scholarly journals DNA sequencing by synthesis using 3′-O-azidomethyl nucleotide reversible terminators and surface-enhanced Raman spectroscopic detection

RSC Advances ◽  
2014 ◽  
Vol 4 (90) ◽  
pp. 49342-49346 ◽  
Author(s):  
Mirkó Palla ◽  
Wenjing Guo ◽  
Shundi Shi ◽  
Zengmin Li ◽  
Jian Wu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Dna Sequencing ◽  
Surface Enhanced Raman Spectroscopy ◽  
Raman Shift ◽  
Azido Group ◽  
Raman Spectroscopic ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sequencing By Synthesis

DNA sequencing using surface enhanced Raman spectroscopy for the detection of the unique Raman shift of the azido group was demonstrated using 3′-O-azidomethyl nucleotide reversible terminators in polymerase reaction.

Mapping gold nanoparticles on and in edible leaves in situ using surface enhanced Raman spectroscopy

RSC Advances ◽  
2016 ◽  
Vol 6 (65) ◽  
pp. 60152-60159 ◽  
Author(s):  
Zhiyun Zhang ◽  
Huiyuan Guo ◽  
Yingqing Deng ◽  
Baoshan Xing ◽  
Lili He
Keyword(s):  
Gold Nanoparticles ◽  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Mapping Technique ◽  
Raman Spectroscopic ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Spinach Leaves

A surface enhanced Raman spectroscopic (SERS) mapping technique was applied to qualitatively detect and characterize gold nanoparticles on and in spinach leaves in situ.

Gold nanosponges (AuNS): a versatile nanostructure for surface-enhanced Raman spectroscopic detection of small molecules and biomolecules

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7278-7282 ◽  
Author(s):  
Gregory Q. Wallace ◽  
Mariachiara S. Zuin ◽  
Mohammadali Tabatabaei ◽  
Pierangelo Gobbo ◽  
François Lagugné-Labarthet ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Small Molecules ◽  
Surface Enhanced Raman Spectroscopy ◽  
Raman Spectroscopic ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Prepared by simple pour and mix chemistry, gold nanosponges (AuNS) are versatile structures for surface-enhanced Raman spectroscopy (SERS).

scholarly journals Detection and Aggregation of Listeria Monocytogenes Using Polyclonal Antibody Gold-Coated Magnetic Nanoshells Surface-Enhanced Raman Spectroscopy Substrates

2021 ◽  
Vol 3 ◽  
Author(s):  
Robert T. Busch ◽  
Farzia Karim ◽  
Yvonne Sun ◽  
H. Christopher Fry ◽  
Yuzi Liu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Listeria Monocytogenes ◽  
Polyclonal Antibody ◽  
Surface Enhanced Raman Spectroscopy ◽  
Raman Shift ◽  
Label Free ◽  
Surface Binding ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Induced Aggregation

Magnetic nanoshells with tailored surface chemistry can enhance bacterial detection and separation technologies. This work demonstrated a simple technique to detect, capture, and aggregate bacteria with the aid of end-functionalized polyclonal antibody gold-coated magnetic nanoshells (pAb-Lis-AuMNs) as surface-enhanced Raman spectroscopy (SERS) probes. Listeria monocytogenes were used as the pathogenic bacteria and the pAb-Lis-AuMNs, 300 nm diameter, were used as probes allowing facile magnetic separation and aggregation. An optimized covalent bioconjugation procedure between the magnetic nanoshells and the polyclonal antibody was performed at pH six via a carbodiimide crosslinking reaction. Spectroscopic and morphological characterization techniques confirmed the fabrication of stable pAb-Lis-AuMNs. The resulting pAb-Lis-AuMNs acted as a SERS probe for L. monocytogenes based on the targeted capture via surface binding interactions and magnetically induced aggregation. Label-free SERS measurements were recorded for the minimum detectable amount of L. monocytogenes based on the SERS intensity at the 1388 cm−1 Raman shift. L. monocytogenes concentrations exhibited detection limits in the range of 104–107 CFU ml−1, before and after aggregation. By fitting these concentrations, the limit of detection of this method was ∼103 CFU ml−1. Using a low-intensity magnetic field of 35 G, pAb-Lis-AuMNs aggregated L. monocytogenes as demonstrated with microscopy techniques, including SEM and optical microscopy. Overall, this work presents a label-free SERS probe method comprised of a surface-modified polyclonal antibody sub-micron magnetic nanoshell structures with high sensitivity and magnetic induced separation that could lead to the fabrication of multiple single-step sensors.

