A simple label-free rhodamine 6G SERS probe for quantitative analysis of trace As3+in an aptamer–nanosol

RSC Advances ◽  
2014 ◽  
Vol 4 (62) ◽  
pp. 32960 ◽  
Author(s):  
Lingling Ye ◽  
Guiqing Wen ◽  
Jinchao Dong ◽  
Yanghe Luo ◽  
Qingye Liu ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Rhodamine 6G ◽  
Label Free

scholarly journals Label-free Quantitative Analysis of Changes in Broiler Liver Proteins under Heat Stress using SWATH-MS Technology

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Xiangfang Tang ◽  
Qingshi Meng ◽  
Jie Gao ◽  
Sheng Zhang ◽  
Hongfu Zhang ◽  
...  
Keyword(s):  
Heat Stress ◽  
Quantitative Analysis ◽  
Label Free

scholarly journals Electrochemical detection of different p53 conformations by using nanostructured surfaces

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sarah Tonello ◽  
Francesca Stradolini ◽  
Giulia Abate ◽  
Daniela Uberti ◽  
Mauro Serpelloni ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Limit Of Detection ◽  
Direct Electrochemistry ◽  
Protein Quantification ◽  
Label Free ◽  
Limited Information ◽  
Clinical Practices ◽  
Nanostructured Surfaces ◽  
Integrated Devices ◽  
Biochemical Assays

AbstractProtein electrochemistry represents a powerful technique for investigating the function and structure of proteins. Currently available biochemical assays provide limited information related to the conformational state of proteins and high costs. This work provides novel insights into the electrochemical investigation of the metalloprotein p53 and its redox products using label-free direct electrochemistry and label-based antibody-specific approaches. First, the redox activities of different p53 redox products were qualitatively investigated on carbon-based electrodes. Then, focusing on the open p53 isoform (denatured p53), a quantitative analysis was performed, comparing the performances of different bulk and nanostructured materials (carbon and platinum). Overall, four different p53 products could be successfully discriminated, from wild type to denatured. Label-free analysis suggested a single electron exchange with electron transfer rate constants on the order of 1 s−1. Label-based analysis showed decreasing affinity of pAb240 towards denatured, oxidized and nitrated p53. Furthermore, platinum nanostructured electrodes showed the highest enhancement of the limit of detection in the quantitative analysis (100 ng/ml). Overall, the obtained results represent a first step towards the implementation of highly requested complex integrated devices for clinical practices, with the aim to go beyond simple protein quantification.

Comparison of One- and Two-dimensional Liquid Chromatography Approaches in the Label-free Quantitative Analysis ofMethylocella silvestris

2012 ◽  
Vol 11 (9) ◽  
pp. 4755-4763 ◽  
Author(s):  
Nisha A. Patel ◽  
Andrew Crombie ◽  
Susan E. Slade ◽  
Konstantinos Thalassinos ◽  
Chris Hughes ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Two Dimensional ◽  
Label Free

scholarly journals Quantitative Analysis of the Seminal Plasma Proteome in Secondary Hypogonadism

2019 ◽  
Vol 8 (12) ◽  
pp. 2128 ◽  
Author(s):  
Giuseppe Grande ◽  
Federica Vincenzoni ◽  
Francesca Mancini ◽  
Ferran Barrachina ◽  
Antonella Giampietro ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Seminal Plasma ◽  
Control Group ◽  
Testosterone Replacement Therapy ◽  
Male Hypogonadism ◽  
Grey Zone ◽  
Label Free ◽  
Clinical Markers ◽  
Before And After ◽  
Secondary Hypogonadism

