scholarly journals Highly selective and sensitive detection of glutathione using mesoporous silica nanoparticles capped with disulfide-containing oligo(ethylene glycol) chains

2015 ◽  
Vol 13 (4) ◽  
pp. 1017-1021 ◽  
Author(s):  
Sameh El Sayed ◽  
Cristina Giménez ◽  
Elena Aznar ◽  
Ramón Martínez-Máñez ◽  
Félix Sancenón ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Ethylene Glycol ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Sensitive Detection ◽  
Fluorimetric Detection ◽  
Safranin O

Mesoporous silica nanoparticles loaded with safranin O and capped with disulfide-containing oligo(ethylene glycol) chains were used for the selective and sensitive fluorimetric detection of GSH.

scholarly journals Hybrid Mesoporous Silica Nanoparticles Grafted with 2-(tert-butylamino)ethyl Methacrylate-b-poly(ethylene Glycol) Methyl Ether Methacrylate Diblock Brushes as Drug Nanocarrier

Molecules ◽  
2020 ◽  
Vol 25 (1) ◽  
pp. 195 ◽  
Author(s):  
Abdullah M Alswieleh ◽  
Abeer M Beagan ◽  
Bayan M Alsheheri ◽  
Khalid M Alotaibi ◽  
Mansour D Alharthi ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Ethylene Glycol ◽  
Silica Nanoparticles ◽  
Methyl Ether ◽  
Diblock Copolymer ◽  
Mesoporous Silica Nanoparticles ◽  
Poly Ethylene Glycol ◽  
Ethyl Methacrylate ◽  
Poly Ethylene ◽  
Glycol Methyl Ether

This paper introduces the synthesis of well-defined 2-(tert-butylamino)ethyl methacrylate-b-poly(ethylene glycol) methyl ether methacrylate diblock copolymer, which has been grafted onto mesoporous silica nanoparticles (PTBAEMA-b-PEGMEMA-MSNs) via atom transfer radical polymerization (ATRP). The ATRP initiators were first attached to the MSN surfaces, followed by the ATRP of 2-(tert-butylamino)ethyl methacrylate (PTBAEMA). CuBr2/bipy and ascorbic acid were employed as the catalyst and reducing agent, respectively, to grow a second polymer, poly(ethylene glycol) methyl ether methacrylate (PEGMEMA). The surface structures of these fabricated nanomaterials were then analyzed using Fourier Transform Infrared (FTIR) spectroscopy. The results of Thermogravimetric Analysis (TGA) show that ATRP could provide a high surface grafting density for polymers. Dynamic Light Scattering (DLS) was conducted to investigate the pH-responsive behavior of the diblock copolymer chains on the nanoparticle surface. In addition, multifunctional pH-sensitive PTBAEMA-b-PEGMEMA-MSNs were loaded with doxycycline (Doxy) to study their capacities and long-circulation time.

scholarly journals Intra-articular Delivery of Celastrol by Hollow Mesoporous Silica Nanoparticles for pH-sensitive Anti-inflammatory Therapy against Knee Osteoarthritis

2020 ◽  
Author(s):  
Tian Jin ◽  
Di Wu ◽  
Xiao-Ming Liu ◽  
Jiang-Tao Xu ◽  
Bing-Jie Ma ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Knee Osteoarthritis ◽  
Silica Nanoparticles ◽  
Therapeutic Effect ◽  
Mesoporous Silica Nanoparticles ◽  
Ph Responsive ◽  
Fast Green ◽  
Poor Solubility ◽  
Hollow Mesoporous Silica ◽  
Safranin O

Abstract Celastrol has been proven effective in anti-inflammatory but was limited in the clinic due to the poor solubility and side effects induced by low bioavailability. Osteoarthritis has acidic and inflammatory environment. Our aim was to load celastrol into HMSNs and capped with chitosan to construct a pH-responsive nanoparticle medicine(CSL@HMSNs-Cs), which is of high solubility for osteoarthritis intra-articular injection treatment. Methods: The CSL@HMSNs-Cs were assembled and the characteristics were measured. The CSL@HMSNs-Cs was applied in vitro in the chondrocytes collected from rats cartilage tissue and in vivo in the MIA induced knee osteoarthritis rats via intra-articular injection. Cytotoxicity assay, pH-responsive release, pain behavior, MRI, safranin o fast green staining, ELISA and western blot analysis were applied to evaluate the bioavailability and therapeutic effect of [email protected]: CSL@HMSNs-Cs was stable due to the protection of the chitosan layers in alkaline environment(pH=7.7) but revealed good solubility and therapeutic effect in acidic environment(pH=6.0). The cytotoxicity assay showed no cytotoxicity at relatively low concentration(200 μg/mL) and the cell viability of chondrocytes stimulated by IL-1β was increased in CSL@HMSNs-Cs group. Paw withdrawal threshold in CSL@HMSNs-Cs group is increased, and MRI and Safranin O Fast Green staining showed improvements in articular surface erosion and joint effusion. The upregulated expression levels of IL-1β, TNF-α, IL-6, MMP-3 and MMP-13 and NF-κB signaling pathway of chondrocytes were inhibited in CSL@HMSNs-Cs group.Conclusion: Hollow mesoporous silica nanoparticles were an ideal carrier for natural drugs with poor solubility and were of high biocompatibility for intra-articular injection. These intra-articular injectable CSL@HMSNs-Cs with improved solubility, present a pH-responsive therapeutic strategy against osteoarthritis.

scholarly journals Poly(oligo(ethylene glycol) methyl ether methacrylate) Capped pH-Responsive Poly(2-(diethylamino)ethyl methacrylate) Brushes Grafted on Mesoporous Silica Nanoparticles as Nanocarrier

