A new strategy for fluorometric detection of ascorbic acid based on hydrolysis and redox reaction

RSC Advances ◽  
2014 ◽  
Vol 4 (66) ◽  
pp. 35112 ◽  
Author(s):  
Yirong Zhao ◽  
Yinhui Li ◽  
Yijun Wang ◽  
Jing Zheng ◽  
Ronghua Yang
2021 ◽  
Vol 41 ◽  
pp. 108-120
Author(s):  
M Gallorini ◽  
◽  
R Di Carlo ◽  
S Pilato ◽  
A Ricci ◽  
...  

Human dental pulp stem cell (DPSC) differentiation toward the osteoblastic phenotype is enhanced when culture media are supplemented with differentiating factors, i.e. ascorbic acid, β-glycerophosphate and dexamethasone. Liposomes, spherical vesicles formed by a phospholipid bilayer, are frequently used as carriers for drugs, growth factors and hydrophobic molecules. The aim of this work was to speed up DPSC commitment to the osteogenic lineage by embedding differentiating factors within liposomes. Firstly, liposomes were prepared by rehydrating a phospholipidic thin film and characterised in terms of dimensions. Secondly, liposome-exposed DPSCs were characterised by their immunophenotypic profile. Levels of CD90 were significantly decreased in the presence of liposomes filled with ascorbic acid, β-glycerophosphate and dexamethasone (Lipo-Mix) with respect to normal differentiation medium (DM), while CD73 and CD29 expression were enhanced, suggesting osteogenic commitment. Additionally, an appreciable extracellular matrix deposition is detected. Thirdly, the Lipo-Mix formulation better increases alkaline phosphatase activity and levels of Collagen I secretion with respect to DM. In parallel, the new liposome formulation is capable of decreasing the release of H2O2 and of triggering a precocious antioxidant cell response, redressing the redox balance required upon mesenchymal stem cell commitment to osteogenesis. It can be therefore hypothesised that Lipo-Mix could represent a suitable tool for clinical regenerative purposes in the field of tissue engineering by speeding up DPSC osteogenic commitment, mineralised matrix deposition and remodelling.


2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Dong Liu ◽  
Yang Liu ◽  
Haoqing Hou ◽  
Tianyan You

A novel sonochemical process, using hydrogen peroxide in a laboratory ultrasonic bath, was employed to pretreat the carbon nanofiber (CNF) for creating oxygen-rich groups on the surface of CNF. After the sonochemical process, the CNF showed good hydrophilicity and high electrochemical activity. Compared to normal pretreatment process, this sonochemical process is timesaving and effective for dispersion and functionalization of CNF. The resulting CNF showed high catalytic activity toward the oxidation of DA. A carbon paste electrode modified by CNF (CPE-CNF) was used to determine the dopamine (DA) in the presence of ascorbic acid (AA). The detection limit is 0.05 μM, with the linear range from 0.05 μM to 6.4 μM.


2018 ◽  
Vol 42 (9) ◽  
pp. 6707-6712 ◽  
Author(s):  
Dan Zhang ◽  
Na Li ◽  
Yiming Ma ◽  
Ruixue Wang ◽  
Jinyi Wang ◽  
...  

Three chemosensors with azide groups at different sites were designed for rapid detection of ascorbic acid in garlic slice tissues.


2013 ◽  
Vol 61 (2) ◽  
pp. 161-166 ◽  
Author(s):  
Farhana Haque ◽  
MS Rahman ◽  
Etmina Ahmed ◽  
PK Bakshi ◽  
AA Shaikh

The electrochemical redox behavior of Cu(II), and Cu(II) in presence of ascorbic acid has been investigated at glassy carbon electrode (GCE) in aqueous phosphate buffer solution (PBS) at various pH. In the studied pH range the voltammogram consists of a cathodic and an anodic peak. Cu(II) follows one step two-electron transfer electrochemical redox reaction. The intensities of both cathodic and anodic peak current increase with increasing scan rate that are consistent with Randles-Sevcik equation. The linear behavior of peak current with the square root of scan rate indicates that the electrochemical processes are diffusion controlled. In presence of ascorbic acid both of the cathodic and anodic peak current of Cu(II) is reduced and peak positions are shifted compared to those of Cu(II) alone. These observation suggest that Cu(II) undergoes a strong interaction with ascorbic acid in the studied medium. DOI: http://dx.doi.org/10.3329/dujs.v61i2.17064 Dhaka Univ. J. Sci. 61(2): 161-166, 2013 (July)


2019 ◽  
Vol 21 (29) ◽  
pp. 16180-16189 ◽  
Author(s):  
Ségolène Palussière ◽  
Jérémy Cure ◽  
Andréa Nicollet ◽  
Pierre Fau ◽  
Katia Fajerwerg ◽  
...  

We report on a new strategy to synthesize Al/CuO nanothermites from commercial Al and ultra-small chemically synthesized CuO nanoparticles coated with alkylamine ligands.


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