrial membrane potential was related to a decrease in adenosine triphosphate (ATP) levels, and these changes were also significantly attenuated in the presence of glutathione. These protective actions were accompanied by a increase in the expression rate of B-cell lymphoma-2 (Bcl-2)/Bcl-2-associated X protein (Bax) and poly(ADP-ribose) polymerase cleavage by the inactivation of caspase-3. Moreover, glutathione-mediated cytoprotective properties were associated with an increased activation of Nrf2 and expression of HO-1; however, the inhibition of the HO-1 function using an HO-1 specific inhibitor, zinc protoporphyrin IX, significantly weakened the cytoprotective effects of glutathione. Collectively, the results demonstrate that the exogenous administration of glutathione is able to protect RAW 264.7 cells against oxidative stress-induced mitochondria-mediated apoptosis along with the activity of the Nrf2/HO-1 signaling pathway.

scholarly journals Heme oxygenase-1 mediates the anti-inflammatory effect of molecular hydrogen in LPS-stimulated RAW 264.7 macrophages

2013 ◽  
Vol 11 (10) ◽  
pp. 1060-1066 ◽  
Author(s):  
Hong-Guang Chen ◽  
Ke-Liang Xie ◽  
Huan-Zhi Han ◽  
Wei-Na Wang ◽  
Da-Quan Liu ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Molecular Hydrogen ◽  
Heme Oxygenase 1 ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Sesamin increases heme oxygenase-1 protein in RAW 264.7 macrophages through inhibiting its ubiquitination process

2014 ◽  
Vol 741 ◽  
pp. 214-221 ◽  
Author(s):  
Mizuki Fukunaga ◽  
Masatoshi Ohnishi ◽  
Ayano Shiratsuchi ◽  
Takuya Kawakami ◽  
Madoka Takahashi ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages
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