Polymers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 823
Author(s):  
Khalid M. Alotaibi ◽  
Abdurrahman A. Almethen ◽  
Abeer M. Beagan ◽  
Latifah H. Alfhaid ◽  
Maqusood Ahamed ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Ethylene Glycol ◽  
Silica Nanoparticles ◽  
Methyl Ether ◽  
Mesoporous Silica Nanoparticles ◽  
Cancer Drug ◽  
Ph Responsive ◽  
Ethyl Methacrylate ◽  
Anti Cancer ◽  
Glycol Methyl Ether

In this paper, a new pH-responsive nanosystem based on mesoporous silica nanoparticles (MSNs) was developed for cancer therapy. Poly(2-(diethylamino) ethyl methacrylate) (PDEAEMA) was grafted on their outer surface and acts as a gatekeeper, followed by subsequent modification of the polymer by cysteine (MSN-PDEAEMA-Cys) and poly(oligo(ethylene glycol) methyl ether methacrylate) (MSN-PDEAEMA-Cys-POEGMEMA). The physicochemical properties of these nanocarriers were characterized using scanning and transmission electron microscopies (SEM and TEM), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and dynamic light scattering (DLS). The synthesized nanoparticles were well-dispersed with a diameter of ca. 200 nm. The obtained XPS results confirm the successful modification of MSN-PDEAEMA with Cys and POEGMEMA by increasing the peak intensity of C–O and C=O groups at 286.5 and 288.5 eV, respectively. An anti-cancer drug, doxorubicin (DOX), was encapsulated into the fabricated nanoplatform. The DOX release amount at physiological pH of 7.4 was limited (10%), while an accumulation drug release of ca. 35% was accomplished after 30 h in acidic media. The MTT cell line was used to assess the cytotoxicity of the unloaded and DOX-loaded fabricated nanoplatforms. Upon loading of DOX on these nanomaterials, they showed significant toxicity to human liver cancer cells. These results suggest that the prepared nano-structured materials showed good biocompatibility as well, and they can serve as nanocarriers for the delivery of anti-cancer drugs.

scholarly journals Intra-articular Delivery of Celastrol by Hollow Mesoporous Silica Nanoparticles for pH-sensitive Anti-inflammatory Therapy against Knee Osteoarthritis

2020 ◽  
Author(s):  
Tian Jin ◽  
Di Wu ◽  
Xiao-Ming Liu ◽  
Jiang-Tao Xu ◽  
Bing-Jie Ma ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Knee Osteoarthritis ◽  
Silica Nanoparticles ◽  
Therapeutic Effect ◽  
Mesoporous Silica Nanoparticles ◽  
Ph Responsive ◽  
Fast Green ◽  
Poor Solubility ◽  
Hollow Mesoporous Silica ◽  
Safranin O

Abstract Celastrol, like other natural drugs, has proven effective in anti-inflammatory but was limited in clinic because of the poor solubility and side effects induced by low bioavailability. In recent years, hollow mesoporous silica nanoparticles (HMSNs) have received extensive attention in biomedical field due to their outstanding biocompatibility and multiple modification. Osteoarthritis has acidic and inflammatory environment. In this study, celastrol was loaded in HMSNs and capped with chitosan to construct a pH-responsive nanoparticle medicine(CSL@HMSNs-Cs) with high solubility for osteoarthritis intra-articular injection treatment. Methods: The CSL@HMSNs-Cs were assembled and the characteristics were measured. The CSL@HMSNs-Cs was applied in vitro in the chondrocytes collected from rats cartilage tissue and in vivo in the MIA induced knee osteoarthritis rats via intra-articular injection. Cytotoxicity assay, pH-responsive release, pain behavior, MRI, safranin o fast green staining, ELISA and western blot analysis were applied to evaluate the bioavailability and therapeutic effect of CSL@HMSNs-Cs. Results: CSL@HMSNs-Cs was stable due to the protection of the chitosan layers in alkaline environment(pH=7.7) but revealed good solubility and therapeutic effect in acidic environment(pH=6.0). The cytotoxicity assay showed no cytotoxicity at relatively low concentration(200 μg/mL) and the cell viability of chondrocytes stimulated by IL-1β was increased in CSL@HMSNs-Cs group. Paw withdrawal threshold in CSL@HMSNs-Cs group is increased, and MRI and Safranin O Fast Green staining showed improvements in articular surface erosion and joint effusion. The upregulated expression levels of IL-1β, TNF-α, IL-6, MMP-3 and MMP-13 and NF-κB signaling pathway of chondrocytes were inhibited in CSL@HMSNs-Cs group. Conclusion: Hollow mesoporous silica nanoparticles were idea carrier for natural drug with poor solubility and were of high biocompatibility for intra-articular injection. These intra-articular injectable CSL@HMSNs-Cs with improved solubility present a pH-responsive therapeutic strategy against osteoarthritis.

Anti-CD155 and anti-CD112 monoclonal antibodies conjugated to a fluorescent mesoporous silica nanosensor encapsulating rhodamine 6G and fluorescein for sensitive detection of liver cancer cells

The Analyst ◽  
2016 ◽  
Vol 141 (16) ◽  
pp. 4933-4940 ◽  
Author(s):  
Liang Tao ◽  
Chaojun Song ◽  
Chenyang Huo ◽  
Yuanjie Sun ◽  
Chunmei Zhang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Liver Cancer ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Cancer Cells ◽  
Rhodamine 6G ◽  
Mesoporous Silica Nanoparticles ◽  
Sensitive Detection ◽  
Liver Cancer Cells

Sensitive detection of liver cancer cells using anti-CD155 and anti-CD112 monoclonal antibodies conjugated to ultrabright fluorescent mesoporous silica nanoparticles (FMSNs) encapsulating Rhodamine 6G and fluorescein was developed.

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