Bacterial adhesion force quantification by fluidic force microscopy

Nanoscale ◽  
2015 ◽  
Vol 7 (9) ◽  
pp. 4070-4079 ◽  
Author(s):  
Eva Potthoff ◽  
Dario Ossola ◽  
Tomaso Zambelli ◽  
Julia A. Vorholt
Keyword(s):  
Single Cell ◽  
Bacterial Adhesion ◽  
Adhesion Force ◽  
State Of The Art ◽  
Force Spectroscopy ◽  
Force Microscopy ◽  
Cell Force ◽  
Serial Measurements ◽  
Cell Fixation

Fluidic force microscopy demonstrates the potential to quantify bacterial adhesion by single-cell force spectroscopy, achieving higher immobilization forces than state-of-the-art cell-cantilever interactions. Reversible cell fixation on the tip allows for serial measurements of many cells in the nN range using a single cantilever.

Quantifying bacterial adhesion on antifouling polymer brushes via single-cell force spectroscopy

2015 ◽  
Vol 6 (31) ◽  
pp. 5740-5751 ◽  
Author(s):  
Cesar Rodriguez-Emmenegger ◽  
Sébastien Janel ◽  
Andres de los Santos Pereira ◽  
Michael Bruns ◽  
Frank Lafont
Keyword(s):  
Atomic Force Microscope ◽  
Single Cell ◽  
Bacterial Adhesion ◽  
Bacterial Cell ◽  
Polymer Brushes ◽  
Force Spectroscopy ◽  
Adhesion Forces ◽  
Atomic Force ◽  
Cell Force

The adhesion forces between a single bacterial cell and different polymer brushes were measured directly with an atomic force microscope and correlated with their resistance to fouling.

scholarly journals Cell Adhesion on Dynamic Supramolecular Surfaces Probed by Fluid Force Microscopy-Based Single-Cell Force Spectroscopy

ACS Nano ◽  
2017 ◽  
Vol 11 (4) ◽  
pp. 3867-3874 ◽  
Author(s):  
Shrikrishnan Sankaran ◽  
Leena Jaatinen ◽  
Jenny Brinkmann ◽  
Tomaso Zambelli ◽  
Janos Vörös ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Force Spectroscopy ◽  
Fluid Force ◽  
Force Microscopy ◽  
Cell Force

scholarly journals Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e112582 ◽  
Author(s):  
Laura Andolfi ◽  
Eugenia Bourkoula ◽  
Elisa Migliorini ◽  
Anita Palma ◽  
Anja Pucer ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Single Cell ◽  
Glioma Cells ◽  
Force Spectroscopy ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cell Force

scholarly journals Effect of Graphene Oxide Packing on Bacterial Adhesion using Single Cell Force Spectroscopy

2018 ◽  
Vol 114 (3) ◽  
pp. 352a-353a
Author(s):  
Elise Linna ◽  
Sara BinAhmed ◽  
Benjamin L. Stottrup ◽  
Santiago Romero-Vargas Castrill¢n
Keyword(s):  
Graphene Oxide ◽  
Single Cell ◽  
Bacterial Adhesion ◽  
Force Spectroscopy ◽  
Cell Force

scholarly journals Assessing the Functional Properties of TiZr Nanotubular Structures for Biomedical Applications, through Nano-Scratch Tests and Adhesion Force Maps

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 900
Author(s):  
Maria Vardaki ◽  
Aida Pantazi ◽  
Ioana Demetrescu ◽  
Marius Enachescu
Keyword(s):  
Surface Roughness ◽  
Functional Properties ◽  
Bacterial Adhesion ◽  
Biomedical Applications ◽  
Adhesion Force ◽  
Roughness Parameters ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Mean ◽  
Scratch Tests

In this work we present the results of a functional properties assessment via Atomic Force Microscopy (AFM)-based surface morphology, surface roughness, nano-scratch tests and adhesion force maps of TiZr-based nanotubular structures. The nanostructures have been electrochemically prepared in a glycerin + 15 vol.% H2O + 0.2 M NH4F electrolyte. The AFM topography images confirmed the successful preparation of the nanotubular coatings. The Root Mean Square (RMS) and average (Ra) roughness parameters increased after anodizing, while the mean adhesion force value decreased. The prepared nanocoatings exhibited a smaller mean scratch hardness value compared to the un-coated TiZr. However, the mean hardness (H) values of the coatings highlight their potential in having reliable mechanical resistances, which along with the significant increase of the surface roughness parameters, which could help in improving the osseointegration, and also with the important decrease of the mean adhesion force, which could lead to a reduction in bacterial adhesion, are providing the nanostructures with a great potential to be used as a better alternative for Ti implants in dentistry.

Sign in / Sign up

Export Citation Format

Share Document