Synthesis of corrugated surface AgNWs and their applications in surface enhanced Raman spectroscopy

CrystEngComm ◽  
2020 ◽  
Vol 22 (12) ◽  
pp. 2183-2196
Author(s):  
Shah Fahad ◽  
Haojie Yu ◽  
Li Wang ◽  
Yang Wang ◽  
Tarig Elshaarani ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Electromagnetic Field ◽  
Electrical Properties ◽  
Surface Enhanced Raman Spectroscopy ◽  
Raman Spectroscopic ◽  
Corrugated Surface ◽  
Field Generation ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Sensing

Among metals, AgNWs are considered to be excellent materials for use in surface enhancement Raman spectroscopic (SERS) sensing due to their superior electrical properties, strong electromagnetic field generation and strong enhancement intensity.

scholarly journals Surface-enhanced Raman spectroscopic identification in fingerprints based on adhesive Au nanofilm

RSC Advances ◽  
2018 ◽  
Vol 8 (43) ◽  
pp. 24477-24484 ◽  
Author(s):  
Jieru Lin ◽  
Chenjie Zhang ◽  
Minmin Xu ◽  
Yaxian Yuan ◽  
Jianlin Yao
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Raman Spectroscopic ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Spectroscopic Identification

A facial approach was developed successfully for transferring and recovering the pattern of fingerprints based on surface enhanced Raman spectroscopy and adhesive Au nanofilm.

Surface-enhanced Raman spectroscopy (SERS) nanoprobes for ratiometric detection of cancer cells

2019 ◽  
Vol 7 (5) ◽  
pp. 815-822 ◽  
Author(s):  
Linhu Li ◽  
Mengling Liao ◽  
Yingfan Chen ◽  
Beibei Shan ◽  
Ming Li
Keyword(s):  
Raman Spectroscopy ◽  
Cancer Cells ◽  
Surface Enhanced Raman Spectroscopy ◽  
Expression Ratio ◽  
Raman Spectroscopic ◽  
Ratiometric Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

A surface-enhanced Raman spectroscopic strategy is developed for ratiometric detection of cancer cells by quantifying the expression ratio of extracellular biomarkers.

scholarly journals Evaluation of Surface Enhanced Raman Spectroscopy (SERS) for Detection of Acetone in the Context of Food Safety and Quality Application

2012 ◽  
Vol 1 (1) ◽  
pp. 3 ◽  
Author(s):  
Punyatoya Mohapatra ◽  
Suranjan Panigrahi
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Raman Shift ◽  
Sers Substrate ◽  
Experimental Conditions ◽  
Sensing System ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Contaminated Food ◽  
Food Safety And Quality

<p class="Body">Development of sensors for detection of contaminated food products is important to ensure safety of the community. This paper focuses on detection of a compound, acetone associated with pathogen contamination and spoilage of beef in packaged condition. Surface Enhanced Raman Spectroscopy (SERS) technique was used to develop an integrated sensing system. This sensing system utilized laboratory prepared silver sol as the SERS substrate to detect acetone at low ppm and high ppb concentration. A set of eight experiments with two different experimental conditions were conducted. The first four set provided an estimated lower detection limit (LDL) ranging between 69.63 ppm and 630.17 ppm corresponding to the 1711 cm<sup>-1</sup> and 532 cm<sup>-1</sup> Raman shift (cm<sup>-1</sup>) regions, respectively. The second four sets of experiments provided a lower estimated LDL ranging between 9.78 ppm and 43.45 ppm corresponding to 1222 cm<sup>-1</sup> and 532 cm<sup>-1</sup>, respectively. This study demonstrated the ability of this sensing system to detect the indicator compound.</p>

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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