scholarly journals Electroactive 3D Printed Scaffolds Based on Percolated Composites of Polycaprolactone with Thermally Reduced Graphene Oxide for Antibacterial and Tissue Engineering Applications

Nanomaterials ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 428 ◽  
Author(s):  
Carolina Angulo-Pineda ◽  
Kasama Srirussamee ◽  
Patricia Palma ◽  
Victor M. Fuenzalida ◽  
Sarah H. Cartmell ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Cell Viability ◽  
Reduced Graphene Oxide ◽  
Human Cell ◽  
Human Mesenchymal Stem Cells ◽  
Fold Increase ◽  
Cellular Mechanisms ◽  
Engineering Applications ◽  
Reduced Graphene

Applying electrical stimulation (ES) could affect different cellular mechanisms, thereby producing a bactericidal effect and an increase in human cell viability. Despite its relevance, this bioelectric effect has been barely reported in percolated conductive biopolymers. In this context, electroactive polycaprolactone (PCL) scaffolds with conductive Thermally Reduced Graphene Oxide (TrGO) nanoparticles were obtained by a 3D printing method. Under direct current (DC) along the percolated scaffolds, a strong antibacterial effect was observed, which completely eradicated S. aureus on the surface of scaffolds. Notably, the same ES regime also produced a four-fold increase in the viability of human mesenchymal stem cells attached to the 3D conductive PCL/TrGO scaffold compared with the pure PCL scaffold. These results have widened the design of novel electroactive composite polymers that could both eliminate the bacteria adhered to the scaffold and increase human cell viability, which have great potential in tissue engineering applications.

Stimulated Osteogenic Differentiation of Human Mesenchymal Stem Cells by Reduced Graphene Oxide

2015 ◽  
Vol 15 (10) ◽  
pp. 7966-7970 ◽  
Author(s):  
Linhua Jin ◽  
Jong Ho Lee ◽  
Oh Seong Jin ◽  
Yong Cheol Shin ◽  
Min Jeong Kim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Osteogenic Differentiation ◽  
Reduced Graphene Oxide ◽  
Human Mesenchymal Stem Cells ◽  
Water Soluble ◽  
Alizarin Red ◽  
Reduced Graphene

Osteoprogenitor cells play a significant role in the growth or repair of bones, and have great potential as cell sources for regenerative medicine and bone tissue engineering, but control of their specific differentiation into bone cells remains a challenge. Graphene-based nanomaterials are attractive candidates for biomedical applications as substrates for stem cell (SC) differentiation, scaffolds in tissue engineering, and components of implant devices owing to their biocompatible, transferable and implantable properties. This study examined the enhanced osteogenic differentiation of human mesenchymal stem cells (hMSCs) by reduced graphene oxide (rGO) nanoparticles (NPs), and rGO NPs was prepared by reducing graphene oxide (GO) with a hydrazine treatment followed by annealing in argon and hydrogen. The cytotoxicity profile of each particle was examined using a water-soluble tetrazolium-8 (WST-8) assay. At different time-points, a WST-8 assay, alkaline phosphatase (ALP) activity assay and alizarin red S (ARS) staining were used to determine the effects of rGO NPs on proliferation, differentiation and mineralization, respectively. The results suggest that graphene-based materials have potential as a platform for stem cells culture and biomedicalapplications.

Preparation of PVA nanocomposites using salep-reduced graphene oxide with enhanced mechanical and biological properties

RSC Advances ◽  
2015 ◽  
Vol 5 (112) ◽  
pp. 92428-92437 ◽  
Author(s):  
Ali Pourjavadi ◽  
Behzad Pourbadiei ◽  
Mohadeseh Doroudian ◽  
Shahram Azari
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Biological Properties ◽  
Engineering Applications ◽  
Reduced Graphene ◽  
Film Nanocomposites

Hydrogel and film nanocomposites based on PVA with desired mechanical properties are prepared using salep functionalized rGO and proposed as new biomaterials for tissue engineering applications.

