scholarly journals Cell Adhesion on Dynamic Supramolecular Surfaces Probed by Fluid Force Microscopy-Based Single-Cell Force Spectroscopy

ACS Nano ◽  
2017 ◽  
Vol 11 (4) ◽  
pp. 3867-3874 ◽  
Author(s):  
Shrikrishnan Sankaran ◽  
Leena Jaatinen ◽  
Jenny Brinkmann ◽  
Tomaso Zambelli ◽  
Janos Vörös ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Force Spectroscopy ◽  
Fluid Force ◽  
Force Microscopy ◽  
Cell Force

scholarly journals Quantifying the effect of electric current on cell adhesion studied by single-cell force spectroscopy

2016 ◽  
Vol 11 (1) ◽  
pp. 011004 ◽  
Author(s):  
Leena Jaatinen ◽  
Eleanore Young ◽  
Jari Hyttinen ◽  
János Vörös ◽  
Tomaso Zambelli ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Electric Current ◽  
Force Spectroscopy ◽  
Cell Force

Stimulated single-cell force spectroscopy to quantify cell adhesion receptor crosstalk

PROTEOMICS ◽  
2010 ◽  
Vol 10 (7) ◽  
pp. 1455-1462 ◽  
Author(s):  
Jens Friedrichs ◽  
Jonne Helenius ◽  
Daniel J. Müller
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Force Spectroscopy ◽  
Adhesion Receptor ◽  
Receptor Crosstalk ◽  
Cell Force ◽  
Cell Adhesion Receptor

scholarly journals Quantitative investigation of calcimimetic R568 on beta cell adhesion and mechanics using AFM single-cell force spectroscopy

FEBS Letters ◽  
2014 ◽  
Vol 588 (7) ◽  
pp. 1178-1183 ◽  
Author(s):  
Eleftherios Siamantouras ◽  
Claire E. Hills ◽  
Mustafa Y.G. Younis ◽  
Paul E. Squires ◽  
Kuo-Kang Liu
Keyword(s):  
Cell Adhesion ◽  
Beta Cell ◽  
Single Cell ◽  
Force Spectroscopy ◽  
Quantitative Investigation ◽  
Cell Force

scholarly journals Atomic Force Microscopy Demonstrates that Candida glabrata Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces

mSphere ◽  
2019 ◽  
Vol 4 (3) ◽  
Author(s):  
Claire Valotteau ◽  
Valeria Prystopiuk ◽  
Brendan P. Cormack ◽  
Yves F. Dufrêne
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Candida Glabrata ◽  
Hydrophobic Interactions ◽  
Antifungal Drugs ◽  
Content Type ◽  
Force Microscopy ◽  
Abiotic Surfaces ◽  
Cell Force ◽  
Broad Role

ABSTRACT The fungal pathogen Candida glabrata can cause both mucosal and disseminated infections. Cell adhesion, a key step in colonization and infection, depends in C. glabrata primarily on the Epa family of cell adhesion proteins. While Epa proteins have been documented to mediate specific adhesion to host glycans, some of them also promote nonspecific adhesion to abiotic surfaces, though this is incompletely understood. Here we address this issue using a combination of genetics and single-cell force measurements. By quantifying the forces driving the attachment of single C. glabrata cells to hydrophobic and hydrophilic substrates, we show that cell adhesion is strongly increased by loss of Sir-mediated silencing. Using a series of mutant strains lacking specific EPA genes, we demonstrate unexpectedly that three major Epa proteins, Epa1, Epa6, and Epa7, primarily contribute to both hydrophilic and hydrophobic interactions, suggesting a broad role for the Epa adhesins in mediating specific and nonspecific adherence and implicating Epa genes in biofilm formation on abiotic surfaces. IMPORTANCE Candida glabrata cell wall proteins mediate the attachment of C. glabrata to abiotic surfaces through molecular interactions that are poorly understood. Here, we study the forces engaged in Epa-dependent adhesion using single-cell techniques. Fungal adhesion to hydrophilic and hydrophobic substrates involves mainly three Epa proteins, suggesting a broad role for the Epa adhesins in mediating adherence. These proteins might represent a potential target for the development of innovative antifungal drugs.

In Situ Quantification of Living Cell Adhesion Forces: Single Cell Force Spectroscopy with a Nanotweezer

Langmuir ◽  
2014 ◽  
Vol 30 (10) ◽  
pp. 2952-2959 ◽  
Author(s):  
Hui Xie ◽  
Munan Yin ◽  
Weibin Rong ◽  
Lining Sun
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Living Cell ◽  
Force Spectroscopy ◽  
Adhesion Forces ◽  
Cell Force

scholarly journals Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e112582 ◽  
Author(s):  
Laura Andolfi ◽  
Eugenia Bourkoula ◽  
Elisa Migliorini ◽  
Anita Palma ◽  
Anja Pucer ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Single Cell ◽  
Glioma Cells ◽  
Force Spectroscopy ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cell Force

scholarly journals 2B4 Engagement Mediates Rapid LFA-1 and Actin-Dependent NK Cell Adhesion to Tumor Cells as Measured by Single Cell Force Spectroscopy

2011 ◽  
Vol 186 (5) ◽  
pp. 2757-2764 ◽  
Author(s):  
Sabrina C. Hoffmann ◽  
André Cohnen ◽  
Thomas Ludwig ◽  
Carsten Watzl
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Tumor Cells ◽  
Nk Cell ◽  
Force Spectroscopy ◽  
Cell Force

scholarly journals Contributions of Galectin-3 and -9 to Epithelial Cell Adhesion Analyzed by Single Cell Force Spectroscopy

2007 ◽  
Vol 282 (40) ◽  
pp. 29375-29383 ◽  
Author(s):  
Jens Friedrichs ◽  
Juha M. Torkko ◽  
Jonne Helenius ◽  
Terhi P. Teräväinen ◽  
Joachim Füllekrug ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cell ◽  
Single Cell ◽  
Force Spectroscopy ◽  
Galectin 3 ◽  
Cell Force
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