Erythrocyte deformation in a microfluidic cross-slot channel

RSC Advances ◽  
2014 ◽  
Vol 4 (68) ◽  
pp. 36079 ◽  
Author(s):  
Yann Henon ◽  
Gregory J. Sheard ◽  
Andreas Fouras
Keyword(s):  
Slot Channel ◽  
Erythrocyte Deformation

Flow dynamics and heat transfer in a rotating slot channel

1978 ◽  
Vol 35 (1) ◽  
pp. 823-827 ◽  
Author(s):  
O. N. Ovchinnikov ◽  
E. M. Smirnov
Keyword(s):  
Heat Transfer ◽  
Flow Dynamics ◽  
Slot Channel

scholarly journals Erythrocyte deformation in shear flow: influences of internal viscosity, membrane stiffness, and hematocrit

Blood ◽  
1987 ◽  
Vol 69 (3) ◽  
pp. 727-734 ◽  
Author(s):  
K Kon ◽  
N Maeda ◽  
T Shiga
Keyword(s):  
Viscoelastic Properties ◽  
Shear Force ◽  
Flow Lines ◽  
Extracellular Medium ◽  
Intact Cells ◽  
Suspension Viscosity ◽  
Applied Shear Stress ◽  
Erythrocyte Deformation ◽  
Membrane Stiffness ◽  
Internal Viscosity

The effect of shear force (depending on shear rate and viscosity of extracellular medium) and hematocrit of RBC suspension on RBC deformation was studied quantitatively using a cone-plate rheoscope with various kinds of cells, ie, partially hemolyzed (PH) cells, density-fractionated intact cells, and diamide-treated cells. The deformation index (DI) of ellipsoidally deformed cells was shown to be a function of beta gamma eta ex(eta ex/eta in)alpha, where gamma eta ex is applied shear stress, eta ex and eta in are external and internal viscosities, respectively, and alpha and beta are adjustable parameters related to the membrane viscoelastic properties. The increase of suspension viscosity at higher hematocrits (Hts) generally enhanced the ellipsoidal deformation of cells, in the same manner as increasing the suspending medium viscosity of a diluted cell suspension. The suppressing effect on cell deformation appeared above a certain Ht. When intact cells were mixed with glutaraldehyde-treated, hardened cells, the ellipsoidal deformation of intact cells was disturbed. The suppression of deformation probably occurred through disturbance of laminar flow-lines around intact cells.

Participation of cAMP in a signal-transduction pathway relating erythrocyte deformation to ATP release

2001 ◽  
Vol 281 (4) ◽  
pp. C1158-C1164 ◽  
Author(s):  
Randy S. Sprague ◽  
Mary L. Ellsworth ◽  
Alan H. Stephenson ◽  
Andrew J. Lonigro
Keyword(s):  
Signal Transduction ◽  
Adenylyl Cyclase ◽  
Atp Release ◽  
Signal Transduction Pathway ◽  
Intracellular Camp ◽  
Transmembrane Conductance Regulator ◽  
Transduction Pathway ◽  
Camp Analog ◽  
Dependent Protein ◽  
Erythrocyte Deformation

Previously, we reported that red blood cells (RBCs) of rabbits and humans release ATP in response to mechanical deformation and that this release of ATP requires the activity of the cystic fibrosis transmembrane conductance regulator (CFTR). It was reported that cAMP, acting through a cAMP-dependent protein kinase, PKA, is an activator of CFTR. Here we investigate the hypothesis that cAMP stimulates ATP release from RBCs. Incubation of human and rabbit RBCs with the direct activator of adenylyl cyclase, forskolin (10 or 100 μM), with IBMX (100 μM), resulted in ATP release and increases in intracellular cAMP. In addition, epinephrine (1 μM), a receptor-mediated activator of adenylyl cyclase, stimulated ATP release from rabbit RBCs. Moreover, incubation of human and rabbit RBCs with an active cAMP analog [adenosine 3′5′-cyclic monophosphorothioate Sp-isomer (Sp-cAMP, 100 μM)] resulted in ATP release. In contrast, forskolin and Sp-cAMP were without effect on dog RBCs, cells known not to release ATP in response to deformation. When rabbit RBCs were incubated with the inactive cAMP analog and inhibitor of PKA activity, adenosine 3′,5′-cyclic monophosphorothioate Rp-isomer (100 μM), deformation-induced ATP release was attenuated. These results are consistent with the hypothesis that adenylyl cyclase and cAMP are components of a signal-transduction pathway relating RBC deformation to ATP release from human and rabbit RBCs.

Erythrocyte Deformation in Human Muscular Dystrophy

Science ◽  
1974 ◽  
Vol 184 (4133) ◽  
pp. 165-166 ◽  
Author(s):  
D. W. Matheson ◽  
J. L. Howland
Keyword(s):  
Muscular Dystrophy ◽  
Erythrocyte Deformation

Laser diffractometry of erythrocyte deformation under hypo-osmotic hemolysis

2000 ◽  
Author(s):  
Stanislav S. Bessmeltsev ◽  
Alexander V. Lendiaev ◽  
Yulia A. Skvortsova ◽  
Vladimir A. Tarlykov
Keyword(s):  
Osmotic Hemolysis ◽  
Laser Diffractometry ◽  
Erythrocyte Deformation

Kinetics of phase transformations on isothermal slot channel walls

1987 ◽  
Vol 52 (1) ◽  
pp. 58-63
Author(s):  
P. A. Novikov ◽  
L. Ya. Lyubin ◽  
V. I. Novikova
Keyword(s):  
Phase Transformations ◽  
Slot Channel ◽  
Kinetics Of
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