Strontium eluting graphene hybrid nanoparticles augment osteogenesis in a 3D tissue scaffold

Nanoscale ◽  
2015 ◽  
Vol 7 (5) ◽  
pp. 2023-2033 ◽  
Author(s):  
Sachin Kumar ◽  
Kaushik Chatterjee
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Metallic Nanoparticles ◽  
Hybrid Nanoparticles ◽  
Graphene Sheets ◽  
Polymer Scaffolds ◽  
Tissue Scaffold

Hybrid nanoparticles of graphene sheets decorated with strontium metallic nanoparticles were incorporated in polymer scaffolds and the advantages in bone tissue engineering are demonstrated.

scholarly journals Reconstruction of Bone Defect Based on the Fusion of Osteoblasts and MSM/HA/PLGA Porous Scaffolds

2020 ◽  
Author(s):  
weiling huo ◽  
Xiaodong Wu ◽  
Yancheng zheng ◽  
Jian Cheng ◽  
Qiang Xu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Defect ◽  
Drug Loading ◽  
Release Time ◽  
Porous Scaffolds ◽  
Polymer Scaffolds ◽  
Experimental Basis

Reconstruction of bone defect is one of the difficult problems in orthopedic treatment, and bone tissue scaffold implantation is the most promising direction of bone defect reconstruction. In this study, we used the combination of HA (Hydroxyapatite) and PLGA [Poly (lactic-co-glycolic acid)] in the construction of polymer scaffolds, and introduced bioactive MSM (Methyl sulfonyl methane) into polymer scaffolds to prepare porous scaffolds. The osteoblasts, isolated and cultured in vitro, were seeded in the porous scaffolds to construct tissue-engineered scaffolds. Meanwhile, the model of rabbit radius defect was constructed to evaluate the biological aspects of five tissue-engineered scaffolds, which provided experimental basis for the application of the porous scaffolds in bone tissue engineering. The SEM characterization showed the pore size of porous scaffolds was uniform and the porosity was about 90%. The results of contact Angle testing suggested that the hydrophobic porous scaffold surface could effectively promote cell adhesion and cell proliferation, while mechanical property test showed good machinability. The results of drug loading and release efficiency of MSM showed that porous scaffolds could load MSM efficiently and prolong the release time of MSM. In vitro incubation of porous scaffolds and osteoblasts showed that the addition of a small quantity of MSM could promote the infiltration and proliferation of osteoblasts on the porous scaffolds. Similar results were obtained by implanting the tissue-engineered scaffolds, fused with the osteoblasts and MSM/HA/PLGA porous scaffolds, into the rabbit radius defect, which provided experimental basis for the application of the MSM/HA/PLGA porous scaffolds in bone tissue engineering.

Porosity and swelling properties of novel polyurethane–ascorbic acid scaffolds prepared by different procedures for potential use in bone tissue engineering

2016 ◽  
Vol 49 (5) ◽  
pp. 440-456 ◽  
Author(s):  
J Kucinska-Lipka ◽  
M Marzec ◽  
I Gubanska ◽  
H Janik
Keyword(s):  
Tissue Engineering ◽  
Ascorbic Acid ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Saline Water ◽  
Hexamethylene Diisocyanate ◽  
Swelling Properties ◽  
Tissue Scaffold ◽  
Salt Particle ◽  
Water Swelling

In this work, a novel polyurethane (PU) system based on poly(ethylene-butylene) adipate diol, 1,6-hexamethylene diisocyanate, 1,4-butanediol, and ascorbic acid was used to prepare scaffolds with potential applications in bone tissue engineering. Two fabrication methods to obtain porous materials were chosen: phase separation (PS)/salt particle leaching (PL) and solvent casting (SC)/salt PL. The calculated porosity demonstrated that scaffolds with a higher porosity were obtained (76–86%) using the PS/PL method. The morphology of pores was examined with the use of optical stereomicroscope and scanning electron microscope. The appropriate porosity, pore morphology, and swelling properties for bone tissue scaffolds were obtained for our novel PU system by the PS/PL method using 15 wt% solution of PU in the mixture of dimethylformamide (DMF)/tetrahydrofuran solvents and by SC/PL method from 20 wt% from DMF. The distilled water and saline water swelling for those samples was also appropriate for bone tissue scaffold application.

