Reduced graphene oxide growth on 316L stainless steel for medical applications

Nanoscale ◽  
2014 ◽  
Vol 6 (15) ◽  
pp. 8664-8670 ◽  
Author(s):  
L. Cardenas ◽  
J. MacLeod ◽  
J. Lipton-Duffin ◽  
D. G. Seifu ◽  
F. Popescu ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Graphene Oxide ◽  
Cell Viability ◽  
Reduced Graphene Oxide ◽  
Mammalian Cells ◽  
316L Stainless Steel ◽  
Medical Applications ◽  
Oxide Growth ◽  
Stainless Steel 316L ◽  
Reduced Graphene

We describe a new method for the growth of reduced graphene oxide rGO on the 316L alloy of stainless steel 316L. Cell viability studies reveal that these rGO coatings do not have toxic effects on mammalian cells.

Reduced Graphene Oxide Paper: Fabrication by a Green Thermal Reduction Method and Preliminary Study of its In Vitro Cytotoxicity

2017 ◽  
Vol 45 ◽  
pp. 199-207 ◽  
Author(s):  
Xin Wang ◽  
Peng Li ◽  
Claudia Luedecke ◽  
Qiang Zhang ◽  
Zan Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Mammalian Cells ◽  
Reduction Method ◽  
Heating Temperature ◽  
Synthesis Method ◽  
Thermal Reduction ◽  
Graphene Films ◽  
Reduced Graphene

Graphene films have been intensively explored because of their unique mechanical and physicochemical properties for potential applications in field of tissue engineering and implants. However, for biomedical applications, it is necessary to fully understand the toxicity and biocompatibility of the prepared graphene films since different synthesis method might lead to different biological properties. Here we report a step-by-step thermal reduction method of preparing reduced graphene oxide (rGO) film directly on various substrates at low heating temperature (below about 200 °C) without requiring any chemical reduction agent like hydrazine or other reductants (therefore we call it green method). Slowly heating GO hydrosol that was coated on the surface of a glass cell-culture dish or inside of a polypropylene tube from room temperature to 60, 100, and 160 °C for 12 h, respectively, a shiny and flat surface without crumpled structure or tiny pores was formed. We peeled it off from the substrate to explore its cytotoxicity. The results exhibited that the rGO film was biocompatible with Cal-72 cell but against Escherichia coli bacteria. Our work confirmed that rGO film produced by the green reduction method is cytocompatible with mammalian cells, which makes this rGO film a promising material for tissue engineering scaffold or as a surface-modification coating of an implant.

scholarly journals Metal-Carbon Interactions on Reduced Graphene Oxide under Facile Thermal Treatment: Microbiological and Cell Assay

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
N. L. V. Carreño ◽  
A. M. Barbosa ◽  
V. C. Duarte ◽  
C. F. Correa ◽  
C. Ferrúa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Graphene Oxide ◽  
Cell Viability ◽  
Reduced Graphene Oxide ◽  
Nanocomposite Films ◽  
Control Group ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Reduced Graphene

Silver-functionalized reduced graphene oxide (Ag-rGO) nanosheets were prepared by single chemical and thermal processes, with very low concentration of silver. The resulting carbon framework consists of reduced graphene oxide (rGO) sheets or 3D networks, decorated with anchored silver nanoparticles. The Ag-rGO nanosheets were dispersed into a polymer matrix and the composites evaluated for use as biological scaffolds. The rGO material in poly(dimethylsiloxane) (PDMS) has been tested for antimicrobial activity against Gram-positiveStaphylococcus aureus(S. Aureus) bacteria, after exposure times of 24 and 120 hours, as well as in the determination of cell viability on cultures of fibroblast cells (NIH/3T3). Using 1 mL of Ag-rGO in PDMS the antibacterial effectiveness againstStaphylococcus aureuswas limited, showing an increased amount of Colony Forming Units (CFU), after 24 hours of contact. In the cell viability assay, after 48 hours of contact, the group of 1 mL of Ag-rGO with PDMS was the only group that increased cell viability when compared to the control group. In this context, it is believed these behaviors are due to the increase in cell adhesion capacity promoted by the rGO. Thus, the Ag-rGO/PDMS hybrid nanocomposite films can be used as scaffolds for tissue engineering, as they limit antimicrobial activity.