scholarly journals Reduced graphene oxide-loaded nanocomposite scaffolds for enhancing angiogenesis in tissue engineering applications

2018 ◽  
Vol 5 (5) ◽  
pp. 172017 ◽  
Author(s):  
S. Chakraborty ◽  
T. Ponrasu ◽  
S. Chandel ◽  
M. Dixit ◽  
V. Muthuvijayan
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Chorioallantoic Membrane ◽  
Engineering Applications ◽  
Signalling Molecules ◽  
Cytotoxicity Studies ◽  
Reduced Graphene

Tissue engineering combines cells, scaffolds and signalling molecules to synthesize tissues in vitro . However, the lack of a functioning vascular network severely limits the effective size of a tissue-engineered construct. In this work, we have assessed the potential of reduced graphene oxide (rGO), a non-protein pro-angiogenic moiety, for enhancing angiogenesis in tissue engineering applications. Polyvinyl alcohol/carboxymethyl cellulose (PVA/CMC) scaffolds loaded with different concentrations of rGO nanoparticles were synthesized via lyophilization. Characterization of these scaffolds showed that the rGO-loaded scaffolds retained the thermal and physical properties (swelling, porosity and in vitro biodegradation) of pure PVA/CMC scaffolds. In vitro cytotoxicity studies, using three different cell lines, confirmed that the scaffolds are biocompatible. The scaffolds containing 0.005 and 0.0075% rGO enhanced the proliferation of endothelial cells (EA.hy926) in vitro . In vivo studies using the chick chorioallantoic membrane model showed that the presence of rGO in the PVA/CMC scaffolds significantly enhanced angiogenesis and arteriogenesis.

scholarly journals The influence of reduced graphene oxide on stem cells: a perspective in peripheral nerve regeneration

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Xiangyun Yao ◽  
Zhiwen Yan ◽  
Xu Wang ◽  
Huiquan Jiang ◽  
Yun Qian ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Graphene Oxide ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Reduced Graphene Oxide ◽  
Peripheral Nerve Regeneration ◽  
Embryonic Stem ◽  
Composite Scaffolds ◽  
Reduced Graphene

Abstract Graphene and its derivatives are fascinating materials for their extraordinary electrochemical and mechanical properties. In recent decades, many researchers explored their applications in tissue engineering and regenerative medicine. Reduced graphene oxide (rGO) possesses remarkable structural and functional resemblance to graphene, although some residual oxygen-containing groups and defects exist in the structure. Such structure holds great potential since the remnant-oxygenated groups can further be functionalized or modified. Moreover, oxygen-containing groups can improve the dispersion of rGO in organic or aqueous media. Therefore, it is preferable to utilize rGO in the production of composite materials. The rGO composite scaffolds provide favorable extracellular microenvironment and affect the cellular behavior of cultured cells in the peripheral nerve regeneration. On the one hand, rGO impacts on Schwann cells and neurons which are major components of peripheral nerves. On the other hand, rGO-incorporated composite scaffolds promote the neurogenic differentiation of several stem cells, including embryonic stem cells, mesenchymal stem cells, adipose-derived stem cells and neural stem cells. This review will briefly introduce the production and major properties of rGO, and its potential in modulating the cellular behaviors of specific stem cells. Finally, we present its emerging roles in the production of composite scaffolds for nerve tissue engineering.

Reduced Graphene Oxide Paper: Fabrication by a Green Thermal Reduction Method and Preliminary Study of its In Vitro Cytotoxicity

2017 ◽  
Vol 45 ◽  
pp. 199-207 ◽  
Author(s):  
Xin Wang ◽  
Peng Li ◽  
Claudia Luedecke ◽  
Qiang Zhang ◽  
Zan Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Mammalian Cells ◽  
Reduction Method ◽  
Heating Temperature ◽  
Synthesis Method ◽  
Thermal Reduction ◽  
Graphene Films ◽  
Reduced Graphene

