scholarly journals Cross-linked polymersomes as nanoreactors for controlled and stabilized single and cascade enzymatic reactions

Nanoscale ◽  
2014 ◽  
Vol 6 (18) ◽  
pp. 10752-10761 ◽  
Author(s):  
David Gräfe ◽  
Jens Gaitzsch ◽  
Dietmar Appelhans ◽  
Brigitte Voit

Cross-linked polymersomes are used as nanoreactors for an enzyme cascade reaction between different polymersomes. For the first time the polymersomes show a great stabilization effect on the enclosed enzymes in comparison to free ones. Thus, the pH value is used to switch the reaction on and off several times.

2021 ◽  
pp. 101066
Author(s):  
Luhua Zheng ◽  
Bo Jiang ◽  
Jingjing Chen ◽  
Tao Zhang ◽  
Xiaohong Gu ◽  
...  

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1165 ◽  
Author(s):  
Andrew Hamilton ◽  
Chris Davison ◽  
Christos Tachtatzis ◽  
Ivan Andonovic ◽  
Craig Michie ◽  
...  

The reticuloruminal function is central to the digestive efficiency in ruminants. For cattle, collar- and ear tag-based accelerometer monitors have been developed to assess the time spent ruminating on an individual animal. Cattle that are ill feed less and so ruminate less, thus, the estimation of the time spent ruminating provides insights into the health of individual animals. pH boluses directly provide information on the reticuloruminal function within the rumen and extended (three hours or more) periods during which the ruminal pH value remains below 5.6 is an indicator that dysfunction and poor welfare are likely. Accelerometers, incorporated into the pH boluses, have been used to indicate changes in behaviour patterns (high/low activity), utilised to detect the onset of oestrus. The paper demonstrates for the first time that by processing the reticuloruminal motion, it is possible to recover rumination periods. Reticuloruminal motion energy and the time between reticuloruminal contractions are used as inputs to a Support Vector Machine (SVM) to identify rumination periods with an overall accuracy of 86.1%, corroborated by neck mounted rumination collars.


2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Yu Zheng ◽  
Xudong Luo ◽  
Jinlong Yang ◽  
Wenlong Huo ◽  
Chi Kang

A novel approach is used for fabricating steel slag foam ceramics based on the particle-stabilized foaming method. In this work, steel slag was used as the raw material and propyl gallate (PG) was used as the surface modifier. For the first time, steel slag ceramic foams were successfully fabricated based on particle-stabilized foams. The results show that the stability of the ceramic foams was closely related to the pH value and PG concentration. The porosity and compressive strength could be controlled by changing the solid loading of steel slag and sintering temperature. The porosity of steel slag foam ceramics ranged from 85.6% to 62.53%, and the compressive strength was from 1.74 MPa to 10.42 MPa. The thermal conductivity of steel slag foam ceramics was only 0.067 W (m·K)−1, which shows that it could be used as a thermal insulation material.


2018 ◽  
Author(s):  
Wanyun Xu ◽  
Ye Kuang ◽  
Chunsheng Zhao ◽  
Jiangchuan Tao ◽  
Gang Zhao ◽  
...  

Abstract. The study of atmospheric nitrous acid (HONO), which is the primary source of OH radicals, is crucial to atmospheric photochemistry and heterogeneous chemical processes. The heterogeneous NO2 chemistry under haze conditions was pointed out to be one of the missing sources of HONO on the North China Plain, producing sulfate and nitrate in the process. However, controversy exists between various proposed mechanisms, mainly debating on whether SO2 directly takes part in the HONO production process and what roles NH3 and the pH value play in it. In this paper, never before seen explosive HONO production (maximum rate: 16 ppb/hour) was reported and evidence was found for the first time in field measurements during fog episodes (usually with pH > 5) and haze episodes under high relative humidity (usually with pH 


2011 ◽  
Vol 214 ◽  
pp. 240-244
Author(s):  
Yi Nong Yan ◽  
Xiang Ru Zhang ◽  
Dan Li ◽  
Jian Jian Xu

Pretreatment solution which was obtained by many experiments who had a good printing effect was used to do the digital printing experiment on the cashmere fabric. Printing performance of the cashmere fabric in different pH value was compared to improve the printing quality of the cashmere fabric. Digital printing experiments in the alkaline environment were carried out for the first time and the best pH value was found out which could ensure dye uptake, color fastness, anti-seepage property and minimal damage to cashmere fabric. The paper provided basic reference to the actual production.


2021 ◽  
Vol 213 (10) ◽  
pp. 75-80
Author(s):  
E. Razhina

Abstract. The aim of this work is to investigate the relationship between indicators of cicatricial digestion and linearity of cows. Methods. The research was carried out in the breeding enterprises of the Sverdlovsk region on the livestock of Ural-type cows. Cicatricial fluid was collected with an oropharyngeal rubber probe, and a wooden yaw was also used. Cicatricial content was assessed at the Chelyabinsk Interregional Veterinary Laboratory. The pH value was determined by the electrometric method, VFA – in the Markgam apparatus, ammonia – by the microdiffusion method. Ciliates – in Goryaev’s chamber and under a microscope, bacteria – under a microscope with the addition of sodium chloride solution. Statistical data processing was carried out in the Microsoft Office Excel 2010 program. Results. In terms of the number of ciliates and bacteria in the rumen fluid, the Montwick Chieftain cows, characterized by high productive qualities, had an advantage. In terms of pH and VFA, the Vis Back Ideal line was the best. There were no significant differences between the groups in terms of the amount of ammonia in the rumen. The best animals from the point of view of influence on cicatricial metabolism were the animals of the Vis Back Ideal and Montwick Chieftain lineage. Scientific novelty. Studies have shown that linearity is associated with cicatricial digestion in cows. The relationship of bull lines with indicators of cicatricial metabolism of cows has been determined for the first time. The food in the rumen is digested due to the action of bacteria, ciliates, and fungi. An environment has been created in the rumen for the active development of microflora. Bacteria are able to synthesize amino acids and vitamins. The proventriculus can contain up to 50 species of ciliates.


RSC Advances ◽  
2020 ◽  
Vol 10 (32) ◽  
pp. 18655-18676 ◽  
Author(s):  
Nicolas Ghéczy ◽  
Kai Sasaki ◽  
Makoto Yoshimoto ◽  
Sajad Pour-Esmaeil ◽  
Martin Kröger ◽  
...  

A cascade reaction catalysed by bovine carbonic anhydrase (BCA) and horseradish peroxidase (HRP) proceeds over two possible pathways, which explains differences in product formation for differently immobilised enzymes in flow-through reactions.


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