In the grey zone of testosterone levels between 8 and 12 nmol/L, the usefulness of therapy is controversial; as such, markers of tissue action of androgens may be helpful in adjusting clinical decisions. To better understand the effect of the hypothalamic-pituitary-testicular axis on male accessory secretion, we performed a proteomic quantitative analysis of seminal plasma in patients with secondary hypogonadism, before and after testosterone replacement therapy (TRT). Ten male patients with postsurgical hypogonadotrophic hypogonadism were enrolled in this study, and five of these patients were evaluated after testosterone treatment. Ten men with proven fertility were selected as a control group. An aliquot of seminal plasma from each individual was subjected to an in-solution digestion protocol and analyzed using an Ultimate 3000 RSLC-nano HPLC apparatus coupled to a LTQ Orbitrap Elite mass spectrometer. The label-free quantitative analysis was performed via Precursor Ions Area Detector Node. Eleven proteins were identified as decreased in hypogonadic patients versus controls, which are primarily included in hydrolase activity and protein binding activity. The comparison of the proteome before and after TRT comes about within the discovery of six increased proteins. This is the primary application of quantitative proteomics pointed to uncover a cluster of proteins reflecting an impairment not only of spermatogenesis but of the epididymal and prostate epithelial cell secretory function in male hypogonadism. The identified proteins might represent putative clinical markers valuable within the follow-up of patients with distinctive grades of male hypogonadism.

scholarly journals msCompare: A Framework for Quantitative Analysis of Label-free LC-MS Data for Comparative Candidate Biomarker Studies

2012 ◽  
Vol 11 (6) ◽  
pp. M111.015974 ◽  
Author(s):  
Berend Hoekman ◽  
Rainer Breitling ◽  
Frank Suits ◽  
Rainer Bischoff ◽  
Peter Horvatovich
Keyword(s):  
Quantitative Analysis ◽  
Label Free

Label-Free Aptamer Fluorescence Determination of Trace Pb2+ Using AuPd Nanoalloy Probe as Catalyst

2013 ◽  
Vol 631-632 ◽  
pp. 18-21 ◽  
Author(s):  
Zhi Liang Jiang ◽  
Mei Ling Tang ◽  
Qing Ye Liu ◽  
Ai Hui Liang
Keyword(s):  
Detection Limit ◽  
Fluorescence Quenching ◽  
Fluorescence Intensity ◽  
Water Samples ◽  
Catalytic Effect ◽  
Rhodamine 6G ◽  
Label Free ◽  
Double Stranded Dna ◽  
Fluorescence Determination

In the condition of 1.24 mmol/L EDTANa2, 16.7 mmol/L NaCl and 0.17 mmol/L Tris, the substrate chain of double-stranded DNA (dsDNA) could be cracked by Pb2+ to release single-stranded DNA (ssDNA) that adsorb onto AuPd nanoparticle (AuPdNP) and form stable AuPdNP-ssDNA, but the dsDNA can not protect AuPdNP that were aggregated to big AuPdNP aggregations (AuPdNPA) under the action of NaCl. The AuPdNP-ssDNA and AuPdNPA could be separated by centrifugation. With the concentration of Pb2+ increased, the released ssDNA increased, the AuPdNP-ssDNA in centrifugation solution increased and the catalytic effect enhanced on the fluorescence quenching reaction of Rhodamine 6G (Rh6G) and NaH2PO2, which led the fluorescence intensity at 552nm to decrease. The decreased fluorescence intensity (ΔF552nm) was linear to the concentration of Pb2+ in the range of 0.33-8.00 nmol/L, a detection limit of 0.21 nmol/L. The proposed method was applied to detect Pb2+ in water samples, with satisfactory results.

Label-free quantitative analysis for studying the interactions between nanoparticles and plasma proteins

2012 ◽  
Vol 405 (2-3) ◽  
pp. 635-645 ◽  
Author(s):  
Anna Laura Capriotti ◽  
Giulio Caracciolo ◽  
Giuseppe Caruso ◽  
Chiara Cavaliere ◽  
Daniela Pozzi ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Plasma Proteins ◽  
Label Free
Sign in / Sign up

Export Citation Format

Share Document