Graphene films have been intensively explored because of their unique mechanical and physicochemical properties for potential applications in field of tissue engineering and implants. However, for biomedical applications, it is necessary to fully understand the toxicity and biocompatibility of the prepared graphene films since different synthesis method might lead to different biological properties. Here we report a step-by-step thermal reduction method of preparing reduced graphene oxide (rGO) film directly on various substrates at low heating temperature (below about 200 °C) without requiring any chemical reduction agent like hydrazine or other reductants (therefore we call it green method). Slowly heating GO hydrosol that was coated on the surface of a glass cell-culture dish or inside of a polypropylene tube from room temperature to 60, 100, and 160 °C for 12 h, respectively, a shiny and flat surface without crumpled structure or tiny pores was formed. We peeled it off from the substrate to explore its cytotoxicity. The results exhibited that the rGO film was biocompatible with Cal-72 cell but against Escherichia coli bacteria. Our work confirmed that rGO film produced by the green reduction method is cytocompatible with mammalian cells, which makes this rGO film a promising material for tissue engineering scaffold or as a surface-modification coating of an implant.

Reduced graphene oxide-coated hydroxyapatite composites stimulate spontaneous osteogenic differentiation of human mesenchymal stem cells

Nanoscale ◽  
2015 ◽  
Vol 7 (27) ◽  
pp. 11642-11651 ◽  
Author(s):  
Jong Ho Lee ◽  
Yong Cheol Shin ◽  
Oh Seong Jin ◽  
Seok Hee Kang ◽  
Yu-Shik Hwang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Osteogenic Differentiation ◽  
Reduced Graphene Oxide ◽  
Human Mesenchymal Stem Cells ◽  
Reduced Graphene

Reduced graphene oxide-coated hydroxyapatite (rGO-coated HAp) composites stimulated the spontaneous osteogenesis in human mesenchymal stem cells in the absence of osteoinductive agents.

scholarly journals Reduced Graphene Oxide-GelMA Hybrid Hydrogels as Scaffolds for Cardiac Tissue Engineering

Small ◽  
2016 ◽  
Vol 12 (27) ◽  
pp. 3677-3689 ◽  
Author(s):  
Su Ryon Shin ◽  
Claudio Zihlmann ◽  
Mohsen Akbari ◽  
Pribpandao Assawes ◽  
Louis Cheung ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Hybrid Hydrogels ◽  
Reduced Graphene

scholarly journals Metal-Carbon Interactions on Reduced Graphene Oxide under Facile Thermal Treatment: Microbiological and Cell Assay

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
N. L. V. Carreño ◽  
A. M. Barbosa ◽  
V. C. Duarte ◽  
C. F. Correa ◽  
C. Ferrúa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Graphene Oxide ◽  
Cell Viability ◽  
Reduced Graphene Oxide ◽  
Nanocomposite Films ◽  
Control Group ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Reduced Graphene

Silver-functionalized reduced graphene oxide (Ag-rGO) nanosheets were prepared by single chemical and thermal processes, with very low concentration of silver. The resulting carbon framework consists of reduced graphene oxide (rGO) sheets or 3D networks, decorated with anchored silver nanoparticles. The Ag-rGO nanosheets were dispersed into a polymer matrix and the composites evaluated for use as biological scaffolds. The rGO material in poly(dimethylsiloxane) (PDMS) has been tested for antimicrobial activity against Gram-positiveStaphylococcus aureus(S. Aureus) bacteria, after exposure times of 24 and 120 hours, as well as in the determination of cell viability on cultures of fibroblast cells (NIH/3T3). Using 1 mL of Ag-rGO in PDMS the antibacterial effectiveness againstStaphylococcus aureuswas limited, showing an increased amount of Colony Forming Units (CFU), after 24 hours of contact. In the cell viability assay, after 48 hours of contact, the group of 1 mL of Ag-rGO with PDMS was the only group that increased cell viability when compared to the control group. In this context, it is believed these behaviors are due to the increase in cell adhesion capacity promoted by the rGO. Thus, the Ag-rGO/PDMS hybrid nanocomposite films can be used as scaffolds for tissue engineering, as they limit antimicrobial activity.

Sign in / Sign up

Export Citation Format

Share Document