Enzymatic synthesis of amylose nanocomposite microbeads using amylosucrase from Deinococcus geothermalis

RSC Advances ◽  
2014 ◽  
Vol 4 (50) ◽  
pp. 26421-26424 ◽  
Author(s):  
Min-Cheol Lim ◽  
Dong-Ho Seo ◽  
Ji-Hoon Jung ◽  
Cheon-Seok Park ◽  
Young-Rok Kim

This communication reports a biological approach to synthesize pure amylose microbeads and amylose–SWCNT composite microbeads using the amylosucrase from Deinococcus geothermalis.

2009 ◽  
Vol 344 (13) ◽  
pp. 1612-1619 ◽  
Author(s):  
Jong-Hyun Jung ◽  
Dong-Ho Seo ◽  
Suk-Jin Ha ◽  
Myoung-Chong Song ◽  
Jaeho Cha ◽  
...  

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0207466 ◽  
Author(s):  
Se-Won Jang ◽  
Chi Heung Cho ◽  
Young-Sung Jung ◽  
Chansu Rha ◽  
Tae-Gyu Nam ◽  
...  

Synlett ◽  
1991 ◽  
Vol 1991 (04) ◽  
pp. 310-312
Author(s):  
Patrizia Ferraboschi ◽  
Daria Brembilla ◽  
Paride Grisenti ◽  
Enzo Santaniello

2012 ◽  
Vol 3 (2) ◽  
pp. 177-179
Author(s):  
Dr. K.Vinoth Kumar Dr. K.Vinoth Kumar ◽  
◽  
Dr. C. Udayasoorian Dr. C. Udayasoorian

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Nicole Danielle Osier ◽  
George M Garrity

2018 ◽  
Author(s):  
Andrea Pérez-Villa ◽  
Thomas Georgelin ◽  
Jean-François Lambert ◽  
Marie-Christine Maurel ◽  
François Guyot ◽  
...  

Understanding the mechanism of spontaneous formation of ribonucleotides under realistic prebiotic conditions is a key open issue of origins-of-life research. In cells, <i>de novo</i> and salvage nucleotide enzymatic synthesis combines 5-phospho-α -D-ribose-1-diphosphate ( α-PRPP) and nucleobases. Interestingly, these reactants are also known as prebiotically plausible compounds. Combining ab initio simulations with mass spectrometry experiments, we compellingly demonstrate that nucleobases and α -PRPP spontaneously combine, through the same facile mechanism, forming both purine and pyrimidine ribonucleotides, under mild hydrothermal conditions. Surprisingly, this mechanism is very similar to the biological one, and yields ribonucleotides with the same anomeric carbon chirality as in biological systems. These results suggest that natural selection might have optimized – through enzymes – a pre-existing ribonucleotide formation mechanism, carrying it forward to modern life forms.


2017 ◽  
Author(s):  
Andrea Pérez-Villa ◽  
Thomas Georgelin ◽  
Jean-François Lambert ◽  
Marie-Christine Maurel ◽  
François Guyot ◽  
...  

Understanding the mechanism of spontaneous formation of ribonucleotides under realistic prebiotic conditions is a key open issue of origins-of-life research. In cells, <i>de novo</i> and salvage nucleotide enzymatic synthesis combines 5-phospho-α -D-ribose-1-diphosphate ( α-PRPP) and nucleobases. Interestingly, these reactants are also known as prebiotically plausible compounds. Combining ab initio simulations with mass spectrometry experiments, we compellingly demonstrate that nucleobases and α -PRPP spontaneously combine, through the same facile mechanism, forming both purine and pyrimidine ribonucleotides, under mild hydrothermal conditions. Surprisingly, this mechanism is very similar to the biological one, and yields ribonucleotides with the same anomeric carbon chirality as in biological systems. These results suggest that natural selection might have optimized – through enzymes – a pre-existing ribonucleotide formation mechanism, carrying it forward to modern life forms.


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