scholarly journals Electroactive 3D Printed Scaffolds Based on Percolated Composites of Polycaprolactone with Thermally Reduced Graphene Oxide for Antibacterial and Tissue Engineering Applications

Nanomaterials ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 428 ◽  
Author(s):  
Carolina Angulo-Pineda ◽  
Kasama Srirussamee ◽  
Patricia Palma ◽  
Victor M. Fuenzalida ◽  
Sarah H. Cartmell ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Cell Viability ◽  
Reduced Graphene Oxide ◽  
Human Cell ◽  
Human Mesenchymal Stem Cells ◽  
Fold Increase ◽  
Cellular Mechanisms ◽  
Engineering Applications ◽  
Reduced Graphene

Applying electrical stimulation (ES) could affect different cellular mechanisms, thereby producing a bactericidal effect and an increase in human cell viability. Despite its relevance, this bioelectric effect has been barely reported in percolated conductive biopolymers. In this context, electroactive polycaprolactone (PCL) scaffolds with conductive Thermally Reduced Graphene Oxide (TrGO) nanoparticles were obtained by a 3D printing method. Under direct current (DC) along the percolated scaffolds, a strong antibacterial effect was observed, which completely eradicated S. aureus on the surface of scaffolds. Notably, the same ES regime also produced a four-fold increase in the viability of human mesenchymal stem cells attached to the 3D conductive PCL/TrGO scaffold compared with the pure PCL scaffold. These results have widened the design of novel electroactive composite polymers that could both eliminate the bacteria adhered to the scaffold and increase human cell viability, which have great potential in tissue engineering applications.

Reduced Graphene Oxide-Based Assay for Real-Time Monitoring of Cancer Cell Viability

NANO ◽  
2015 ◽  
Vol 10 (07) ◽  
pp. 1550094
Author(s):  
Jing Wu ◽  
Yang Tang ◽  
Zengliang Shi ◽  
Zhengshan Tian ◽  
Junhua Wang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Cell Viability ◽  
Cancer Cells ◽  
Real Time ◽  
Reduced Graphene Oxide ◽  
Cancer Cell ◽  
Electronic Device ◽  
Physiological State ◽  
Important Indicator ◽  
Reduced Graphene

Viability of cancer cell is an important indicator of physiological state and function of cells, which can be effected by the change of pH in the medium solution, due to the increase of carbon oxide and lactic acid caused by respiration. Although many methods have been developed to detect the viability of cells, mostly based on cytochemical staining and polymerase chain reaction (PCR) technology are time consuming. In this paper, an electronic device was made by thermal reduced graphene oxide (RGO) for detection of cancer cell viability in real-time. This electronic device could be used to monitor the metabolic activity and viability of cancer cells based on the change in pH value. As the pH decreases, colon cancer cells loose viability and the current decreases. This RGO device is simple, sensitive and label-free and could serve as a platform for detection of cells and drug testing.

Effect of the reduced graphene oxide (rGO) compaction degree and concentration on rGO–polymer composite printability and cell interactions

Nanoscale ◽  
2021 ◽  
Author(s):  
María Cámara-Torres ◽  
Ravi Sinha ◽  
Siamak Eqtesadi ◽  
Rune Wendelbo ◽  
Marco Scatto ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Physicochemical Properties ◽  
Cell Viability ◽  
Reduced Graphene Oxide ◽  
Polymer Composite ◽  
Antibacterial Properties ◽  
Cell Interactions ◽  
Support Cell ◽  
Reduced Graphene ◽  
Compaction Degree

rGO densification prior to polymer compounding affects the composite printability and physicochemical properties, which also depend on rGO concentration. The resulting scaffolds support cell viability and osteogenesis, and possess antibacterial properties.

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