Nomenclature Abstract for Deinococcus geothermalis Ferreira et al. 1997.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Nicole Danielle Osier ◽  
George M Garrity
Keyword(s):  
Deinococcus Geothermalis

scholarly journals Synthesis of Hyperbranched Glycoconjugates by the Combined Action of Potato Phosphorylase and Glycogen Branching Enzyme from Deinococcus geothermalis

Polymers ◽  
2012 ◽  
Vol 4 (1) ◽  
pp. 674-690 ◽  
Author(s):  
Jeroen van der Vlist ◽  
Martin Faber ◽  
Lizette Loen ◽  
Teunis J. Dijkman ◽  
Lia A. T. W. Asri ◽  
...  
Keyword(s):  
Branching Enzyme ◽  
Deinococcus Geothermalis ◽  
Combined Action ◽  
Glycogen Branching Enzyme

scholarly journals Transposition of Insertion Sequences was Triggered by Oxidative Stress in Radiation-Resistant Bacterium Deinococcus geothermalis

2019 ◽  
Vol 7 (10) ◽  
pp. 446 ◽  
Author(s):  
Chanjae Lee ◽  
Nakjun Choi ◽  
Min K. Bae ◽  
Kyungsil Choo ◽  
Sung-Jae Lee
Keyword(s):  
Oxidative Stress ◽  
Family Member ◽  
Mutant Strain ◽  
Mutant Cell ◽  
Carotenoid Biosynthesis ◽  
Wild Type ◽  
Is Element ◽  
Deinococcus Geothermalis ◽  
Key Enzyme ◽  
Radiation Resistant

During an oxidative stress-response assay on a putative Dps-like gene-disrupted Δdgeo_0257 mutant strain of radiation-resistant bacterium Deinococcus geothermalis, a non-pigmented colony was observed among the normal reddish color colonies. This non-pigmented mutant cell subsequently displayed higher sensitivity to H2O2. While carotenoid has a role in protecting as scavenger of reactive oxygen species the reddish wild-type strain from radiation and oxidative stresses, it is hypothesized that the carotenoid biosynthesis pathway has been disrupted in the mutant D. geothermalis cell. Here, we show that, in the non-pigmented mutant cell of interest, phytoene desaturase (Dgeo_0524, crtI), a key enzyme in carotenoid biosynthesis, was interrupted by transposition of an ISDge7 family member insertion sequence (IS) element. RNA-Seq analysis between wild-type and Δdgeo_0257 mutant strains revealed that the expression level of ISDge5 family transposases, but not ISDge7 family members, were substantially up-regulated in the Δdgeo_0257 mutant strain. We revealed that the non-pigmented strain resulted from the genomic integration of ISDge7 family member IS elements, which were also highly up-regulated, particularly following oxidative stress. The transposition path for both transposases is a replicative mode. When exposed to oxidative stress in the absence of the putative DNA binding protein Dgeo_0257, a reddish D. geothermalis strain became non-pigmented. This transformation was facilitated by transposition of an ISDge7 family IS element into a gene encoding a key enzyme of carotenoid biosynthesis. Further, we present evidence of additional active transposition by the ISDge5 family IS elements, a gene that was up-regulated during the stationary phase regardless of the presence of oxidative stress.

Heterologous expression of Deinococcus geothermalis amylosucrase in Corynebacterium glutamicum for luteolin glucoside production

2020 ◽  
Vol 135 ◽  
pp. 109505
Author(s):  
Young-Wook Chin ◽  
Se-Won Jang ◽  
Hee-Soon Shin ◽  
Tae-Wan Kim ◽  
Sun-Ki Kim ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Corynebacterium Glutamicum ◽  
Deinococcus Geothermalis

Redox potential change by the cystine importer affected on enzymatic antioxidant protection in Deinococcus geothermalis

2020 ◽  
Vol 113 (6) ◽  
pp. 779-790
Author(s):  
Kyungsil Choo ◽  
Minwook Kim ◽  
Sama Abdi Nansa ◽  
Min K. Bae ◽  
Chanjae Lee ◽  
...  
Keyword(s):  
Redox Potential ◽  
Potential Change ◽  
Antioxidant Protection ◽  
Enzymatic Antioxidant ◽  
Deinococcus Geothermalis

scholarly journals Engineering Deinococcus geothermalis for Bioremediation of High-Temperature Radioactive Waste Environments

2003 ◽  
Vol 69 (8) ◽  
pp. 4575-4582 ◽  
Author(s):  
Hassan Brim ◽  
Amudhan Venkateswaran ◽  
Heather M. Kostandarithes ◽  
James K. Fredrickson ◽  
Michael J. Daly
Keyword(s):  
High Temperature ◽  
Radioactive Waste ◽  
Elevated Temperatures ◽  
Deinococcus Radiodurans ◽  
Nitrilotriacetic Acid ◽  
Thermophilic Bacterium ◽  
Prospective Development ◽  
Deinococcus Geothermalis ◽  
Radiation Resistant

ABSTRACT Deinococcus geothermalis is an extremely radiation-resistant thermophilic bacterium closely related to the mesophile Deinococcus radiodurans, which is being engineered for in situ bioremediation of radioactive wastes. We report that D. geothermalis is transformable with plasmids designed for D. radiodurans and have generated a Hg(II)-resistant D. geothermalis strain capable of reducing Hg(II) at elevated temperatures and in the presence of 50 Gy/h. Additionally, D. geothermalis is capable of reducing Fe(III)-nitrilotriacetic acid, U(VI), and Cr(VI). These characteristics support the prospective development of this thermophilic radiophile for bioremediation of radioactive mixed waste environments with temperatures as high as 55°C.

Biosynthesis of (+)-catechin glycosides using recombinant amylosucrase from Deinococcus geothermalis DSM 11300

2011 ◽  
Vol 49 (2) ◽  
pp. 246-253 ◽  
Author(s):  
Hyun-Kug Cho ◽  
Hee-Hang Kim ◽  
Dong-Ho Seo ◽  
Jong-Hyun Jung ◽  
Ji-Hae Park ◽  
...  
Keyword(s):  
Deinococcus Geothermalis

scholarly journals An Antioxidant Defense System in Radiation-Resistant Bacterium Deinococcus geothermalis against Oxidative Stress

2021 ◽  
Author(s):  
Chanjae Lee ◽  
Min K. Bae ◽  
Sung-Jae Lee
Keyword(s):  
Oxidative Stress ◽  
Dna Binding ◽  
Carotenoid Biosynthesis ◽  
Antioxidant Defense System ◽  
Redox Balance ◽  
Primary Response ◽  
Resistant Bacterium ◽  
Loss Of Function ◽  
Deinococcus Geothermalis ◽  
Radiation Resistant

A radiation-resistant bacterium, Deinococcus geothermalis has various stress response mechanisms, including antioxidation. Features that maintain vitality at high radiation doses include the following: enzymatic scavengers of ROS such as catalase, SOD, and peroxidase; strain-specific DNA repair systems such as Deinococcal unique proteins; non-enzymatic responses such as manganese complexes, carotenoids, and DNA-binding proteins. This chapter summarizes the primary response mechanism by redox balance centered on the cystine transporter. It also reviews action characteristics of DNA-binding protein Dps and a putative LysR family protein, and effects on loss of function of the carotenoid biosynthesis genes by transposition of insertion sequences. Environmental adaptation and molecular evolution of radiation-resistant bacterium are also considered to explain the potentials of molecular behavior induced by oxidative stress.

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