New fabrication methods of bioactive and biodegradable scaffolds for bone tissue engineering

2011 ◽  
Vol 47 (3) ◽  
pp. 261-270 ◽  
Author(s):  
Youngmee Jung ◽  
Su Hee Kim ◽  
Sang-Heon Kim ◽  
Soo Hyun Kim
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Polymer ◽  
Cellular Interaction ◽  
Solvent Casting ◽  
Polymer Scaffolds ◽  
Casting Method ◽  
Biodegradable Scaffolds ◽  
Sintering Method

Bioceramic and polymers have been used as matrices for bone tissue engineering, and successful bone regeneration depends on cellular interaction with these matrices. The aim of this study was to fabricate polymer/ceramics composites with a novel sintering method. Also, we prepared homogenous porous poly(lactide-co-glycolide (PLGA) scaffolds in the supercritical CO2. These scaffolds had homogenous porous structure and high tensile and compressive mechanical properties compared to the scaffold prepared by conventional solvent casting method. This study revealed that generating bioactive and porous polymer scaffolds with novel sintering method or supercritical fluid technique could be useful for bone tissue engineering.

scholarly journals 3D Hierarchical, Nanostructured Chitosan/PLA/HA Scaffolds Doped with TiO2/Au/Pt NPs with Tunable Properties for Guided Bone Tissue Engineering

Polymers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 792 ◽  
Author(s):  
Julia Radwan-Pragłowska ◽  
Łukasz Janus ◽  
Marek Piątkowski ◽  
Dariusz Bogdał ◽  
Dalibor Matysek
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Metallic Nanoparticles ◽  
Three Dimensional ◽  
Poly Lactic Acid ◽  
Promising Tool ◽  
Surgical Treatments ◽  
Autologous Grafts ◽  
Inorganic Components

Bone tissue is the second tissue to be replaced. Annually, over four million surgical treatments are performed. Tissue engineering constitutes an alternative to autologous grafts. Its application requires three-dimensional scaffolds, which mimic human body environment. Bone tissue has a highly organized structure and contains mostly inorganic components. The scaffolds of the latest generation should not only be biocompatible but also promote osteoconduction. Poly (lactic acid) nanofibers are commonly used for this purpose; however, they lack bioactivity and do not provide good cell adhesion. Chitosan is a commonly used biopolymer which positively affects osteoblasts’ behavior. The aim of this article was to prepare novel hybrid 3D scaffolds containing nanohydroxyapatite capable of cell-response stimulation. The matrixes were successfully obtained by PLA electrospinning and microwave-assisted chitosan crosslinking, followed by doping with three types of metallic nanoparticles (Au, Pt, and TiO2). The products and semi-components were characterized over their physicochemical properties, such as chemical structure, crystallinity, and swelling degree. Nanoparticles’ and ready biomaterials’ morphologies were investigated by SEM and TEM methods. Finally, the scaffolds were studied over bioactivity on MG-63 and effect on current-stimulated biomineralization. Obtained results confirmed preparation of tunable biomimicking matrixes which may be used as a promising tool for bone-tissue engineering.

Hydrophobicity as a design criterion for polymer scaffolds in bone tissue engineering

Biomaterials ◽  
2005 ◽  
Vol 26 (21) ◽  
pp. 4423-4431 ◽  
Author(s):  
Edwin J.P. Jansen ◽  
Raymond E.J. Sladek ◽  
Hila Bahar ◽  
Avinoam Yaffe ◽  
Marion J. Gijbels ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Design Criterion ◽  
Polymer Scaffolds
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